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EC number: 302-766-4 | CAS number: 94134-01-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02/02/2005 - 03/05/2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dissolved in seawater
- Final preparation of a solid: Dissolved in seawater
FORM AS APPLIED IN THE TEST (if different from that of starting material) Aqueous, in solution
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: Definitive test (nominal concentrations): 100, 320, 1000, 3200, 10 006 mg/L
- Sampling method: Growth rate of population - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test material dissolved in seawater
- Controls: 4 growth rate controls (1 replicate), 5 positive controls (Reference substance, 3,5-Dichlorophenol) at 5.6, 3.2, 1.8, 1.0 and 0.56 mg/L(2 replicates)
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No, test was soluble in seawater - Test organisms (species):
- Skeletonema costatum
- Details on test organisms:
- TEST ORGANISM
- Common name: Skeletonema costatum
- Strain: C5B1 A
- Source (laboratory, culture collection): Stock laboratory culture
- Method of cultivation: Inoculation of ISO culture medium
ACCLIMATION
- Any deformed or abnormal cells observed: No - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- Measurements were taken at 24, 48 and 72 hours
- Test temperature:
- 18.6 - 21.6
- pH:
- Control and Definitive test (0 hours) pH 7.82 - 8.15
Definitive test (1 hour) pH 8.12 - 9.09 - Conductivity:
- 52.1 - 53.4 mS/cm
- Nominal and measured concentrations:
- Nominal test substance concentrations- 100, 320, 1000, 3200, 10 006 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: conical flask
- Type: closed
- Material, size, headspace, fill volume: 100 ml
- Aeration: Flasks shaken (orbital shaker)
- Initial cells density: 10235 cells/ ml
- Control end cells density: 8.66 - 8.81
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): Growth rate control (1 replicate), positive control (2 replicates)
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural seawater, from Scapa Flow, Orkney.
- Alkalinity: pH 8 (+- 0.2)
- Particulate matter: 0.2 µm filtered seawater
- Culture medium different from test medium: No
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Sterile conditions: Yes
- Light intensity and quality: Cool white illumination
- Salinity (for marine algae): natural seawater
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : growth rate of population
- Determination of cell concentrations: microscope using either a haemocytometer or Sedgewick-Rafter chamber
TEST CONCENTRATIONS
- Range finding study: 1, 10, 100 and 1000 mg/ L test substance, no observed effect
- Test concentrations: Definitive test: 100, 320, 1000, 10 000 mg/ L
- Results used to determine the conditions for the definitive study: Results from Rangefinding test used - Reference substance (positive control):
- yes
- Remarks:
- 3,5 Dichlorophenol
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 842.82 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2 104.86 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 935.67 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 2 956.24 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): no
- Observation of abnormalities (for algal test): no
- Any stimulation of growth found in any treatment: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 2.86 mg L
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance has an EC50 of 1842.82 mg /L for 24 hours, 2104.86 mg/L for 48 hours and 1935.67 mg/L for 72 hours. The EC90 for 72 hours is 2956.24 mg/L and the NOEC for 72 hours is 320 mg/L.
- Executive summary:
The study assesses Monoethanolamine Salt of Nitrilotriacetic Acid's toxicity to aquatic algae. The study is GLP compliant and was performed according to the ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum). The test organism was Skeletonema costatum and all the validity criteria were met. A rangefinding test was performed for the test substance (nominal concentrations, 1, 10, 100, 1000 mg/L) however, there was no observed effect on the test organism growth rate over these concentrations. Monoethanolamine Salt of Nitrilotriacetic Acid exhibited a 72h EC50 value of 1935.67 mg/L (dilution series) to the marine phytoplankton Skeletonema costatum in the aqueous phase.
Reference
Description of key information
The study assesses Monoethanolamine Salt of Nitrilotriacetic Acid's toxicity to aquatic algae. The study is GLP compliant and was performed according to the ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum). The test organism was Skeletonema costatum and all the validity criteria were met. A rangefinding test was performed for the test substance (nominal concentrations, 1, 10, 100, 1000 mg/L) however, there was no observed effect on the test organism growth rate over these concentrations. Monoethanolamine Salt of Nitrilotriacetic Acid exhibited a 72h EC50 value of 1935.67 mg/L (dilution series) to the marine phytoplankton Skeletonema costatum in the aqueous phase.
Key value for chemical safety assessment
- EC50 for marine water algae:
- 1 935.67 mg/L
- EC10 or NOEC for marine water algae:
- 320 mg/L
Additional information
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