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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

96-hour LC50 = >100 mg/L, NOEC = 100 mg/L, OECD 203 and EU Method C.1

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

A study was performed to assess the acute toxicity of the test material to rainbow trout (Oncorhynchus mykiss). The study was conducted in accordance with the standardised guidelines OECD 203 and EU Method C.1 under GLP conditions. The study was awarded a reliability score of 1 in accordance with the criteria set forth by Klimisch et al. (1997).

Following a preliminary range-finding test, seven fish were exposed to an aqueous solution of the test material, at a single concentration of 100 mg/L for a period of 96 hours at a temperature of 14 °C under semi-static test conditions. The test was conducted using juvenile rainbow trout. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.

Analysis of the test preparations at 0 and 72 hours (fresh media) and 24 and 96 hours (old media) showed measured test concentrations to be near nominal and so the results are based on nominal test concentrations only. It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L.

Under the conditions of this study, the 96-hour LC50 was greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L.