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EC number: 218-345-2 | CAS number: 2128-93-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 July to 04 August 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch No.of test material: 20161118
- Expiration date of the lot/batch: 17-11-2017
- Purity test date: 18-11-2016
- Purity: 99.74%
- Appearance: white to off white crystaline powder
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient, protected from light
- Stability under test conditions: No data
- Solubility and stability of the test substance in the solvent/vehicle: No data - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Waterschap Aa en Maas, s'Hertogenbosch, The Netherlands
- Preparation of inoculum for exposure: Used immediately after collection
- Pretreatment: Allowed to settle, supernatant liquid used as inoculum
- Concentration of sludge: 3.6 g/L suspended solids concentration
- Duration of test (contact time):
- >= 28 d
- Initial conc.:
- >= 13.5 mg/L
- Based on:
- other: Molecular formula
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
- Additional substrate:
- Solubilising agent (type and concentration if used):
- Test temperature:
- pH:
- pH adjusted: yes/no
- CEC (meq/100 g):
- Aeration of dilution water:
- Suspended solids concentration:
- Continuous darkness: yes/no
- Other:
TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used:
- Other:
SAMPLING
- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 test vessels
- Positive control: 1 test vessel
- Toxicity control: 1 test vessel
- Other:
- Reference substance:
- acetic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 1
- Sampling time:
- 6 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 1
- Sampling time:
- 15 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 1
- Sampling time:
- 22 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 2
- Sampling time:
- 28 d
- Remarks on result:
- other: Not readily biodegradable
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance was not readily biodegradable under the conditions of the test
- Executive summary:
A study was performed to assess the ready biodegradability of the test substance according to the OECD guideline OECD 301B (CO2 evolution test (modified Sturm test), in compliance with GLP.
The test substance was incubated with microbial organisms from a domestic effluent treatment works, in a mineral medium for 28 days. Relative biodegradation was calculated from CO2 evolution measurements determined over the course of the study.
The test substance showed 2% biodegradation after 28 days, and therefore, is classified as not readily biodegradable under the conditions of the test. The positive control showed 85% biodegradation after 14 days confirming the viability of the microbial inoculum. The toxicity control showed 41% biodegradation after 14 days confirming the test substance was not inhibitory to microbial activity.
All acceptability criteria were met, therefore, this study was considered to be valid.
In conclusion, under the test conditions, the test substance was considered to be not readily biodegradable.
However, it should be noted that this study was run at a concentration of 13.5mg/L greater than the limit of solubility (0.0736mg/L) and it is unlikely that the test substance would have been available to the microbial population for biodegradation.
Reference
Time (days) |
Cumulative CO2production (mg) |
Biodegradation (%) |
||||||
Inoculum blank1 |
Test item1 |
Positive control (Sodium acetate) |
Toxicity control (Test item & sodium acetate) |
Inoculum blank1 |
Test item1 |
Positive control (Sodium acetate) |
Toxicity control (Test item & sodium acetate) |
|
1 |
2.6 |
0.0 |
0.0 |
0.0 |
- |
0 |
0 |
0 |
4 |
7.0 |
0.0 |
21.7 |
24.9 |
- |
0 |
25 |
14 |
6 |
10.7 |
0.6 |
37.6 |
42.7 |
- |
1 |
44 |
25 |
8 |
15.2 |
0.6 |
48.3 |
52.9 |
- |
1 |
56 |
31 |
11 |
19.6 |
0.7 |
59.0 |
60.9 |
- |
1 |
69 |
35 |
15 |
24.1 |
0.8 |
72.8 |
71.6 |
- |
1 |
85 |
41 |
18 |
28.6 |
1.0 |
- |
- |
- |
1 |
- |
- |
22 |
33.7 |
1.2 |
- |
- |
- |
1 |
- |
- |
25 |
38.7 |
1.4 |
- |
- |
- |
1 |
- |
- |
29 |
48.1* |
1.6* |
- |
- |
- |
2* |
- |
- |
Inoculum blank and test item: Microbial activity ended on day 28 by addition of HCl, but measurements taken day 29
Positive and toxicity control: Microbial activity ended on day 14 by addition of HCl, but measurements taken day 15
1 Mean of 2 test vessels
* Mean of 3 determinations
Description of key information
A reliable study (Klimisch 1) assessed ready biodegradability using a standard method in compliance with OECD guideline 301B, according to GLP.
After 28 days a maximum of 2% degradation was observed, therefore, the test substance should be considered as not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The test substance in a mineral medium was inoculated with secondary domestic effluent and incubated for 28 days under aerobic conditions. Biodegradation was calculated from CO2 evolution measurements determined over the course of the study. The test substance showed a maximum of 2 % degradation after 28 days. Therefore, the test substance should be considered as not readily biodegradable.
However, it should be noted that this study was run at a concentration of 13.5mg/L, based on molecular formula and is considered to be greatly over the limit of solubility (0.0736mg/L). Due to this, it is unlikely that the test substance would have been bioavailable for biodegradation by the microbes.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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