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EC number: 217-691-1 | CAS number: 1931-62-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 September - 5 October 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- July 22, 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: M-161001 (according to CoA from 5 September 2017)
- Expiration date of the lot/batch: 2017-10-07
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Keep only in the original container. Protect from heat and direct sunlight. Store at max. +30°C
- Stability under test conditions: Not stable - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- A weight of 103.3 – 103.8 mg of the test item was added directly into the test vessels 7 - 11 with a test volume of 500 mL to obtain a final concentration of 99.9 – 100.4 mg/L. The pH of the test solution was measured before addition of inoculum.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from the municipal wastewater treatment plant Staufener Bucht was used as test system. The treatment plant has a capacity of 140,000 inhabitant equivalents and clarifies predominantly domestic wastewater. Sampling date of activated sludge was 4 October 2017. The activated sludge was kept aerated until use at test temperature. The activated sludge was washed twice by settling the sludge, decanting the supernatant and re-suspending the sludge in chlorine-free tap water. Dry solid of the activated sludge was determined as 4.5 g/L by weight measurements before and after drying at 105°C (mean of triplicate measurements). Before using, the activated sludge was diluted to 3 g/L dry solids with chlorine-free tap water, in order to obtain a final concentration of 1.5 g/L dry solids in the test.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- The temperature range within the test duration was between 20.4 and 20.7°C.
- pH:
- The pH in the test vessels was 6.9 and was adjusted to 7.2–7.3.
The pH in the blank vessels was 7.3. - Nominal and measured concentrations:
- Nominal test item concentration: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL glass beakers (Schott, Mainz, Germany))/250-300 mL BOD bottles
- Type (delete if not applicable): open
- Aeration: All vessels were stirred and aerated throughout the contact time with room air to assure an oxygen saturation of 60-70% using a membrane pump. Before starting the test the oxygen saturation rate was measured.
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 3
- Sludge concentration (weight of dry solids per volume): A final concentration of 1.5 g d.w./L was used in the test.
- Nutrients provided for bacteria: Synthetic sewage feed
- Nitrification inhibitor used (delete if not applicable): none
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: chlorine-free tap water
OTHER TEST CONDITIONS
- Adjustment of pH: yes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
After 3 h the aeration of the first vessel was shut off and the respiration rate was measured in a BOD-bottle with an oxygen electrode while mixing with a magnetic stirrer.
This determination was repeated for each vessel at 15-min intervals, ensuring that the contact time in each vessel was 3 h. - Reference substance (positive control):
- yes
- Remarks:
- 3-5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: The 3-hour EC50 of 3,5-dichlorphenol was calculated as 9.24 mg/L and was therefore within the acceptable range of 2 to 25 mg/L. - Reported statistics and error estimates:
- The EC50 value is calculated with the ToxRat software by probit analysis using linear maximum likelihood regression.
- Validity criteria fulfilled:
- yes
- Conclusions:
- An OECD 209 Activated sludge inhibition test (2010) was performed with OO-tert-butyl monoperoxymaleate at a limit concentration of 100 mg/L after a contact time of 3 hours.
An EC50 > 100 mg/L, a LOEC < 100 mg/L and a NOEC ≥ 100 mg/L were determined. - Executive summary:
To investigate potential harmful effects of OO-tert-butyl monoperoxymaleate to microorganisms, an activated sludge respiration inhibition test was performed according to OECD 209 and under GLP.
The study was conducted to assess the inhibitory effect of the test item at a limit concentration of 100 mg/L to microorganisms of activated sludge by measuring the respiration rate after adding a standard amount of synthetic sewage feed.
The results for the inhibition of the total respiration of activated sludge after the exposure period of 3 hours were:
EC50 > 100 mg/L
LOEC > 100.0 mg/L
NOEC >= 100.0 mg/L
Consequently, OO-tert-butyl monoperoxymaleate is not considered to elicit any adverse effects against STP organisms that would identify a relevant hazard.
The test met all guideline validity criteria.
The coefficient of variation (CV) of the blank respiration rates was less than 30% (presently 18%).
The blank controls mean oxygen uptake rate was higher than 20 mg oxygen per one gram of activated sludge per hour (presently 24.5 mg/g*h).
The 3-hour EC50 of the reference item was within the acceptable range of 2 to 25 mg/L (presently 9.24 mg/L).
Reference
Table 1: Respiration rate of activated sludge after application of the test item
No. |
Vessel |
O2 concentration [mg/L] |
Consumption rate |
Consumption rate |
inhibition |
|
first measurement |
last measurement |
|||||
1 |
Blank 1 |
7.04 |
5.19 |
44.4 |
29.6 |
|
2 |
Blank 2 |
7.43 |
5.64 |
43 |
28.6 |
|
3 |
Blank 3 |
7.86 |
6.85 |
24.2 |
16.2 |
|
17 |
Blank 4 |
7.39 |
5.89 |
36 |
24 |
|
18 |
Blank 5 |
7.43 |
5.93 |
36 |
24 |
|
19 |
Blank 6 |
8.17 |
6.63 |
37 |
24.6 |
|
7 |
test item 1 (100 mg/L) |
7.23 |
5.46 |
42.5 |
28.3 |
-16 |
8 |
test item 2 (100 mg/L) |
7.42 |
6.09 |
31.9 |
21.3 |
13 |
9 |
test item 3 (100 mg/L) |
8.25 |
7.09 |
27.8 |
18.6 |
24 |
10 |
test item 4 (100 mg/L) |
6.22 |
4.22 |
48 |
32 |
-31 |
11 |
test item 5 (100 mg/L) |
6.39 |
4.77 |
38.9 |
25.9 |
-6 |
coefficient of variation of blanks: |
0.18 |
|||||
mean consumption rate blanks: |
24.5 |
|||||
mean consumption rate test vessels: |
25.2 |
Description of key information
An OECD 209 activated sludge respiration inhibition test (2010) was performed with OO-tert-butyl monoperoxymaleate. The study was conducted to assess the inhibitory effect of the test item at a limit concentration of 100 mg/L to microorganisms of activated sludge by measuring the respiration rate after adding a standard amount of synthetic sewage feed. An EC50 > 100 mg/L, a LOEC < 100 mg/L and a NOEC ≥ 100 mg/L were determined.
This shows that OO-tert-butyl monoperoxymaleate had no toxic effect on the total respiration rate of activated sludge microorganism at the concentration of 100 mg/L after the exposure period of 3 hours.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
In an activated sludge respiration inhibition study a NOEC ≥ 100 mg/L was determined. Therefore, OO-tert-butyl monoperoxymaleate is not considered to elicit any adverse effects against STP organisms that would identify a relevant hazard and may justify PNEC derivation for regulatory purposes.
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