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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental phase: 08 January 2018 to 14 March 2018. Report issued: 01 August 2018.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- yes
- Remarks:
- The deviations were not considered to have impacted on the conclusions or validity of the study.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test concentrations to be used in the definitive test were determined by a preliminary range-finding test. In the range-finding test, activated sewage sludge micro-organisms were exposed to a series of nominal test concentrations of 10, 100 and 1000 mg/L. The test item was dispersed directly in water.
For the definitive test nominal amounts of test item (50, 160 and 500 mg (five replicates of each)) were each separately dispersed in approximately 200 mL of deionised reverse osmosis water and subjected to ultrasonication for approximately 15 minutes followed by magnetic stirring for 24 hours, at room temperature, in order to maximise the dissolved test item concentration. All test vessels were shielded from the light during mixing. Synthetic sewage (16 mL), activated sewage sludge (250 mL) and water were added to a final volume of 500 mL to give the required concentrations of 100, 320, 1000, mg/L (five replicates of each).
The pH of the test item dispersions was measured after stirring using a handheld pH meter and if necessary, adjusted to between pH 7.0 and 8.0
The control group was maintained under identical conditions but not exposed to the test item. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture:
- Name and location of sewage treatment plant where inoculum was collected: A mixed population of activated sewage sludge micro-organisms was obtained on 08 January 2018 for the range-finding test and on 13 March 2018 for the definitive test from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK which treats predominantly domestic sewage.
- Method of cultivation: On the day of collection the activated sewage sludge (7 litres) was fed synthetic sewage (350 mL).
- Preparation of inoculum for exposure: The activated sewage sludge sample was maintained on continuous aeration in the laboratory at a temperature of approximately 21 ºC overnight prior to use in the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- None
- Test temperature:
- 20 -21 °C
- pH:
- 7.3 - 8.0 at the start of the test and 7.1 - 7.9 at 3 hours (end of the test)
- Dissolved oxygen:
- 5.41 - 8.39 mg O2/L
- Nominal and measured concentrations:
- Definitive test concentrations were 100, 320 and 1000 mg/L of test substance based on nominal concentrations.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 ml conical flasks
- Aeration: continuous aeration
- No. of vessels per concentration (replicates): Five for each test concentration (100, 320 and 1000 mg/L)
- No. of vessels per control (replicates): Six
- No of vesseels referecne substance: one for each concentration.
- Sludge concentration (weight of dry solids per volume): 3.0 g/L prior to use
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The test water used for the test was deionised reverse osmosis water containing less than 1 mg/L Total Organic Carbon (TOC).
OTHER TEST CONDITIONS
- Adjustment of pH: The pH of the test item stock solutions were measured using a handheld meter and if necessary, adjusted to between pH 7.0 to pH 8.0.
- Photoperiod: The test was conducted under normal laboratory lighting - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- The EC50 value (3-hhour contact time) for the reference item, 3,5-Dichlorophenol was 9.0 mg/L, 95% confidence limits 7.3 to 11 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance gave a 3-Hour EC50 value of greater than 1000 mg/L when exposed to activated sludge. The No Observed Effect Concentration (NOEC) after 3 hours exposure was also > 1000 mg/L.
- Executive summary:
Introduction
The study performed to assess the effect of the test substance on the respiration of activated sewage sludge. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2010) No. 209 "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)".
Methods
Activated sewage sludge was exposed to an aqueous solution of the test item at concentrations of 100, 320 and 1000 mg/L (three replicates of the 1000 mg/L test concentration) for a period of 3 hours at a measured temperature of between 20 °C and 21 °C with the addition of a synthetic sewage as a respiratory substrate.
The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5-Dichlorophenol.
Results and Conclusion
The test substance gave a 3-Hour EC50 value of greater than 1000 mg/L when exposed to activated sludge. The No Observed Effect Concentration (NOEC) after 3 hours exposure was also > 1000 mg/L.
The reference item gave a 3-Hour EC50 value of 9.0 mg/L, 95% confidence limits 7.3 to 11 mg/L.
Reference
Range-finder Test
No statistically significant toxic effects were shown at the test concentrations of 10 and 100 mg/L, however statistically significant toxic effects were shown at the test concentration of 1000 mg/L. Based on these results and after discussion with the Sponsor, it was considered necessary to perform a definitive test in order to obtain a NOEC value for the test item. Test concentrations of 100, 320 and 1000 mg/L were selected for the definitive test.
Description of key information
Toxicity of the test substance to activated sludge.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.