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EC number: 213-924-6 | CAS number: 1067-12-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental phase: 2018-03-13 - 2018-04-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method)
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
Test material
- Reference substance name:
- Phosphinylidynetrimethanol
- EC Number:
- 213-924-6
- EC Name:
- Phosphinylidynetrimethanol
- Cas Number:
- 1067-12-5
- Molecular formula:
- C3H9O4P
- IUPAC Name:
- [bis(hydroxymethyl)phosphoryl]methanol
- Test material form:
- liquid: viscous
- Details on test material:
- yellowish liquid, viscous, turbid
CAS no.: 1067-12-5
Storage conditions: at +10 to +25°C, in the tightly closed original container and stored in a cool, dry and well-ventilated place, protected from heat and sunlight
Constituent 1
- Specific details on test material used for the study:
- STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at +10 to +25°C, in the tightly closed original container and stored in a cool, dry and well-ventilated place, protected from heat and sunlight.
Test animals
- Species:
- rat
- Strain:
- other: Rat (Rattus norvegicus) / CD / Crl: CD(SD)
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories,
Research Models and Services,
Germany GmbH
- Age at study initiation: Approx. 8 weeks
- Weight at study initiation: 180 - 216 g
- Housing: Granulated textured wood, cages were changed and cleaned twice a week, Periodic analysis of the bedding material for contaminants based on EPA/USA is conducted by LUFA-ITL
- Diet (e.g. ad libitum): Commercial diet, ssniff® R/M-H V1534, feeding was discontinued approx. 16 hours before administration; only tap water was then available ad libitum.
- Water (e.g. ad libitum): Drinking water in bottles was offered ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22°C ± 3°C
- Humidity (%): 55% ± 10%
- Photoperiod (hrs dark / hrs light): dark/light periods: 12 hours each.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- methylcellulose
- Remarks:
- 0.8% aqueous hydroxypropylmethylcellulose
- Details on oral exposure:
- Tris(hydroxymethyl)phosphine oxide (THPO) was suspended in 0.8% aqueous hydroxypropylmethylcellulose to the appropriate concentrations. 0.8% aqueous hydroxypropylmethylcellulose was chosen as vehicle as it is known not to produce toxic effects.
VEHICLE: - Concentration in vehicle: 300 and 2000 mg/kg b.w.
- Amount of vehicle (if gavage): 10 mL/kg b.w.
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: Started with 300 mg/kg b.w., according to the OECD/EC guidelines: - Doses:
- 300 and 2000 mg/kg b.w.
Started with 300 mg/kg b.w. - No. of animals per sex per dose:
- 2 dose level groups of 6 female animals each
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations were performed before and immediately, 5, 15, 30 and 60 min, as well as 3, 6 and 24 hours after administration
- Necropsy of survivors performed: yes, at the end of the experiments, all animals were sacrificed, dissected and inspected macroscopically. All gross pathological changes were recorded.
- Other examinations performed: changes of skin and fur, eyes and mucous membranes, respiratory and the circulatory, autonomic and central nervous system and somatomotor activity as well as behaviour pattern were observed at least once a day until all symptoms subsided, thereafter each working day. Attention was also paid to possible tremors, convulsions, salivation, diarrhoea, lethargy, sleep and coma.
Observations on prematurely deceased animals were made at least once daily to minimize loss of animals during the study. The time of death would have been recorded as precisely as possible. Individual body weights were recorded before administration of the test item and thereafter in weekly intervals up to the end of the study. Changes in weight were calculated and recorded. - Statistics:
- No statistical analysis could be performed (the method used is not intended to allow a calculation of a precise LD50 value).
Results and discussion
- Preliminary study:
- Due to the small number of animals used with this method, there is no need to perform a range finding test.
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No animal died prematurely
- Clinical signs:
- other: Under the present test conditions, single oral administrations of 300 or 2000 mg Tris(hydroxymethyl)phosphine oxide (THPO)/kg b.w. did not reveal any signs of toxicity.
- Gross pathology:
- No pathological changes were observed at necropsy.
- Other findings:
- no details given
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- LD50 (14d): > 2000 mg Tris(hydroxymethyl)phosphine oxide (THPO)/kg b.w.
- Executive summary:
In this GLP study the acute toxicity after a single oral administration to rats of the test item Tris(hydroxymethyl)phosphine oxide (THPO) was carried out according to OECD Guideline for the Testing of Chemicals No. 423 - Acute oral toxicity - Acute Toxic Class Method (17 December 2001) and EC No. 440/2008 method B.1 tris (Acute oral toxicity - Acute toxic class method, 30 May 2008). Under the present test conditions, the single oral administrations of 300 or 2000 mg THPO/kg b.w. did not reveal any signs of toxicity. All animals gained the expected body weight. No animal died prematurely. The LD50 value (14 days) was determined to be > 2000 mg THPO / kg b.w. According to the EC Regulation 1272/2008 and subsequent regulations, the test material does not require classification for acute oral toxicity. Also according to the Globally Harmonized Classification System (GHS) the test item requires no labelling (as LD50 > 2000 mg/kg b.w.).
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