(2,6-xylyloxy) acetic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: done under GLP and OECD method

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

430-910-7

EC Name:

-

Cas Number:

13335-71-2

Molecular formula:

Hill formula: C10H12O3 CAS formula: C10H12O3

IUPAC Name:

2-(2,6-dimethylphenoxy)acetic acid

Details on test material:

white light tan powder, purity = 99.8%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

samples were withdrawn from the treatment solutions at 0 and 48 h;

Test solutions

Vehicle:

no

Details on test solutions:

test solution prepared by transferring wighted amounts of A-172245.0 into the glass beakers. The containers were made up to 100 mL with reconstituted water in order to obtain the final concentration of 100 mg/l

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Fill in what you can

TEST ORGANISM
- Common name:
- Strain:
- Source:
- Age at study initiation (mean and range, SD):
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

The obersavation period was 48 hours. observation for immobilization was made at 24 and 48 hours. Daphnia was considered immobile if not able to swim within 15 seconds of gentle agitation of the test container.

Test conditions

Hardness:

200-250 mg/l

Test temperature:

temperature was monitored

pH:

pH was monitored

Nominal and measured concentrations:

Nominal concentration = 100 mg/l

measured conc = 102.17 mg/l @ 0 hours
measured conc = 101.63 mg/l @ 48 hours

Details on test conditions:

during the test, the animals were kept in the dark

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of A-172245.0 @24 hours and @ 48 hours was higher than 100mg/l