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EC number: 619-220-9 | CAS number: 96499-68-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 - 24 Nov 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 21 July 1997
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dodecanoic acid, monoester with oxybis[propanediol]
- EC Number:
- 619-220-9
- Cas Number:
- 96499-68-2
- Molecular formula:
- not applicable, the substance is UVCB
- IUPAC Name:
- Dodecanoic acid, monoester with oxybis[propanediol]
Constituent 1
Method
- Target gene:
- his operon, trp operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor-supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of male rats, induced with phenobarbital and 5,6-benzoflavone
- Test concentrations with justification for top dose:
- Following concentrations were used in the main experiments:
First and second experiment (preincubation, all S. typhimurium strains): 2.44, 4.88, 9.77, 19.5, 39.1, 78.1 μL/plate without metabolic activation and 9.77, 19.5, 39.1, 78.1, 156, 313 μL/plate with metabolic activation
First and second experiment (preincubation, WP2 uvr A (pKM 101)): 9.77, 19.5, 19.5, 39.1, 78.1, 156, 313 μL/plate without metabolic activation and 39.1, 78.1, 156, 313, 625, 1250 μL/plate with metabolic activation - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: DMSO was selected because of the sufficient solubility of the test substance in DMSO.
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- sodium azide
- other: 2-nitrofluorene (2NF), 2-aminoanthracene (2AA)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation (first and second experiment)
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: triplicates in two independent experiments
DETERMINATION OF CYTOTOXICITY
- Method: inspection of bacterial background lawn and number of revertant colonies - Evaluation criteria:
- Acceptance criteria
The study was considered valid if:
- number of revertant colonies of the negative (solvent) and positive controls are in the historical control range
- no contamination
- mean number of revertant colonies in the positive control group is increased at least twice compared to the negative control group
Evaluation criteria
The number of revertant colonies in any strains at one or more doses is increased at least two times compared to the negative control group. There should be dose dependency or reproducibility as dose increases. - Statistics:
- Individual plates were counted for revertant colonies. The average and standard deviation of the number of revertant colonies were calculated. Statistical analysis was not performed.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- revertant number reduced to less than half compared to vehicle control group in exp. 1 and 2 starting at 39.1 µg/plate (-S9) and starting at 156 µg/ plate (+S9)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- revertant number reduced to less than half compared to vehicle control group in exp. 1 and 2 starting at 39.1 µg/plate (-S9) and starting at 313 µg/ plate (+S9)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- revertant number reduced to less than half compared to vehicle control group in exp. 1 and 2 starting at 39.1 µg/plate (-S9) and starting at 313 µg/ plate (+S9)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- revertant number reduced to less than half compared to vehicle control group in exp. 1 and 2 starting at 39.1 µg/plate (-S9) and starting at 156 µg/ plate (+S9)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- revertant number reduced to less than half compared to vehicle control group in exp. 1 and 2 starting at 313 µg/plate (-S9) and starting at 625 µg/ plate (+S9)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: A dose range finding study (preincubation) was conducted to determine the highest dose for the main study. The five tested concentrations were ranging from 4.88 until 5000 μg/plate. In the four S. typhimurium strains, growth inhibition was observed starting at 313 μg/plate with metabolic activation and starting at 78.1 μg/plate without metabolic activation. In E.coli WP2uvrA(pKM101), it was observed starting at 1250 μg/plate with metabolic activation and starting at 313 μg/plate without metabolic activation. Thus, the highest dose level of the main studies was selected at the lowest dose level at which the growth inhibition was observed in the range finding study: 313 μg/plate for the S. typhimurium strains with S9 mix and 78.1 μg/plate without S9 mix, 1250 μg/plate for E.coli WP2 uvr A (pKM 101) strains with S9 mix, 313 μg/plate without S9 mix. These were serially diluted by applying a geometric of 2 to produce 5 lower doses.
HISTORICAL CONTROL DATA
see Table 3 in "any other information on results incl. tables"
Any other information on results incl. tables
Table 1: Test results (experiment 1, preincubation)
With or without S9 Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate |
||||
Frameshift type |
Base-pair substitution type |
|||||
TA 1537 |
TA 98 |
TA 100 |
TA 1535 |
WP2 uvr A (pKM 101) |
||
- |
Solvent control (DMSO) |
10 ± 1 |
14 ± 1 |
76 ± 0 |
9 ± 1 |
82 ± 1 |
2.44 |
10 ± 2 |
14 ± 1 |
73 ± 2 |
9 ± 1 |
- |
|
4.88 |
9 ± 2 |
17 ± 1 |
73 ± 1 |
10 ± 1 |
- |
|
9.77 |
9 ± 1 |
15 ± 1 |
76 ± 3 |
11 ± 1 |
85 ± 2 |
|
19.5 |
11 ± 1 |
14 ± 1 |
75 ± 3 |
11 ± 1 |
76 ± 4 |
|
39.1 |
7 ± 2 * |
11 ± 1 * |
71 ± 3 * |
7 ± 1 * |
78 ± 3 |
|
78.1 |
0 ± 0 * |
5 ± 1 * |
62 ± 4 * |
7 ± 2 * |
77 ± 2 |
|
156 |
- |
- |
- |
- |
78 ± 2 |
|
313 |
- |
- |
- |
- |
79 ± 2 * |
|
Positive controls (µg/plate) |
9AA (80) |
2NF (5) |
SAZ (1.5) |
SAZ (1.5) |
4NQO (0.1) |
|
Mean (No. of colonies/plate) |
595 ± 5 |
719 ± 4 |
697 ± 7 |
514 ± 11 |
986 ± 5 |
|
+ |
Solvent control (DMSO) |
15 ± 1 |
32 ± 2 |
81 ± 3 |
8 ± 2 |
106 ± 3 |
9.77 |
17 ± 2 |
29 ± 1 |
87 ± 3 |
8 ± 1 |
- |
|
19.5 |
14 ± 1 |
30 ± 1 |
78 ± 2 |
10 ± 1 |
- |
|
39.1 |
15 ± 0 |
33 ± 2 |
77 ± 1 |
9 ± 2 |
103 ± 3 |
|
78.1 |
16 ± 2 |
29 ± 1 |
73 ± 3 |
9 ± 1 |
107 ± 3 |
|
156 |
10 ± 1 * |
31 ± 1 * |
72 ± 3 |
8 ± 0 |
108 ± 3 |
|
313 |
7 ± 3 * |
15 ± 4 * |
45 ± 4 * |
6 ± 2 * |
110 ± 2 |
|
625 |
- |
- |
- |
- |
96 ± 4 * |
|
1250 |
- |
- |
- |
- |
97 ± 4 * |
|
Positive controls (µg/plate) |
2AA(3) |
2AA(1) |
2AA(2) |
2AA(3) |
2AA(2) |
|
Mean (No. of colonies/plate) |
211 ± 8 |
393 ± 1 |
676 ± 26 |
152 ± 2 |
52121 |
* = reduced background lawn
2AA = 2-aminoanthracene
2NF = 2-nitrofluorene
4NQO = 4-nitroquinoline N-oxide
9AA = 9-aminoacridine
SAZ = sodium azide
Table 2: Test results (experiment 2, preincubation)
With or without S9 Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate |
||||
Frameshift type |
Base-pair substitution type |
|||||
TA 1537 |
TA 98 |
TA 100 |
TA 1535 |
WP2 uvr A (pKM 101) |
||
- |
Solvent control (DMSO) |
10 ± 1 |
15 ± 1 |
73 ± 1 |
10 ± 1 |
84 ± 3 |
2.44 |
9 ± 1 |
15 ± 1 |
80 ± 2 |
9 ± 1 |
- |
|
4.88 |
10 ± 2 |
15 ± 1 |
69 ± 2 |
10 ± 1 |
- |
|
9.77 |
10 ± 1 |
15 ± 1 |
70 ± 3 |
11 ± 1 |
90 ± 3 |
|
19.5 |
8 ± 1 |
16 ± 2 |
78 ± 2 |
9 ± 1 |
94 ± 5 |
|
39.1 |
7 ± 2 * |
12 ± 2 * |
70 ± 2 * |
7 ± 1 * |
92 ± 4 |
|
78.1 |
0 ± 0 * |
7 ± 1 * |
66 ± 3 * |
6 ± 1 * |
88 ± 3 |
|
156 |
- |
- |
- |
- |
82 ± 3 |
|
313 |
- |
- |
- |
- |
81 ± 1 * |
|
Positive controls (µg/plate) |
9AA (80) |
2NF (5) |
SAZ (1.5) |
SAZ (1.5) |
4NQO (0.1) |
|
Mean (No. of colonies/plate) |
621 ± 20 |
667 ± 20 |
661 ± 10 |
536 ± 11 |
883 ± 33 |
|
+ |
Solvent control (DMSO) |
17 ± 1 |
35 ± 2 |
72 ± 2 |
9 ± 1 |
133 ± 4 |
9.77 |
18 ± 1 |
34 ± 2 |
72 ± 1 |
10 ± 1 |
- |
|
19.5 |
18 ± 2 |
35 ± 2 |
77 ± 2 |
10 ± 1 |
- |
|
39.1 |
18 ± 2 |
32 ± 3 |
79 ± 3 |
11 ± 1 |
120 ± 4 |
|
78.1 |
17 ± 2 |
30 ± 2 |
76 ± 3 |
9 ± 1 |
127 ± 5 |
|
156 |
11 ± 1 * |
27 ± 2 * |
72 ± 4 |
8 ± 1 |
117 ± 3 |
|
313 |
7 ± 3 * |
17 ± 2 * |
61 ± 3 * |
6 ± 2 * |
117 ± 4 |
|
625 |
- |
- |
- |
- |
107 ± 3 * |
|
1250 |
- |
- |
- |
- |
101 ± 3 * |
|
Positive controls (µg/plate) |
2AA(3) |
2AA(1) |
2AA(2) |
2AA(3) |
2AA(2) |
|
Mean (No. of colonies/plate) |
189 ± 9 |
381 ± 3 |
597 ± 28 |
127 ± 6 |
532 ± 13 |
2AA = 2-aminoanthracene
2NF = 2-nitrofluorene
4NQO = 4-nitroquinoline N-oxide
9AA = 9-aminoacridine
SAZ = sodium azide
* = reduced background lawn
Table 3: Historical data (negative and positive controls)
Strain |
TA 98 |
TA100 |
TA 1535 |
TA 1537 |
WP2 uvr A (pKM 101) |
||||||
+/- S9 mix |
- |
+ |
- |
+ |
- |
+ |
- |
+ |
- |
+ |
|
Negative controls * |
Min |
9.6 |
14.3 |
60.5 |
64.9 |
4.7 |
3.9 |
3.3 |
8.4 |
73.9 |
87.3 |
Max |
26.1 |
37.6 |
110.9 |
125.1 |
15.5 |
14.9 |
11.7 |
21.4 |
167.6 |
198.2 |
|
Mean ± SD |
17.9 ± 3.0 |
25.9 ± 4.0 |
85.7 ± 10.2 |
95.0 ± 12.0 |
10.1 ± 2.1 |
9.4 ± 1.9 |
7.5 ± 1.4 |
14.9 ± 2.6 |
120.8 ± 17.3 |
142.7 ± 18.7 |
|
Positive controls |
Name |
2NF |
2AA |
SAZ |
2AA |
SAZ |
2AA |
9AA |
2AA |
4NQO |
2AA |
Min |
392.3 |
250.2 |
440.0 |
377.2 |
353.6 |
67.0 |
237.0 |
99.6 |
209.1 |
304.5 |
|
Max |
762.9 |
444.8 |
711.4 |
889.1 |
582.6 |
165.7 |
638.9 |
230.8 |
1163.3 |
610.5 |
|
Mean ± SD |
577.6 ± 87.4 |
347.5 ± 43.7 |
575.7 ± 55.0 |
633.1 ± 104.4 |
468.1 ± 47.4 |
116.3 ± 19.2 |
437.9 ± 128.5 |
165.2 ± 27.9 |
686.2 ± 160.7 |
457.5 ± 59.3 |
* = water for injection, DMSO, acetone, tetrahydrofuran, normal saline injection, sodium phosphate buffer
2AA = 2-aminoanthracene
2NF = 2-nitrofluorene
4NQO = 4-nitroquinoline N-oxide
9AA = 9-aminoacridine
SAZ = sodium azide
Applicant's summary and conclusion
- Conclusions:
- Based on the results of the conducted study the test substance did not exhibit mutagenic properties in bacterial cells.
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