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EC number: 500-344-6 | CAS number: 157627-94-6 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 - 08 Nov 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- (2010)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Rheinland Pfalz, Germany
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test substance was warmed up in a water bath at 39.6 °C. Container was then shaken. Direct addition by initial weight into the test flasks followed. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: municipal wastewater treatment plant of Mannheim, Germany
- Preparation of inoculum for exposure: after arrival of the activated sludge suspension in the test facility the suspension was sieved with a fine woven mesh (mesh size about 1 mm). Suspension was pre-aerated over night at room temperature. At the next day sludge suspension was washed once with drinking water and adjusted to 3 g/L dw
- Initial biomass concentration: 1.5 g/L dw - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21.2 - 21.5 °C
- pH:
- 7.4 - 7.5 (initial pH)
7.9 - 8.0 (pH at the end) - Dissolved oxygen:
- 7.2 - 8.0 mg/L (test start)
- Nominal and measured concentrations:
- 0 (control), 182, 364, 729, 1458, 2915 mg/L (nominal test concentrations)
0 (control), 62.5, 125, 250, 500 and 1000 mg/L (a.i.) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 L glas beakers
- Fill volume: 500 mL (16 mL 100-fold concentrated synthetic medium with either test substance or reference substance + 234 mL deionized water + 250 mL inoculum suspension)
- Aeration: after preparation of the vessels incubation started by aeration of the vessels with pressured air
- Sludge concentration: 1.5 g/L dw
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per positive control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Intervals of water quality measurement: temperature was measured 8 times during the incubation in a separate test vessel with deionized water
EFFECT PARAMETERS MEASURED: oxygen uptake was measured for a period of about 8 to 10 min. Due to foaming of the test substance solution in the test vessel during aeration, 25 μL of triisobutyl sulfate was added in all test vessels as antifoam agent - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol (1, 10, 100 mg/L)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 915 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 140 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 410 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid: yes
- Relevant effect levels: EC50 = 3.5 mg/L - Reported statistics and error estimates:
- O2 consumption rates were used for the determination of the ECx by the probit method with the software TOXRAT Professional 2.10.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 14 - 15 Mar 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Preparation of inoculum for exposure: before the batches were inoculated, the extinction of the bacterial preliminary culture solution was measured and this was diluted with test culture nutrient medium to an extinction of 0.15 approximately (original bacterial suspension: extinction 0.152 at 436 nm.
- Initial biomass concentration: extinction 0.15 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 17 h
- Remarks on exposure duration:
- approximately
- Test temperature:
- 21.7 °C
- pH:
- 7.2 - 8.3
- Nominal and measured concentrations:
- nominal: 1250, 2500, 3750, 5000, 7500, 10000 mg a.i./L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size: sterile 250 mL Erlenmeyer flasks, plugged with cotton wool
- No. of vessels per concentration (replicates): 3 parallels for each of the 6 test substance batches
- No. of vessels per control (replicates): 6 growth controls, 6 control batches, 1 pH control for each of the 6 test substance batches. - Reference substance (positive control):
- no
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 14 - 15 Oct 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Initial biomass concentration: extinction of 0.140 at a wave length of 436 nm
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Remarks on exposure duration:
- 16 +/- 1 hour
- Test temperature:
- 21.8 °C
- pH:
- before incubation: 5.6 - 6.2
after incubation: 6.8 - Nominal and measured concentrations:
- nominal content of active substance: 313, 626, 1251, 2502.5, 5005 and 10010 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 mL Erlenmeyer flasks filled with 100 mL test medium
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6 - Reference substance (positive control):
- no
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the Category Approach Justification provided in Section 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other:
- Remarks:
- Source, key, 157627-95-7, 2016
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 915 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other:
- Remarks:
- Source, key, 157627-95-7, 2016
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 140 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other:
- Remarks:
- Source, key, 157627-95-7, 2016
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 410 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other:
- Remarks:
- Source, key, 157627-95-7, 2016
Referenceopen allclose all
EC80 > 1000 mg/L based on active ingredient (2915 mg/L based on nominal effect concentration)
Due to technical problems the measured values of the oxygen consumption after 3 hours of incubation of blank control 2 were lost.
Concentration [mg a.s./L] |
Extinction mean values at 436 nm |
Inhibition (%) |
Control* |
1.660 |
-- |
1250 |
1.686 |
-1.6 |
2500 |
1.646 |
0.9 |
3750 |
1.687 |
-1.6 |
5000 |
1.656 |
0.3 |
7500 |
1.588 |
4.4 |
10000 |
1.572 |
5.4 |
*cell multiplication: 1.644
Test Concentration [mg a.s./L] |
Inhibition [%] |
313 |
-6.9 |
626 |
-7.3 |
1251 |
-4.7 |
2502.5 |
-2.9 |
5005 |
-0.9 |
10010 |
7.4 |
Description of key information
EC50 (3 h) activated sludge > 1000 mg/L a.i. (nominal, OECD 209)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
For the toxicity to microorganisms no studies of AES (C12-14, 1-2.5 EO) TEA (CAS 157627-94-6) are available. Therefore, a read-across approach from AES (C16-18 and C18-unsaturated, 1-2.5 EO) Na (CAS 157627-95-7), AES (C12-14, 1-2.5EO) Na (CAS 68891-38-3) and AES (C12-14, 1-2.5EO) MIPA (CAS 1187742-72-8) was applied.
The key study investigated the toxicity of AES (C16-18 and C18-unsaturated, 1-2.5 EO) Na (CAS 157627-95-7) to activated sludge microorganisms (BASF, 2016). This GLP study was performed according to OECD guideline 209 with activated sludge from a municipal wastewater treatment plant. The respiration inhibition of the microorganisms was measured after 3 hours incubation to five test item concentrations (62.5, 125, 250, 500 and 1000 mg/L a.i.). The EC50 (3h) was determined to be > 1000 mg/L a.i. (nominal).
Two further studies confirms the low effect on microorganisms. Both tests were performed following the procedures of DIN 38412, part 8 and GLP. Based on growth inhibition EC10 values > 10000 mg a.i./L (nominal) for Pseudomonas putida were observed in both tests after 16 hours (Sasol, 1993d and Sasol, 1999d).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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