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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
6-chloro-N-ethyl-4-methyl-4-phenyl-4H-3,1-benzoxazin-2-amine
EC Number:
630-330-6
Cas Number:
21715-46-8
Molecular formula:
C17H18Cl2N2O
IUPAC Name:
6-chloro-N-ethyl-4-methyl-4-phenyl-4H-3,1-benzoxazin-2-amine
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Remarks:
test medium
Details on test solutions:
According to the sponsor, the test item was not soluble in the test medium, a special solubilization protocol was carried out before th eexperimentation, according to the OECD 23 guidance document on aquatic toxicity testing of difficult substances and mixtures.

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
22.5 - 25.1 °C
pH:
7.6 - 8.6
Details on test conditions:
Shaking at 120 rpm
Light intensity : 5927 lux
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
NOELR
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Key result
Duration:
72 h
Dose descriptor:
LOELR
Effect conc.:
3.2 mg/L
Nominal / measured:
nominal
Key result
Duration:
72 h
Dose descriptor:
EL50
Remarks:
growth rate
Effect conc.:
> 1.8 - < 4.37 mg/L
Nominal / measured:
nominal
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EL10
Remarks:
growth rate
Effect conc.:
0.31 mg/L
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EL50
Remarks:
Yield
Effect conc.:
> 1 - < 4.1 mg/L
Nominal / measured:
nominal
Basis for effect:
other: yield
Key result
Duration:
72 h
Dose descriptor:
EL10
Remarks:
Yield
Effect conc.:
0.3 mg/L
Nominal / measured:
nominal
Basis for effect:
other: yield
Details on results:
All the validity cruteria were successfully validated

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The effect of Etofixine on the growth of unicellular algae was performed according to the OECD 201 guideline. In test test conditions, the results obtained are :
- EL50 (growth rate) = 2.72 mg/L [1.80 - 4.37]
- EL50 (yield) = 1.95 mg/L [1.00 - 4.10]
Executive summary:

The effect of Etofixine on the growth of unicellular algae was performed according to the OECD 201 guideline. In test test conditions, the results obtained are :

- EL50 (growth rate) = 2.72 mg/L [1.80 - 4.37]

- EL50 (yield) = 1.95 mg/L [1.00 - 4.10]