Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 243-606-2 | CAS number: 20217-01-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May - October 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: CG272
- Expiration date of the lot/batch: 11. July 2018
- Purity test date: not stated
RADIOLABELLING INFORMATION (if applicable)
not applicable
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Stability under test conditions: assumed stable
- Solubility and stability of the test substance in the solvent/vehicle: solubility 68.3 mg/L in water, assumed stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: assumed not reactive
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
none - Analytical monitoring:
- yes
- Details on sampling:
- not stated
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The solubility in dilution water was tested before the start of the test. As solubility lies below
100 mg/L, the water-accommodated fraction was prepared for the test. This was done
by weighing the nominal load of 100 mg/L (real load 101.9 mg/L) adding the corresponding
amount of dilution water and shaking vigorously for 24 hours. The resulting solution was
filtrated through 0.45 μm PTFE filters. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species Daphnia magna
Variety STRAUS
Strain Berlin
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin
In-house breeding since 27. September 2007
Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for
the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical.
The husbandry is performed similar to the method described in the OECD guideline, following
SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“), version 12
from 02. Feb. 2015.
Vessels preserving glasses, nominal volume 2 L
Medium M4-Medium (recipe of ELENDT)
Food unicellular green algae (Desmodesmus subspicatus)
Medium renewal twice a week
Photo period 16/8 hours, using neon tubes
Temperature 20.0 2.0 °C - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
- Hardness:
- 250
- Test temperature:
- 20.4 – 22.4 °C
- pH:
- 7.7 - 8.0
- Dissolved oxygen:
- 8.5 - 9.0 mg/L
- Salinity:
- not stated
- Conductivity:
- not stated
- Nominal and measured concentrations:
- At the start of the test the measured concentration of the test item was between 60 % and
66 % of the nominal concentration and after 48 hours the measured concentration was
between 53 % and 55% of the nominal concentration. - Details on test conditions:
- Medium renewal none
Test vessels glass beakers, nominal volume 100 mL, tall shape
Replicates (Treatments) 4 vessels, each containing 50 ± 5 mL test solution and 5
Daphnia
Replicates (Blank control) 4 vessels, each containing 50 ± 5 mL dilution water and 5
Daphnia - Reference substance (positive control):
- yes
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- VALIDITY
Immobilisation in the blank controls was 0 %.
The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L.
The lowest concentration of dissolved oxygen at the end of the test was 8.5 mg/L.
The pH-value in the test solutions should not vary by more than 1.5 units during the
test.
The highest variation was 0.3 units. - Results with reference substance (positive control):
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a
current reference study. The 24h-EC50i value was determined as 1.6 mg/L. - Reported statistics and error estimates:
- The estimation of the biological results was accomplished using the software ToxRat®
Professional, version 3.1. The details of calculation are stated in attached documents: Statistical calculation
using ToxRat® Professional 3.2.1 - Validity criteria fulfilled:
- yes
- Conclusions:
- he following results were determined for the test item DENACOL EX-147 (species:
Daphnia magna).
48h-NOEC = 1.3 mg/L
48h-LOEC = 2.6 mg/L
24h-EC50 = 6.7 mg/L
48h-EC50 = 2.5 mg/L - Executive summary:
One valid experiment was performed.
The study was performed using 6 concentrations ranging from 1 to 46 mg/L nominal concentration.
For each test concentration, 20 Daphnia were exposed to the test item for 48
hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.
Four concentrations showed toxicity between 65 and 100% immobilisation after 48 hours.
None of the animals were immobilised in the blank control.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a
current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions
was determined using GC/FID-determination. At the start of the test the measured concentration
of the test item was between 60 % and 66% of the nominal concentration and after
48 hours the measured concentration was between 53 % and 55% of the nominal concentration.
Therefore, the determination of the biological results was based on the geometric
mean of measured concentrations.
The following results were determined for the test item DENACOL EX-147 (species:
Daphnia magna).
48h-NOEC = 1.3 mg/L
48h-LOEC = 2.6 mg/L
24h-EC50 = 6.7 mg/L
48h-EC50 = 2.5 mg/L
Reference
Description of key information
one valid Guideline Study available on toxicity towards Daphnia Magna
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.5 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.