Allyltrimethylsilane

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

A Compilation of Two Decades of Mutagenicity Test Results with the Ames Salmonella typhimurium and L5178Y Mouse Lymphoma Cell Mutation Assays

Author:

Seifried, H.E. et al.

Year:

2006

Bibliographic source:

Chem. Res. Toxicol. 19:627 - 644

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

The 5th strain is missing either E.coli or TA102

GLP compliance:

not specified

Type of assay:

bacterial forward mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Allyltrimethylsilane

EC Number:

212-104-5

EC Name:

Allyltrimethylsilane

Cas Number:

762-72-1

Molecular formula:

C6H14Si

IUPAC Name:

allyltrimethylsilane

Test material form:

liquid

Method

Target gene:

his operon

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

not specified

Species / strain 2

Species / strain / cell type:

S. typhimurium TA 1538

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

Aroclor 1254-induced rat liver S9 and induced hamster liver S9 mix

Test concentrations with justification for top dose:

1 to 1000 µg/plate based on a preliminary dose range-finding study using strain TA100.

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: not reported; however, the study states that the appropriate concurrent solvent was used

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

not specified

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)
- Cell density at seeding: (1-2) X 10^9 cells/mL

DURATION
- Exposure duration: 48 h

NUMBER OF REPLICATIONS: triplicate

Rationale for test conditions:

Not reported

Evaluation criteria:

For the test article to be considered positive, it had to induce at least a doubling (TA98, TA100, and TA1535) in the mean number of revertants per plate of at least one tester strain. This increase inthe mean revertants per plate had to be accompanied by a doseresponse to increasing concentrations of the test chemical. If the study showed a dose response with a less than 3-fold increase onTA1537 or TA1538, the response had to be confirmed in a repeat experiment

Statistics:

Not reported

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium, other: TA98, TA100, TA1535, TA1537, and TA1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not applicable

Positive controls validity:

not specified

Additional information on results:

No additional information

Applicant's summary and conclusion

Conclusions:

There was no evidence of a test-substance related increase in the number of revertants with or without activation.