Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-754-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Sublethal pH Decrease May Cause Genetic Damage to Eukaryotic Cell: A Study on Sea U rc h ins and Salmonella typhimurium
- Author:
- Maria Cipollaro, Giuliana Corsale, Agostino Esposito, Enrica Ragucci, Norma Staiano, Giovan Giacomo Giordano, and Giovanni Pagano
- Year:
- 1 986
- Bibliographic source:
- Teratogenesis, Carcinogenesis, and Mutagenesis 6:275-287 (1986)
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- pH changes on bacterial reversion rate was evaluated by adopting 2 modifications of the standard plate incorporation assay
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Orthophosphoric acid
- EC Number:
- 231-633-2
- EC Name:
- Orthophosphoric acid
- Cas Number:
- 7664-38-2
- Molecular formula:
- H3O4P
- IUPAC Name:
- phosphoric acid
Constituent 1
Method
- Target gene:
- Histidine locus
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 97
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- S. typhimurium TA 102
- Species / strain / cell type:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S9 was included but the study did not specify if it was used for this test
- Test concentrations with justification for top dose:
- up to toxic level, different pH’s (ranging from 4 to 9)
Controlsopen allclose all
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Positive controls:
- yes
- Positive control substance:
- other: daunomycin
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Additional information on results:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
The reversion properties and specificity of each strain were confirmed by testing MMS, daunomycin, and sodium azide in the standard plate-incorporation assay.
Applicant's summary and conclusion
- Conclusions:
- No effects were detectable in S. typhimurium tester strains following sublethal pH decrease.
- Executive summary:
The incubation of S. typhimurium tester strains with different buffer solutions at pH ranging from 5.5 to 9 had no effect on the bacterial reversion rates. The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed.
The same lack of effects was obtained by using the base agar plates at different pH values.
The ineffectiveness of pH decrease was invariably unchanged by the addition of S9 fraction, however no detailed results were reported.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.