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EC number: 613-782-9 | CAS number: 65355-32-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Study period:
- 1996-07-30 to 1996-09-03
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- unsuitable test system
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 1992
- Deviations:
- yes
- Remarks:
- Only 3 test substance concentrations were applied, instead of at least 5 according to the guideline. No LC50 was determined.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Test solutions were prepared by stirring for 24 hours and then Ultra-Turrax treatment for 60 sec. Undissolved parts were removed by filtration (7-12 µm).
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Name in test report: Zebrabärbling (Brachydanio rerio HAMILTON BUCHANAN)
- Source: Charles River
- Age at study initiation: approx. 3 months
- Length at study initiation: 2.5 - 3.5 cm - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 21.6 - 22.5°C
- pH:
- 7.8 - 8.2
- Dissolved oxygen:
- 7.7 - 9.2 mg/L (90.8 - 108.0 % of saturation)
1 extreme value: at 2.1 mg/L after 48 h 6.6 mg/L (77.9 % satur.) - Nominal and measured concentrations:
- Nominal Concentration: 0.4, 0.9 and 2.1 mg/L; no measured concentration
- Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria 300 x 135 x 200 mm
- Fill volume: 5 L
- Aeration: yes
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater according to appendix 1 of EU test guideline
OTHER TEST CONDITIONS
- Photoperiod:16 h light / 8 h dark
TEST CONCENTRATIONS
- nominal concentrations: 0.4, 0.9, 2.1 mg/L - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 0.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- other:
- Remarks:
- geometric mean LC0 and LC100
- Effect conc.:
- 0.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 2.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- At 0.4 mg/L 1 fish (10 %) died. When the test was repeated, all test animals survived.
- Validity criteria fulfilled:
- no
- Conclusions:
- The geometric mean of LC0 and LC100 was calculated to be 0.9 mg/L.
- Executive summary:
A test on acute toxicity to fish was conducted according to EU Guideline C.1.3. The test substance was applied at 3 concentrations instead of at least 5 as stipulated in the test guideline. Therefore, the test is considered to be not valid. Three test substance concentrations and one control were applied. The LC0 was determined to be 0.4 mg/L and the LC100 was determined to be 2.1 mg/L, respectively. The geometric mean of LC0 and LC100 was calculated to be 0.9 mg/L.
Reference
Description of key information
The geometric mean of LC0 and LC100 was calculated to be 0.9 mg/L. The short-term toxicity to fish study was disregarded due to major methodical deficiencies (reference 6.1.1-1).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.9 mg/L
Additional information
A test on acute toxicity to fish was conducted according to EU Guideline C.1.3. The test substance was applied at 3 concentrations instead of at least 5 as stipulated in the test guideline. Therefore, the test is considered to be not valid. Three test substance concentrations and one control were applied. The LC0 was determined to be 0.4 mg/L and the LC100 was determined to be 2.1 mg/L, respectively. The geometric mean of LC0 and LC100 was calculated to be 0.9 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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