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EC number: 611-044-0 | CAS number: 53784-84-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 Jan 2016 - 09 Apr 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- (1R,3R,5S,6R,8S,10S,11S,13R,15S,16R,18R,20S,21R,23S,25S,26S,28R,30S,31R,33S,35S,36S,38R,40S,41R,42R,43R,44R,45R,46R,47R,48R,49R,50R,51R,52R,53R,54R,55R,56R)-5,10,15,20,25,30,35,40-octakis(bromomethyl)-2,4,7,9,12,14,17,19,22,24,27,29,32,34,37,39-hexadecaoxanonacyclo[36.2.2.2³,⁶.2⁸,¹¹.2¹³,¹⁶.2¹⁸,²¹.2²³,²⁶.2²⁸,³¹.2³³,³⁶]hexapentacontane-41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56-hexadecol
- EC Number:
- 611-044-0
- Cas Number:
- 53784-84-2
- Molecular formula:
- C48H72Br8O32
- IUPAC Name:
- (1R,3R,5S,6R,8S,10S,11S,13R,15S,16R,18R,20S,21R,23S,25S,26S,28R,30S,31R,33S,35S,36S,38R,40S,41R,42R,43R,44R,45R,46R,47R,48R,49R,50R,51R,52R,53R,54R,55R,56R)-5,10,15,20,25,30,35,40-octakis(bromomethyl)-2,4,7,9,12,14,17,19,22,24,27,29,32,34,37,39-hexadecaoxanonacyclo[36.2.2.2³,⁶.2⁸,¹¹.2¹³,¹⁶.2¹⁸,²¹.2²³,²⁶.2²⁸,³¹.2³³,³⁶]hexapentacontane-41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56-hexadecol
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Appearance: off-white powder
- Storage condition of test material: At room temperature
Constituent 1
- Specific details on test material used for the study:
- Solubility in water: 5.4 mg/L (20°C)
Stability in water: yes
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Concentrations: 0, 4.6, 10, 22, 46 and 100 mg/L
Sampling method: Singular samples for possible analysis were taken from all test concentrations and the control according to the schedule below.
Frequency: at t=0 h and t=48 h
Volume: 2.0 mL from the approximate centre of the test vessels.
Storage: samples were stored in a freezer until analysis
The filter containing the undissolved residue was kept for possible analysis.
At the end of the exposure period, the replicates were pooled at each concentration before sampling.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: preparation of test solutions started with the highest test concentration of 100 mg/L applying 2 days of magnetic stirring to ensure maximum dissolution of the test item in the test medium. The resulting dispersion was filtered through a 0.45 μm membrane filter (Whatman; RC55) to remove the undissolved test item, which resulted in a clear and colourless saturated solution (SS). The lower test concentrations were prepared by subsequent dilutions of the SS in test medium. Note that weighing and formulation of test solutions were performed protected from light as much as possible and that the control group received a similar treatment (i.e. filtration).
- Controls: test medium without test item or other additives
- Evidence of undissolved material: no
Controls: test medium without test item or other additives.
Evidence of undissolved material: no, all final test solutions were clear and colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straus, 1820
- Source: in-house laboratory culture with a known history, least third generation, obtained by acyclical parthenogenesis under specified breeding conditions. Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
- Age at study initiation: young daphnids with an age of < 24 hours, from parental daphnids of more than two weeks old.
- Cultivation method: Each batch was started with newborn daphnids, by placing about 250 of them into 5 litres of medium in an all-glass culture vessel. Cultures were renewed after 7 days of cultivation half of the medium twice a week.
- Feeding during test: no
Breeding conditions:
- Temperature of the medium: 18-22°C
- Feeding: daily, a suspension of fresh water algae.
- Breeding medium same as test medium: no, M7
ACCLIMATION: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/L CaCO3
- Test temperature:
- Between 19.4 and 20.4°C throughout the 2nd final test
- pH:
- Between 8.0 and 8.2 throughout the 2nd final test
- Dissolved oxygen:
- Between 8.7 and 9.1 mg O2/L throughout the 2nd final test
- Nominal and measured concentrations:
- Based on the results of the combined limit/range-finding test the expected EC50 ranged between concentrations obtained in 10 and 100% of the SS.
Nominal concentrations: 4.6, 10, 22, 46 and 100 mg/L
Analysis of the samples showed that concentrations were 77 to 97% of initial. Consequently, average measured concentrations were calculated.
Mean measured concentrations (2nd final test): 0.10, 0.25, 0.57, 1.2 and 2.9 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL, all-glass, open, fill-volume: 80 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Light intensity: none; the study was performed in the dark
EFFECT PARAMETERS MEASURED: Mobility (including mortality) after 48 hours. In addition, dissolved oxygen and pH of test medium were measured at the beginning and at the end of the test. Temperature of the medium was measured and controlled continuously from the start of the test.
RANGE-FINDING STUDY
- Test concentrations: 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes, based on the result, the expected EC50 ranged between concentrations obtained in 10 and 100% of the SS. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (December 2015)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.65 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Details on results:
- The first final test was repeated due to the fact that a dose-response effect was absent and consequently no reliable EC50 could be calculated. Above shown results are from the second final test.
- No biological, behavioural or other abnormalities observed
- Effect concentrations exceeding solubility of substance in test medium: no
All test conditions were maintained within the limits prescribed by the study plan. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- 24h-EC50: 0.69, 95%CI (0.59, 1.2)
- 48h-EC50: 0.38, 95%CI (0.33, 0.43)
Any other information on results incl. tables
Table 1 Measured concentrations versus nominal concentrations
Broomdex (%SS) |
Measured concentration (mg/L) |
Average measured (mg/L) |
|
t=0h |
t=48h |
||
4.6 |
0.114 |
0.095 |
0.10 |
10 |
0.287 |
0.222 |
0.25 |
22 |
0.621 |
0.532 |
0.57 |
46 |
1.27 |
1.23 |
1.2 |
100 |
2.94 |
2.81 |
2.9 |
Table 2 Number of introduced daphnids and incidence of immobilityin the 2ndfinal test
Time (h) |
Replicate |
Measured concentration Broomdex (mg/L) |
|||||
Control |
0.10 |
0.25 |
0.57 |
1.2 |
2.9 |
||
0 |
A |
5 |
5 |
5 |
5 |
5 |
5 |
B |
5 |
5 |
5 |
5 |
5 |
5 |
|
C |
5 |
5 |
5 |
5 |
5 |
5 |
|
D |
5 |
5 |
5 |
5 |
5 |
5 |
|
Total introduced |
20 |
20 |
20 |
20 |
20 |
20 |
|
24 |
A |
0 |
0 |
0 |
0 |
0 |
0 |
B |
0 |
0 |
0 |
0 |
0 |
0 |
|
C |
0 |
0 |
0 |
0 |
0 |
0 |
|
D |
0 |
0 |
0 |
0 |
0 |
1 |
|
Total immobilised |
0 |
0 |
0 |
0 |
0 |
1 |
|
Effect % |
0 |
0 |
0 |
0 |
0 |
5 |
|
|
|
|
|
|
|
||
48 |
A |
0 |
0 |
1 |
3 |
4 |
4 |
B |
0 |
0 |
0 |
2 |
4 |
5 |
|
C |
0 |
0 |
1 |
3 |
3 |
4 |
|
D |
0 |
0 |
0 |
4 |
4 |
5 |
|
Total immobilised |
0 |
0 |
2 |
12 |
15 |
18 |
|
Effect % |
0 |
0 |
10 |
60 |
75 |
90 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- 1) In the control, no daphnids became immobilised or showed other signs of disease or stress. 2) The oxygen concentration at the end of the test was ≥3 mg/L in control and test vessels.
- Conclusions:
- The 24h-EC50 could not be determined since it was > 2.9 mg/L. The 48h-EC50 was 0.65 mg/L based on average measured concentrations (95% confidence interval between 0.48 and 0.88 mg/L).
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