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EC number: 213-149-3 | CAS number: 927-20-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 - 08 - 2017 - 27 - 10 - 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Magnesium glycerophosphate
- EC Number:
- 213-149-3
- EC Name:
- Magnesium glycerophosphate
- Cas Number:
- 927-20-8
- Molecular formula:
- C3H9O6P.Mg
- IUPAC Name:
- magnesium glycerophosphate
- Test material form:
- solid: particulate/powder
- Details on test material:
- Batch: INVG003917
Constituent 1
- Specific details on test material used for the study:
- Name: Magnesium Glycerophosphate
Synonym: Magna-C
Batch No.: INVG003917
Composition: 96.5 %(w/w) (calculated)
Magnesium 2,3-dihydroxypropyl phosphate:
96.5% (w/w)
Aggregate State at Room Temperature: Solid
Colour: White
Molecular Formula / Molecular Weight C3H7O6P Mg/ 194.36 g/mol
Theoretical Oxygen Demand: ThODNH4: 0.494 mg oxygen per mg test item
Expiration Date: June 10, 2021
Storage Conditions at Test Facility: At 20 5 °C, in the dark
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Aerobic activated sludge, microorganisms from a domestic waste water treatment plant was supplied by the sewage treatment plant Bensheim, Germany.
The aerobic activated sludge used for this study was deposited for 30 min, washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in test water and centrifuged again. This procedure was done three times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material per litre were mixed with test water and aerated overnight. This suspension was used for the experiment. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- ca. 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The test item Magnesium Glycerophosphate was investigated for its ready biodegradability in a manometric respirometry test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
Test Units:
Manometric Test System with test flasks containing a volume of approximately 500 mL.
BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany
Principle:
The test flasks prepared according paragraph 6.6 were incubated at 22°C * 1°C. The pressure decrease in the reaction vessels was measured over complete experimental phase of 28 days using the BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany. The test flasks were closed gas-tight by a measuring head. Potassium hydroxide solution (45%) was used for trapping the produced carbon dioxide. The amount of O2 consumed by the activated sludge was calculated from the decrease of pressure in the reaction vessel.
Preparation of test flasks:
The amounts of test item and reference item were directly weighed into the test flasks. No emulsifiers and solvents were used.
Incubation:
The cllosed test flasks were incubated in a climatised chamber under continuous stirring. The consumption of oxygen was determined by measuring the change of pressure in tthe flasks. Evolved carbon dioxicd was absorbed in an aqueous solution (45%) of potassium hydroxide.
Test duration:
28days
Measurement of oxygen:
The change of pressure in the test flasks was measured by means of a manometric method (BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany) each day.
Temperature:
The temperature was recorded by means of the automated sooftware AMR Wincontrol ©.
pH-Value:
pH-values were measured in procedure control, a separately prepared test flask with test item (to prevent
loss of test item in the test flasks) and a separately prepared test flask without test item (control) at test start and in all flasks at the end of the test, except in the abiotic and toxicity control, using a pH-electrode
WTW pH 340i.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- The degradation rate of Magnesium Glycerophosphate did not reach 60% within the 10-day window, but after 28 days.
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 85
- Sampling time:
- 28 d
- Details on results:
- The 10-day windows began on day 1 after application, the mean value was calculated to be 15% biodegradation. Therefore, the end of the 10-day window was day 11. After correction for the mean biochemical oxygen demand of the inoculum controls the mean biodegradation percentage based on ThODNH4 at the end of the 10-day window was 50%; the criterion of the 10 day window was not passed. The mean biodegradation percentage at the end of the 28-day exposure period was 85%.
The degradation rate of Magnesium Glycerophosphate did not reach 60% within the 10-day window, but after 28 days.
Therefore, Magnesium Glycerophosphate is considered to be readily biodegradable after 28 days, but failing the 10-day window criterion.
BOD5 / COD results
- Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 80% after 14 days and to 83% after 28 days of incubation.
The percentage biodegradation of the reference item confirms the suitability of the used aerobic activated sludge inoculum.
Any other information on results incl. tables
Table2. Cumulative Biochemical Oxygen Demand (mg O2/L) in Test Flasks during the Test Period of 28 Days
Time |
Flask No. |
|||||||
(days) |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
|
1 |
5 |
15 |
0 |
5 |
50 |
0 |
80 |
|
2 |
10 |
25 |
5 |
5 |
80 |
0 |
115 |
|
3 |
15 |
30 |
5 |
10 |
110 |
0 |
125 |
|
4 |
20 |
35 |
10 |
10 |
120 |
0 |
130 |
|
5 |
25 |
35 |
10 |
10 |
125 |
0 |
135 |
|
6 |
30 |
35 |
10 |
10 |
130 |
0 |
150 |
|
7 |
30 |
35 |
15 |
15 |
135 |
0 |
155 |
|
8 |
35 |
35 |
15 |
15 |
140 |
0 |
160 |
|
9 |
40 |
40 |
15 |
15 |
145 |
0 |
170 |
|
10 |
40 |
40 |
15 |
20 |
150 |
0 |
175 |
|
11 |
45 |
40 |
15 |
20 |
150 |
0 |
180 |
|
12 |
45 |
40 |
20 |
20 |
150 |
0 |
180 |
|
13 |
50 |
40 |
20 |
20 |
155 |
0 |
190 |
|
14 |
50 |
40 |
20 |
20 |
155 |
0 |
190 |
|
15 |
50 |
40 |
20 |
20 |
155 |
0 |
190 |
|
16 |
55 |
45 |
20 |
20 |
155 |
0 |
195 |
|
17 |
55 |
45 |
20 |
20 |
160 |
0 |
195 |
|
18 |
55 |
45 |
20 |
25 |
160 |
0 |
195 |
|
19 |
55 |
45 |
20 |
25 |
160 |
0 |
195 |
|
20 |
55 |
45 |
20 |
25 |
160 |
0 |
200 |
|
21 |
60 |
45 |
20 |
25 |
160 |
0 |
205 |
|
22 |
60 |
45 |
20 |
25 |
160 |
0 |
205 |
|
23 |
60 |
55 |
20 |
25 |
165 |
0 |
210 |
|
24 |
60 |
55 |
20 |
25 |
165 |
0 |
215 |
|
25 |
60 |
55 |
20 |
25 |
165 |
0 |
215 |
|
26 |
65 |
55 |
20 |
25 |
165 |
0 |
215 |
|
27 |
65 |
55 |
20 |
30 |
165 |
0 |
215 |
|
28 |
65 |
70 |
20 |
30 |
165 |
0 |
215 |
|
Flasks 1 and 2: Magnesium Glycerophosphate
Flasks 3 and 4: inoculum control
Flask 5:reference(procedure control)
Flask 6: abiotic control
Flask 7: toxicity control
Table3. Percentage Biodegradation of Test Item, of Sodium Benzoate and of the Toxicity Control based on ThODNH4
Time |
Percentage Biodegradation1 |
|||
(Days) |
Magnesium Glycerophosphate1 |
Sodium Benzoate2 |
Toxicity |
|
|
Flask 1 [%] |
Flask 2 [%] |
Flask 5 [%] |
Flask 7 [%] |
1 |
5 |
25 |
28 |
35 |
2 |
10 |
40 |
44 |
50 |
3 |
15 |
45 |
61 |
53 |
4 |
20 |
50 |
65 |
54 |
5 |
30 |
50 |
68 |
57 |
6 |
39 |
50 |
71 |
63 |
7 |
30 |
40 |
71 |
63 |
8 |
39 |
40 |
74 |
66 |
9 |
49 |
50 |
77 |
70 |
10 |
44 |
45 |
79 |
71 |
11 |
54 |
45 |
79 |
73 |
12 |
49 |
40 |
77 |
72 |
13 |
59 |
40 |
80 |
77 |
14 |
59 |
40 |
80 |
77 |
15 |
59 |
40 |
80 |
77 |
16 |
69 |
50 |
80 |
79 |
17 |
69 |
50 |
83 |
79 |
18 |
64 |
45 |
82 |
78 |
19 |
64 |
45 |
82 |
78 |
20 |
64 |
45 |
82 |
80 |
21 |
74 |
45 |
82 |
83 |
22 |
74 |
45 |
82 |
83 |
23 |
74 |
65 |
84 |
85 |
24 |
74 |
65 |
84 |
87 |
25 |
74 |
65 |
84 |
87 |
26 |
84 |
65 |
84 |
87 |
27 |
79 |
60 |
83 |
86 |
28 |
79 |
90 |
83 |
86 |
1ThODNH4of
Magnesium Glycerophosphate: 0.494 mg O2/mg test item
2ThODNH4of
sodium benzoate: 1.666 mg O2/mg reference item
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Conclusions:
- Biodegradation Test Item:
The degradation rate of Magnesium Glycerophosphate did not reach 60% within the 10-day window, but after 28 days.
Therefore, Magnesium Glycerophosphate is considered to be readily biodegradable after 28 days, but failing the 10-day window criterion.
Biodegradation Reference Item:
The reference item sodium benzoate was sufficiently degraded to 80% after 14 days and to 83% after 28 days of incubation.
The percentage biodegradation of the reference item confirms the suitability of the used aerobic activated sludge inoculum. - Executive summary:
Title:
Magnesium Glycerophosphate: Ready Biodegradability in a Manometric Respirometry Test
Guidelines:
This study was designed to comply with the following methods:
- Commission Regulation 440/2008/EC, Method C.4-D of: Manometric Respirometry Test (EEC Publication No. L 142/496, May 2008)
- OECD Guideline for Testing of Chemicals No. 301 F: "Ready Biodegradability: Manometric Respirometry Test", adopted
Material and Methods:
Test Item:
Magnesium Glycerophosphate; Batch No.:INVG003917
Test Species:
Aerobic activated sludge (microorganisms from a domestic wastewater treatment plant) was supplied by the sewage treatment plant of Bensheim,.
Test Design:
The test item Magnesium Glycerophosphate was investigated for its ready biodegradability in a manometric respirometry test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
Endpoints:
Degradation rate of test item calculated by the oxygen consumption of the aerobic activated sludge microorganisms after 28 days of incubation.
Test Item Loading Rate (initial concentration in medium C0):
102.0 mg/L corresponding to an oxygen demand of about 50.4 mg/L (ThODNH4)
Reference Item:
Sodium Benzoate
Reference Item Loading Rate:
101.2 mg/L corresponding to an oxygen demand of about 168.6 mg/L (ThODNH4)
Test Conditions:
22°C ± 1°C, darkness
Results:
Biodegradation of Magnesium Glycerophosphate:
The criterion for ready biodegradability under the conditions of a manometric respirometry test is the degradation of the test item of at least 60%, reached within a 10-day window; the 10-day window starts when the degradation of the test item reaches at least 10% degradation.
The mean biodegradation of 10% of Magnesium Glycerophosphate was reached at day 1 (ThODNH4). At the end of the 10-day window at day 11, the mean degradation of Magnesium Glycerophosphate was 50% (ThODNH4) and therefore the 10 day window criterion was not passed. The mean biodegradation at test end after 28 days was 85% (ThODNH4).
Therefore, Magnesium Glycerophosphate is considered to be readily biodegradable after 28 days, but failing the 10-day window.
Biodegradation of Sodium Benzoate:
The reference item sodium benzoate was sufficiently degraded to 80% after 14 days and to 83% after 28 days of incubation, thus confirming the suitability of the aerobic activated sludge inoculum used.
Biodegradation of the Toxicity Control:
In the toxicity control containing both, the test item and the reference item sodium benzoate, 77% biodegradation was noted within 14 days and 86% biodegradation after 28 days of incubation. According to the test guidelines, the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days.
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