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EC number: 235-517-2 | CAS number: 12262-25-8 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 53430.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- For justification, please refer to Read Across Statement in IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
Reference
Description of key information
In an Activated Sludge Respiration Inhibition Test with the read across substance (CAS No 1040873-93-5) according to OECD guideline 209, the 3-h EC50 and NOEC was determined to be > 1000 mg/L and 1000 mg/L, respectively.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
No data on toxicity to aquatic microorganisms were available for the target substance (CAS No 12262-25 -8) itself. Therefore, a read across to the structural analogue read across substance (CAS No 1040873-93-5) was conducted.
To evaluate the influence of the structural analogue read across substance on the activity of activated sludge, a 3-hour Activated Sludge Respiration Inhibition Test according to OECD Guideline 209, EU Method C.11 and US EPA OCSPP 850.3300 was conducted. In a preliminary test, the respiration rates (total, heterotrophic and nitrification oxygen uptake rates) of samples of activated sludge fed with synthetic sewage were measured in an enclosed cell containing an oxygen electrode after a contact time of 3 hours. The structural analogue was investigated in this study at the nominal concentrations of 10, 100 and 1000 mg/L. Defined amounts of the ground test item were added directly into the test vessels. Triplicate vessels were prepared and investigated at the highest examined test item concentration. In parallel with the test item treatments 3,5-Dichlorophenol as positive reference control in a concentrations of 2, 7 and 24.5 mg/L; furthermore, blank (inoculum) control, nitrification controls and abiotic controls were investigated. Abiotic controls (investigated in three parallels) were prepared containing the test item in the concentration of 1000 mg/L, synthetics sewage feed, but no inoculum. In this test no abiotic oxygen consumption was noticed. The test was performed without pH adjustment. During the test, significant effect of the test item on the pH was realized at the highest examined concentration of 1000 mg/L. The pH of the test mixtures in presence of 1000 mg/L (the highest examined concentration) test item was ~5 without inoculum, but within the 7—8 pH range after inoculation.The test item may adversely affect pH within the test system; however the results of the present preliminary test show, that the slightly lower pH caused by the test item has no effect on the applied inoculum culture and further testing (a definite test) with additional neutralization step of test item containing mixtures before inoculum addition is not necessary. All validity criteria of the study were met. The average specific respiration rate of the blank was 33.69 mg O2/ g activated sludge (based on dry weight) in an hour with a coefficient of variation of 2.87 %. The 3-hour EC50 of the reference item 3,5-Dichlorophenol was 13.87 mg/L within the range of 2 mg/L to 25 mg/L, that was required for total respiration (in this study thedifferentiation between heterotrophic respiration and nitrification was considered as not necessary). The observed oxygen consumption rates and consequently the specific respiration rates in all examined test item concentrations remained in the range of the blank controls (the average specific respiration rate at 1000 mg/L: 32.87 mg O2/gh). No inhibitory effect of the test item was observed.
Based on measured oxygen consumption values and calculated specific respiration rates it can be stated that the 3-hour EC10 and EC50 values of the test item are greater than 1000 mg/L. The NOEC was determined to be 1000 mg/L, the highest concentration tested. The specific respiration rates at the examined highest test item concentration of 1000 mg/L were compared with the blank controlvalues using 2-Sample T-test (a=0.05). No statistical significant differences were observed compared to the blank control values. In conclusion, this preliminary test demonstrated the absence of inhibition of oxygen consumption by the test substance up to and including the limit concentration of 1000 mg/L, therefore in line with OECD 209 a definite test is not required.
Based on the results of the preliminary test with the source substance (CAS No 1040873-93-5), the EC50 and EC10 of the target substance (CAS No 12262-25 -8) was determined to be > 1000 mg/L and the NOEC to be 1000 mg/L.
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