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EC number: 220-278-9 | CAS number: 2698-41-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
- Reference Type:
- review article or handbook
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Version / remarks:
- Procedures described previously (King et al. 1981)
- Deviations:
- not specified
- GLP compliance:
- not specified
- Type of assay:
- mammalian erythrocyte micronucleus test
Test material
- Reference substance name:
- [(2-chlorophenyl)methylene]malononitrile
- EC Number:
- 220-278-9
- EC Name:
- [(2-chlorophenyl)methylene]malononitrile
- Cas Number:
- 2698-41-1
- Molecular formula:
- C10H5ClN2
- IUPAC Name:
- [(2-chlorophenyl)methylene]malononitrile
Constituent 1
- Specific details on test material used for the study:
- purity level not specified
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- not specified
Administration / exposure
- Route of administration:
- other: intraperitoneal and per os
- Vehicle:
- olive oil = negative control
- Duration of treatment / exposure:
- One administration
- Frequency of treatment:
- once
- Post exposure period:
- Expression time 30 to 48 hours
Doses / concentrationsopen allclose all
- Dose / conc.:
- 37.8 mg/kg bw (total dose)
- Remarks:
- intraperitoneal dose - 3/4 of the reported intraperitoneal LD50 in mice (NIOSH 1980)
- Dose / conc.:
- 18.9 mg/kg bw (total dose)
- Remarks:
- intraperitoneal dose - half of highest dose
- Dose / conc.:
- 226 mg/kg bw (total dose)
- Remarks:
- per os dose, equivalent to oral LD50 reported by NIOSH 1980
- Dose / conc.:
- 113 mg/kg bw (total dose)
- Remarks:
- half of highest dose per os
- No. of animals per sex per dose:
- 5 per dose, except for highest dose per os (3 mice survived among 13 as LD50)
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- procarbazine per os and ip
Examinations
- Tissues and cell types examined:
- bown marrow erythrocytes
- Details of tissue and slide preparation:
- point mutation, germinal gene mutations, chromosomal breaks and mitotic chromosome misdistribution
- Evaluation criteria:
- presence/absence
- Statistics:
- not specified
Results and discussion
Test results
- Key result
- Sex:
- not specified
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- expected as highest dose per os equivalent to LD50
- Vehicle controls validity:
- not specified
- Negative controls validity:
- valid
- Remarks:
- olive oil
- Positive controls validity:
- valid
- Additional information on results:
- the failure to observe any of these effects is compatible with the lack of DNA binding of CS and metabolites, in accordance with (von Daniken et al 1981) and (NTP tr377 report, 1990)
Applicant's summary and conclusion
- Conclusions:
- No genetoxicity (micronucleus) is observed with CS susbtance in mice
- Executive summary:
No genetoxicity is observed in micronucleus assay in mice (comparable to OECD 474 methodology) after CS exposure by intraperitoneal and per os routes.
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