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EC number: 201-127-6 | CAS number: 78-62-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977 - 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- The study was conducted according to a Litton Bionetics Inc. method but full details are not available
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- no analytical purity stated; only single cultures tested, tester strains TA 102 or E. coli WP2 were not used in the study as required in the appropriate OECD test guideline
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Diethoxy(dimethyl)silane
- EC Number:
- 201-127-6
- EC Name:
- Diethoxy(dimethyl)silane
- Cas Number:
- 78-62-6
- Molecular formula:
- C6H16O2Si
- IUPAC Name:
- diethoxydimethylsilane
- Details on test material:
- - Name of test material (as cited in study report): Dimethyldiethoxysilane
- Physical state: colourless liquid
Constituent 1
Method
- Target gene:
- his operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- E. coli, other: W3110/polA+
- Species / strain / cell type:
- E. coli, other: P3478/polA-
- Species / strain / cell type:
- yeast, other: Saccharomyces cerevisiae (D4)
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S9-mix
- Test concentrations with justification for top dose:
- Main experiment:
- 0.001, 0.01, 0.1, 1, 5 µL/plate (with and without metabolic activation) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Solubility properties and relative non-toxicity to bacteria
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: -S9-mix: MNNG: 10 µg/plate (TA 1535, TA 100, D4), QM: 10 µg/plate (TA 1537), NF: 100 µg/plate (TA 1538, TA 98); +S9-mix: ANTH: 100 µg/plate (TA 1535, TA 100), AMQ: 100 µg/plate (TA 1537), AAF: 100 µg/plate (TA 1538, TA 98), DMNA: 100 µM/plate (D4)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Expression time (cells in growth medium): 48 h (Ames test), 24 h (DNA repair test)
NUMBER OF REPLICATIONS: single culture/test concentration
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth
- Evaluation criteria:
- A result is positive if the number of revertants is significantly increased compared with the solvent control to at least 2-fold of the solvent control for TA 100, TA 1535, TA 1537 and TA 1538, and 2-3 times the solvent control for TA 98 and D4.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium, other: TA 1535, TA1537, TA98, and TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- ≥1 µl/plate -S9-mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- yeast, other: Saccharomyces cerevisiae (D4)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- E. coli, other: W3110/po1A+
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- E. coli, other: P3478/po1A-
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- ADDITIONAL INFORMATION ON CYTOTOXICITY:
Marked cytotoxicity was observed in tester strain TA 1538 in the absence of metabolic activation at concentration ≥1 µl/plate. No relevant cytotoxicity was observed for the other tester strains at any concentration either in the presence or in the absence of metabolic activation.
Any other information on results incl. tables
Table 1: Experiment 1 Plate incorporation Number of revertants per plate
|
TA98 |
TA100 |
TA1535 |
||||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
36 |
44 |
No |
92 |
134 |
No |
28 |
14 |
No |
0.001 |
30 |
55 |
No |
76 |
117 |
No |
17 |
18 |
No |
0.01 |
26 |
52 |
No |
88 |
155 |
No |
16 |
21 |
No |
0.1 |
25 |
43 |
No |
81 |
142 |
No |
15 |
15 |
No |
1 |
34 |
53 |
No |
84 |
126 |
No |
16 |
13 |
No |
5 |
26 |
58 |
No |
75 |
125 |
No |
12 |
12 |
No |
Positive control |
>1000 |
>1000 |
No |
610 |
811 |
No |
>1000 |
299 |
No |
*solvent control with DMSO
Table 2: Experiment 1 Plate incorporation Number of revertants per plate
|
TA 1537 |
TA 1538 |
D4 |
||||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
14 |
15 |
No |
28 |
31 |
No |
44 |
154 |
No |
0.001 |
30 |
14 |
No |
15 |
31 |
No |
48 |
222 |
No |
0.01 |
19 |
26 |
No |
26 |
31 |
No |
42 |
64 |
No |
0.1 |
22 |
19 |
No |
23 |
29 |
No |
46 |
202 |
No |
1 |
14 |
18 |
No |
14 |
30 |
No |
43 |
234 |
No |
5 |
10 |
13 |
No |
15 |
35 |
No |
50 |
228 |
No |
Positive control |
>1000 |
392 |
No |
701 |
>1000 |
No |
746 |
242 |
No |
*solvent control with DMSO
Applicant's summary and conclusion
- Conclusions:
- No mutagenic effect was observed for the test substance tested up to cytotoxic concentration in any of the test strains in two independent experiments without and with metabolic activation. The test substance is non-mutagenic in test strains used.
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