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EC number: 237-857-7 | CAS number: 14024-56-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- Regulatory Requirements:
In deviation to the Study Plan, also the Toxic Substances Control Act (TSCA, 1975) was included in the Regulatory Requirements. This deviation did not have any impact on test results.
Experimental conditions
Minimum temperature in the negative control tank (min temperature 12.9°C) and in test item solutions tanks at 4.3 mg/L (min temperature 12.9°C), 9.4 mg/L (min temperature 12.0°C), 20.7 mg/L (min temperature 12.1°C) and 45.5 mg/L (min temperature 13.1°C) was lower than the OECD recommended range corrected for probe precision (13.5 – 16.5°C); this deviation was considered acceptable and without influence on test results, since neither sublethal effects nor lethal effects were observed during the test period.
Maximum temperature (16.9°C) in test item solution tank at 100.0 mg/L was higher than the OECD recommended range corrected for probe precision (13.5 – 16.5°C); this deviation was considered acceptable and without influence on test results, since it occurred only for a very short period of time (the first 5 minutes of the exposure period) and the gap to accepted temperature was very narrow (0.4°C).
Test item
EEC number is 628-792-9, as declared by the Sponsor. This deviation did not have any impact on test results. - Deviations:
- yes
- Remarks:
- See in version/remarks above.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Bis(pentane-2,4-dionato-O,O')magnesium
- EC Number:
- 237-857-7
- EC Name:
- Bis(pentane-2,4-dionato-O,O')magnesium
- Cas Number:
- 14024-56-7
- Molecular formula:
- C10H14MgO4
- IUPAC Name:
- magnesium;4-oxopent-2-en-2-olate
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of each test solution were taken, immediately after preparation at time 0, at 48 hours renewal and at the end of test (96 hours). Negative control samples were analysed at the same time to verify the absence of test item contamination.
Samples were labelled and analysed immediately after sampling.
Duplicate samples of the test water and the negative control were taken from the test solutions; one sample was analysed, while the respective duplicates were stored as retain samples to be analysed only if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test concentrations were chosen in accordance with the results of a no GLP pre-test (range-finding test), whose relevant Raw Data were archived under the code of the present study.
The test was performed at five test item concentrations in a geometric series (differing by a constant equal to 2.2), namely 4.3, 9.4, 20.7, 45.5 and 100.0 mg/L of test item.
The test medium was prepared as follows:
at the test start, 25 litres of a 100.0 mg test item/L stock solution were prepared by direct weighing into reconstituted water both at time 0 and at 48 hours renewal;
The obtained solutions were manually vigorously mixed until complete solubilization of the test item; thereafter the 5 flasks were merged together in the test tank and the other test concentrations were prepared by dilution with reconstituted water as described below
The obtained solutions were transparent and clear.
A negative control with reconstituted water only was also tested.
test solutions preparation:
Test solution 1:
-nominal test item solution concentration : 4.3mg/L
-final volume of tank : 15000mL
-added volume of test item stock solution 100.0 mg/L: 640mL
Test solution 2:
-nominal test item solution concentration : 9.4mg/L
-final volume of tank: 15000mL
-added volume of test item stock solution 100.0 mg/L: 1410mL
Test solution 3:
-nominal test item solution concentration : 20.7mg/L
-final volume of tank: 15000mL
-added volume of test item stock solution 100.0 mg/L: 3100mL
Test solution 4:
-nominal test item solution concentration : 45.5mg/L
-final volume of tank : 15000mL
-added volume of test item stock solution 100.0 mg/L: 6820mL
This procedure was repeated for the renewal of test solutions at 48 hours.
The test solutions and negative control were let reach the correct temperature and pH and dissolved oxygen concentration were measured before adding the fish.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- The study was performed with rainbow trout (Oncorhynchus mykiss) obtained from the Research Foundation “Edmund Mach” (S. Michele all’Adige - Italy). Juvenile fish were used and all of them were approximately the same age and size. Prior to the start of the test, the body weight and length of a representative number of fish (at least ten) was determined, recorded and reported in the Raw Data and in the Final Report. The mean body length was 4.7 cm.
In accordance with the test guidelines, the fish were held in the Test Facility laboratories for at least 12 days (13 days) in the same conditions, as for water quality, temperature and illumination, of the test. During the holding period, the fish were daily fed with specific fish food. Feeding was stopped at least 24 hours before the test start.
Study design
- Test type:
- semi-static
- Water media type:
- other: saltwater and freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 10 - 250 mg/L as CaCO3
- Test temperature:
- The temperature range provided by OECD No. 203 (1992) is 15.0 ± 2.0 °C, this range was corrected according to the probe precision, therefore the range to be considered for the test was 13.5 – 16.5 °C. Each temperature value was corrected according to the correction factor.
- pH:
- pH values were measured with a portable pHmeter and they did not vary more than 1.34 units throughout the test period.
- Dissolved oxygen:
- The dissolved oxygen concentrations were measured with a portable oxymeter and they were always above 60 % of the air saturation value, according to OECD No 203 (1992).
- Conductivity:
- < 10 μS/cm)
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The Short-term toxicity to fish test was performed according to the OECD Guideline 203 (Fish, Acute Toxicity Test).
96h EC0, EC50, EC100 and NOEC were respectively:
100mg/L, >100mg/L, >100mg/L and 100mg/L in Oncorhynchus mykiss.
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