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EC number: 619-946-6 | CAS number: 890707-29-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 9.4.2009 - 29.4.2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1200 (Acute Dermal Toxicity)
- Version / remarks:
- 1998
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Version / remarks:
- 1987
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: MAFF Japan Agricultural Chemicals Regulation Laws Notification 12 Nousan 8147
- Version / remarks:
- 2000
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 2-Amino-5-cyano-N,3-dimethylbenzamide
- Cas Number:
- 890707-29-6
- Molecular formula:
- C10H11N3O
- IUPAC Name:
- 2-Amino-5-cyano-N,3-dimethylbenzamide
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crl:CD(SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories, Raleigh, North Carolina
- Age at study initiation: Approximately 9 weeks old
- Weight at study initiation: 297.1–336.0 g for males; 204.2–239.6 g for females
- Housing: Animals were housed singly in polycarbonate pans that contained bedding and enrichment (e.g., nestlet or nylabone).
- Diet: PMI Nutrition International, LLC Certified Rodent LabDiet (#5002), ad libitum
- Water: Tap water, ad libitum
- Acclimation period: At least 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18-26
- Humidity (%): 30-70
- Air changes (per hr): Not recorded
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Type of coverage:
- semiocclusive
- Details on dermal exposure:
- Approximately 24 hours before dosing, the fur of each animal was closely shaved to expose the back from the scapular to the lumbar region (approximately 10% of each animal’s body surface area). A single dose of the test substance, moistened with approximately 0.5 mL of deionized water, was applied to the intact skin of 5 males and 5 females. The application site was covered with a porous gauze dressing. After 24 hours, excess test substance was washed from the dorsal skin of each animal with warm water and the skin was dried with a paper towel.
- Duration of exposure:
- 24 h
- Doses:
- - 5000 mg/kg bw
- No. of animals per sex per dose:
- 5 male and 5 female
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
Animals were observed for mortality and signs of illness, injury, or abnormal behavior daily. Observations for clinical signs and dermal irritation were made daily (weekends excluded). Dermal effects were scored according to the Draize Scale. The animals were weighed on test Days 0, 7 and 14.
- Necropsy of survivors performed: yes
All animals were euthanised and necropsied to detect grossly observable evidence of organ or tissue damage. - Statistics:
- The data did not warrant statistical analysis.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortalities occurred.
- Clinical signs:
- other:
- Body weight:
- other body weight observations
- Remarks:
- There were no test substance-related body weight effects noted.
- Gross pathology:
- One gross lesion was present in one rat. The gross lesion observed was skin ulcer/erosion on the neck. No other gross lesions were observed.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under conditions of this study, the dermal LD50 for the test substance was greater than 5000 mg/kg body weight when applied to the skin of male and female rats for 24 hours.
- Executive summary:
The acute dermal toxicity of the test substance was investigated according to OECD Guideline 402.
A single dose of the test substance, moistened with approximately 0.5 mL of deionized water, was applied to the shaved, intact skin of 5 male and 5 female rats at a dose of 5000 mg/kg body weight. The application site covered approximately 10% of each animal’s body surface area. The application site was semi-occluded for 24 hours after which the test substance was removed. The rats were observed for clinical signs, body weight effects, dermal effects, and mortality for 14 days following application. All animals were examined for gross pathological changes.
No mortalities were observed. The rats exhibited no clinical signs of systemic toxicity during the study. There were no test substance-related body weight effects noted. One gross lesion was present one rat. The gross lesion observed was skin ulcer/erosion on the neck. No other gross lesions were observed.
Under the conditions of this study, the skin absorption LD50 for the test substance was greater than 5000 mg/kg of body weight when applied to the skin of male and female rats for 24 hours.
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