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Diss Factsheets
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EC number: 807-935-0 | CAS number: 1244733-77-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Product name: TCPP
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of the test solutions were collected at approximately 0 and 72 hours to measure concentrations of the test substance. Samples at test initiation were collected from the individual batches of test solution prepared for each treatment and control group prior to distribution to the test chambers. At test termination, samples were collected from the pooled replicates of each treatment and control group. All samples were collected in glass scintillation vials and analyzed immediately without storage.
- Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Original algal cultures were obtained from the University of Toronto Culture Collection (UTCC), and had been maintained in culture medium at
Wildlife International, Ltd., Easton, Maryland. Algal cells used in this test were obtained from Wildlife International, Ltd. cultures that had been actively growing in culture medium for at least two weeks prior to test initiation, and had been transferred to fresh medium four days prior to test initiation. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23.2 - 24.6
- pH:
- 7.8 - 7.9 at the beginning of the test
and ranged from 8.0 - 8.9 at test termination. - Nominal and measured concentrations:
- Nominal test concentrations selected were: 6.3, 13, 25, 50, 100 and 200 mg/L
Measured test concentrations were determined from samples of test medium collected from each treatment and control group at the beginning and
end of the test. Mean conc.: 6.5, 13, 25, 46, 88 and 200 mg/L - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 42 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 82 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: (95 % CL) 76 - 89 mg/l
- Details on results:
- Results are expressed relative to nominal concentrations because measured concentrations were within 80-120% of nominal.
- Validity criteria fulfilled:
- yes
- Remarks:
- 1) Mean cell density in the control flasks increased by a factor greater then 16 within three days. 2) The pH of the control did not deviate by more than 1.5 units during the test, and 3) Coefficient of variationof growth rate in control replicates < 35%
- Conclusions:
- A toxicity of TCPP on algae (Pseudokirchnerella subcapitata) of 72 h EC50 of 82 mg/L and an EC10 of 42 mg/L based on growth rate were determined.
- Executive summary:
A study on toxicity to the freshwater algae Pseudokirchnerella subcapitata of TCPP was performed according to OECD Guideline 201 (Alga, Growth Inhibition Test) with analytical monitoring. The test gave an EC50 and an EC10 value for effects on growth of 82 mg/L and 42 mg/l, respectively and a NOEC of 13 mg/l after a test duration of 72 hours, relating to nominal concentrations as the test concentrations were analytically confirmed (Desjardins, 2004). Temperature, pH and ligt intensity were monitored during the study.
Reference
Table - Measured Concentrations of TCPP in Freshwater Algal Medium Samples
Nominal Test Concentration (mg/L) | Sampling Time (Day) | Measured Concentration (mg/L)^ | Percent of Nominal | Mean Measured Concentration (mg/L) | Mean Measured Percent of Nominal |
0 | 0 3 | <LOQ <LOQ | - - | - | - |
6.3 | 0 3 | 6.64 6.29 | 105 99.8 | 6.5 | 103 |
13 | 0 3 | 13.8 13.0 | 106 100 | 13 | 100 |
25 | 0 3* 3 | 25.9 8.95 25.0 | 104 35.8 100 | 25 | 100 |
50 | 0 3 | 48.8 43.1 | 97.6 86.1 | 46 | 92 |
100 | 0 3 | 96.0 80.0 | 96.0 80.0 | 88 | 88 |
200 | 0 3 | 197 203 | 98.6 101 | 200 | 100 |
^ Limit of Quantification (LOQ)
* Due to a sampling error, sample reanalyzed and not included in calculation
Table - Mean Growth Rate and % Inhibition
Nominal Test Concentration (mg/L) | 0-24h | 0-48h | 0-72h | |||
Mean Growth Rate | Percent Inhibition | Mean Growth Rate | Percent Inhibition | Mean Growth Rate | Percent Inhibition | |
0 | 0.0450 | - | 0.0646 | - | 0.0674 | - |
6.3 | 0.0432 | 4.0 | 0.0623 | 3.6 | 0.0738 | -9.5 |
13 | 0.0288 | 36 | 0.0586 | 9.4 | 0.0679 | -0.68 |
25 | 0.0387 | 14 | 0.0560 | 13 | 0.0618* | 8.3 |
50 | 0.0212 | 53 | 0.0465 | 28 | 0.0518* | 23 |
100 | 0.0000 | 100 | 0.0237 | 63 | 0.0267* | 90 |
200 | 0.0000 | 100 | 0.0022 | 97 | 0.0023* | 67 |
Table - EC Values over the 72-Hour Growth Period
Time | Growth Rate | ||
ErC10 (95 % Confidence Interval) | ErC20 (95 % Confidence Interval) | ErC50 (95 % Confidence Interval) | |
24 hours | NA | NA | 47 mg/L (35 -65 mg/L) |
48 hours | NA | NA | 81 mg/L (74 - 88 mg/L) |
72 hours | 42 mg/L (36 - 50 mg/L) | 53 mg/L (46 - 61 mg/L) | 82 mg/L (76 - 89 mg/L) |
Description of key information
OECD 201 (Desjardins, 2004): 72h ErC50 = 82 mg/L (meas.), 72h ECrC10 = 42 mg/L (meas.)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 82 mg/L
- EC10 or NOEC for freshwater algae:
- 42 mg/L
Additional information
A single key study is submitted for this endpoint, a study on toxicity to the freshwater algae Pseudokirchnerella subcapitata of TCPP performed according to OECD Guideline 201 (Alga, Growth Inhibition Test) with analytical monitoring (Desjardins, 2004). The test gave an EC50 and an EC10 value for effects on growth of 82 mg/L and 42 mg/l, respectively and a NOEC of 13 mg/l after a test duration of 72 hours, relating to nominal concentrations as the test concentrations were analytically confirmed (Desjardins, 2004). Temperature, pH and ligt intensity were monitored during the study.
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