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EC number: 833-005-9 | CAS number: 1003218-33-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2020-03-17 to 2020-04-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- July, 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Based on the results of the preliminary test it was concluded that the heterotrophic respiration as well as the nitrification are not inhibited. Therefore, the definitive study was carried out with the concentration of 1000 mg/L in 3 replicates (limit test to determine the NOEC).
The test item was weighed out directly in Erlenmeyer flasks and stirred overnight in 200 mL ultrapure water. Triplicates of the control without test item were included at the beginning and at the end of the test.
The reference item was tested with three concentrations with the concentration range 58 - 180 mg/L and a dilution factor of approx. 1.8.
A fresh sample of activated sludge taken from the sewage plant Hildesheim was used for the test procedure. It was washed twice and adjusted with chlorine free tap water to a dry sludge
concentration of 3.0 g/L ± 13 %. The oxygen uptake rates of all samples were determined following an incubation period of 3 h. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Test System: Activated sludge
Origin: Municipal sewage treatment plant of 31137 Hildesheim, Germany
Reason for the selection: Non-adapted activated sludge from the sewage plant at Hildesheim is well suited as it receives predominantly municipal sewage and hardly any industrial chemical waste.
Pretreatment: The sludge was washed twice with chlorine free tap water and adjusted to a dry sludge concentration of 3.0 g/L ± 13 %. The sludge was used within 24 h after sampling.
Date of receipt: 2020-03-12
Dry sludge concentration: 3.39 g/L ( corresponding to 1.70 g/L suspended solids in the test vessels) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21.5°C
- pH:
- pH of activated sludge: 7.39
pH of synthetic waste water: 7.24 - Dissolved oxygen:
- Control samples : 6.11 - 7.39 mg/O2/L
- Nominal and measured concentrations:
- Range Finder: 10, 100 and 1000 mg/L
Main study: 1000 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL Erlenmeyer flasks, DIN 12380
- Type: open
- Aeration:compressed air supply
- No. of vessels per test item concentration (replicates): 5
- No. of vessels of positive control (replicates): 3
- No. of vessels of control (replicates): 6
- Sludge concentration (weight of dry solids per volume): 3.39 g/L
- Nutrients provided for bacteria: no
- Nitrification inhibitor used (delete if not applicable): N-allylthiourea (only one replicate of the dose range finding study)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1000 mg/L
- Results used to determine the conditions for the definitive study: range finding test - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate (CAS No. 7758-99-8)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Results with reference substance (positive control):
- copper-(II)-sulphate pentahydrate
EC50 = 95.3 mg/L, 95% range: (89.8-101.0) mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The test item caused no inhibiting effects on activated sludge of a municipal sewage treatment plant. The EC50 value exceeded the limit concentration of 1000 mg/L.
- Executive summary:
Purpose
The aim of this study was to determine the no observable effect concentration (NOEC) and estimate the effect concentration for an x% effect (ECx) for activated sludge exposed to the test substance, where possible.
Study Design
The study was performed according to OECD Guideline 209, Activated Sludge, Respiration and Inhibition Test (Carbon and Ammonium Oxidation), adopted July, 2010.
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 (2010) was carried out with the test item. The test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out as limit test under static conditions with the test item concentration of 1000 mg/L.
Results
The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicate was -2%.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 95.3 mg/L. The validity criteria of the test guideline were fulfilled.
EC10 > 1000 mg/L
EC50 > 1000 mg/L
NOEC >= 1000 mg/L
Conclusions
The test item caused no inhibiting effects on activated sludge of a municipal sewage treatment plant. The EC50 value exceeded the limit concentration of 1000 mg/L.
Reference
Description of key information
The test item caused no inhibiting effects on activated sludge of a municipal sewage treatment plant. The EC50 and EC10 value exceeded the limit concentration of 1000 mg/L. The NOEC was determined to be 1000 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
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