Butyl ricinoleate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 March 2018 to 16 March 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

2008

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

The test substance is listed as Butyl Ricinoleate (CAS 151-13-3). The test substance consists of an off-white solid and was obtained from the Sponsor, Vertellus Holdings LLC and stored at ambient conditions.

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

The test was carried out using Daphnia magna neonates (<24 hours old) obtained from an in-house daphnid culture.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

19.9 °C to 20.7 °C

pH:

8.4 to 8.5

Dissolved oxygen:

6.9 to 8.8 mg O2/L (81% to 101% saturation)

Nominal and measured concentrations:

Following a preliminary range-finding test, a definitive limit test with a target nominal loading rate concentration range of 0 (control) and 100 mg Total Product (TP)/L was conducted.

Details on test conditions:

Following preliminary range-finding tests, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item with a WAF nominal concentration of 100 mg TP/L solution for 48 hours at a temperature of 19.9 °C to 20.7 °C under static test conditions

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Remarks:

None of the control daphnids showed immobilization or other signs of disease or stress and the oxygen concentration at the end of the test was > 7.9 mg/L which is greater than the acceptable requirement of > 3 mg/L in the control and test vessels.

Conclusions:

The test substance did not induce acute immobilisation of Daphnia magna at the nominal concentration limit test after 48 h of exposure. The effects concentrations were based on the nominal test concentration of 100 mg Total Product (TP)/L. The 24- and 48-hour EC50 values were estimated to be >100 mg TP/L. No sublethal effects were noted during the definitive test. The 48 hour NOEC was 100 mg TP/L.

Description of key information

The key study was a static acute invertebrate toxicity test on the effects of the substance on the freshwater invertebrate Daphnia magna following OECD Guideline 202. The test substance did not induce acute immobilisation of Daphnia magna at the nominal concentration limit test after 48 hours of exposure. The effects concentrations were based on the nominal test concentration of 100 mg Total Product (TP)/L. The 48-h EC₅₀ value was reported as >100 mg TP/L. No sublethal effects were noted during the definitive test; therefore, the 48‑h NOEC was reported as 100 mg TP/L.

 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L