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EC number: 238-056-5 | CAS number: 14205-39-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January - March 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Test item code: LZ937.00
Expiration date: 21 May 2000
Delivery date: 11 October 1999
Test material was stored at ambient temperature in the dark.
Physical state: White crystalline solid
Stoarge conditions: Stored at ambient temperature in the dark - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- RANGE FINDING TEST:
Test solutions were prepared by adding a weighed amount of LZ937 (10.00880, 1.0025, 0.0997, 0.01022 g) directly to the appropriate test tank (1000, 100, 10 and 1 ppm respectively). Each test tank contained 10 litres of test media, and the diluent medium was reconstituted freshwater. The tank containing 1000 ppm solution was then ultrasonicated for 5 min to aid solubility.
DEFINITIVE TEST:
The test solutions were prepared by removing a small volume of water from the appropriate test tank. A pre-weighed amount of LZ937 was added and then ultrasonicated for ca 1-2 min to aid dissolution, before being returned to the appropriate tank. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Rainbow Trout (Oncorhynchus mykiss) were used for this study and were received from Dennington Trout Farm, on the 19th January 2000. All fish were of the same year class and source. All fish were in good health and free from any apparent malformation. All fish were acclimatised to laboratory conditions for at least 12 days prior to commencement of the study. During holding the fish were fed on a suitable standard fish diet. The fish were not fed for the period 24 h before the commencement of the tests or throughout the duration of the tests.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- average 74 mg CaCO3
- Test temperature:
- 14.3°C - 15.1°C
- pH:
- 7.54 - 8.12
- Dissolved oxygen:
- > 93.2%
- Salinity:
- not applicable
- Conductivity:
- 250 - 312 µS
- Nominal and measured concentrations:
- Range finding: 1000, 100, 10 and 1 p.p.m. with an untreated control (0 p.p.m.).
Definitive test: 100, 50, 25, 12.5, and 6.25 p.p.m. nominal concentrations - Details on test conditions:
- Glass tanks (ca 25 litre capacity), covered with perspex lids to minimise dust contamination and evaporation loss, were used as the test vessels. Holding, and all test tanks were filled using reconstituted freshwater suitable for holding fish and produced in the Ecotoxicology facility at lnveresk Research. This was a deviation from the protocol which stated dechlorinated tap water. This deviation is not considered to have affected the validity of the
test. Test tanks were maintained within a temperature-controlled laboratory at approximately 15°C. A light cycle of 16 h light and 8 h dark was in operation throughout the tests. Fluorescent tubes provided artificial daylight illumination. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 35.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- It was observed that 100% mortality occurred in the 1000 and 100 p.p.m. test vessels after 24 h. No further mortality was observed at any other concentration. Effects were observed within 1 h of exposure with fish in the 100 p.p.m. tank beginning to exhibit a loss of equilibrium. After 2 h, all fish in the 100 p.p.m. tank had died and one fish in the 50 p.p.m. tank was starting to exhibit a loss of equilibrium whilst swimming. By 24 h all the fish in the 50 p.p.m. tank had died and the fish in the 25 p.p.m. tank were appearing discoloured and lethargic.Two fish escaped from the tank containing 12.5 p.p.m. of LZ937 and one fish from the tank containing 6.25 p.p.m. LZ937. It is unlikely that this was an effect of the test and these fish were excluded from any calculation of results.
- Reported statistics and error estimates:
- The LC50 value was estimated based on the geometric mean of the consecutive nominal test concentrations, at which 100 % mortality and no mortality were observed (50 and 25 p.p.m. respectively). This method was applied as conventional probit analysis is not suitable when intermediate values of effect are absent. Similarly the 95 % confidence intervals are conservatively estimated to be the consecutive concentrations at which 0 and 100% effect were observed. The No Observed Effect Concentration (NOEC) based on observation was 25 p.p.m., nominal, under the conditions of the test.
- Sublethal observations / clinical signs:
All the tanks were clear and colourless, throughout the duration of the study. No precipitation of test material was observed. This indicates full dispersion or dissolution of the test material in the test medium. All results are based on the nominal concentrations in the absence of analytical results.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96h LC50 is 35.4 mg/L based on nominal concentrations of LZ937.
- Executive summary:
The study was conducted to determine the acute toxicity (LC50) of LZ937 to the fresh water fish Rainbow Trout (Oncorhynchus mykiss). The study was conducted under semi-static conditions for 96 h in accordance
with OECD Guideline 203 for Testing of Chemicals and EC Guideline C 1. Following a preliminary range finding study a definitive test was conducted at 100, 50, 25, 12.5, and 6.25 p.p.m. nominal concentrations of LZ937.
Exposure solutions were replaced after every 24h period. Ten fish were exposed at each concentration in a single replicate vessel. The 96h LC50 is 35.4 mg/L based on nominal concentrations of LZ937.
Development of an analytical method was not possible.
Reference
Description of key information
The study was conducted to determine the acute toxicity (LC50) of LZ937 to the fresh water fish Rainbow Trout (Oncorhynchus mykiss). The study was conducted under semi-static conditions for 96 h in accordance
with OECD Guideline 203 for Testing of Chemicals and EC Guideline C 1. Following a preliminary range finding study a definitive test was conducted at 100, 50, 25, 12.5, and 6.25 ppm nominal concentrations of LZ937.
Exposure solutions were replaced after every 24h period. Ten fish were exposed at each concentration in a single replicate vessel.
The 96h LC50 is 35.4 mg/L based on nominal concentrations of LZ937.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 35.4 mg/L
Additional information
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