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EC number: 248-387-7 | CAS number: 27287-73-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 May 2016 to 14 June 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 425 (Acute Oral Toxicity: Up-and-Down Procedure)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- up-and-down procedure
- Limit test:
- no
Test material
- Reference substance name:
- 2-amino-6-methyl-1,2,4-triazolo[1,5-a]pyrimidin-5(1H)-one
- EC Number:
- 248-387-7
- EC Name:
- 2-amino-6-methyl-1,2,4-triazolo[1,5-a]pyrimidin-5(1H)-one
- Cas Number:
- 27287-73-6
- Molecular formula:
- C6H7N5O
- IUPAC Name:
- 2-amino-6-methyl-1,2,4-triazolo[1,5-a]pyrimidin-5(1H)-one
- Test material form:
- solid: particulate/powder
- Details on test material:
- -Storage conditions: room temperature in the dark
-Colour: beige
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crl:WI Wistar rats
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories, Research Models and Services, Germany GmbH, Sandhofer Weg 7, D-97633 Sulzfeld, Germany.
- Females (if applicable) nulliparous and non-pregnant: Yes
- Age at study initiation: 8 - 10 weeks old
- Weight at study initiation: 201 - 218g
- Fasting period before study: Overnight prior to dosing
- Housing: Animals were housed individually in Type II polypropylene/polycarbonate cages. Animals were housed with deep wood sawdust bedding to allow digging and other normal rodent activities.
- Diet (e.g. ad libitum): Yes
- Water (e.g. ad libitum): Yes
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19.9 - 25.0°C
- Humidity (%): 32 - 70%
- Air changes (per hr): 15 - 20 air changes per hour
- Photoperiod (hrs dark / hrs light): 12 hours light from 6:00am to 6:00pm
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 17.5 mg/L, 55 mg/L and 200 mg/L for 175, 500 and 2000 mg/kg bw doses respectively.
- Amount of vehicle (if gavage): 1% Carboxymethyl cellulose (CMC)
MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg bw
- Doses:
- 175, 550 and 2000 mg/kg body weight
- No. of animals per sex per dose:
- 175 mg/kg bw = 1 animal
550 mg/kg bw = 1 animal
2000 mg/kg bw = 3 animals - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: animals were observed for clinical signs 30 minutes after dosing, then at approximately 1, 2, 3, 4 and 6 hours after dosing and once each day for 14 days thereafter. Body weights were recorded on days -1, 0 (before treatment), 7 and 14.
- Necropsy of survivors performed: yes
- Other examinations performed: All animals were necropsied and subject to gross macroscopic examination. - Statistics:
- The LD50 was calculated using the AOT425StatPgm program. This program was prepared for the US Environmental Protection Agency by Westat, May 2001 and updated by the US EPA June 2003. This programme was constructed using the most appropriate method to estimate the LD50.
Results and discussion
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- not determinable due to absence of adverse toxic effects
- Mortality:
- There was no mortality during the study
- Clinical signs:
- At dose level 175 mg/kg bw, hunched back was observed only on the day of treatment in 1/1 animals.
At dose level 550 mg/kg bw, hunched back and decreased activity were observed only on the day of treatment in 1/1 animals.
At dose level 2000 mg/kg bw, hunched back in 3/3 animals, decreased activity in 2/3 animals, incoordination in 1/3 animals and piloerection in 1/3 animals on the day of treatment.
There were no further clinical signs after Day 0 at any dose level. - Body weight:
- There were no treatment related effects on body weight or body weight gain
- Gross pathology:
- At necropsy there were no observations to be reported at any dose level
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- An acute oral toxicity study was conducted with 5 female Crl:WI rats according to the OECD guideline 425. Under the conditions of this study, the estimated acute oral median lethal dose (LD50) of the test item was found to be greater than 2000 mg/kg bw in female Crl:WI rats.
- Executive summary:
An acute oral toxicity (up and down procedure) study was conducted with 5 female Crl:WI rats according to the OECD guideline 425.
Animals were treated with a single oral (gavage) dose of the test substance at dose levels of 175, 550 or 2000 mg/kg body weight (bw) followed by a 14-day observation period. The animals were fasted overnight prior to treatment and food was returned 3 hours after dosing. Animals were observed individually after dosing at 30 minutes, then 1, 2, 3, 4 and 6 hours post treatment and once each day for 14 days thereafter. Body weight was measured on Day -1, just before dosing and weekly thereafter. All animals were examined macroscopically at the end of the observation period.
There was no mortality during the study. At dose level 175 mg/kg bw, hunched back was observed only on the day of treatment in 1/1 animals. At dose level 550 mg/kg bw, hunched back and decreased activity were observed only on the day of treatment in 1/1 animals. At dose level 2000 mg/kg bw, hunched back in 3/3 animals, decreased activity in 2/3 animals, incoordination in 1/3 animals and piloerection in 1/3 animals on the day of treatment.
There were no further clinical signs after Day 0 at any dose level. There were no treatment related effects on body weight or body weight gain. Body weights were within the range commonly recorded for this strain and age. At necropsy, there were no observations to be reported at a dose level of 175, 550 or 2000 mg/kg bw.
Under the conditions of this study, the estimated acute oral median lethal dose (LD50) of the test item was found to be greater than 2000 mg/kg bw in female Crl:WI rats.
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