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EC number: 254-942-4 | CAS number: 40498-13-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Dihydrochinizarin was not irritating in an in vivo skin irritation test
The test substance was not irritating in an eye irritation test
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1982-06-01 - 1982-06-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- not mentioned
- GLP compliance:
- no
- Remarks:
- Study conducted prior to implementation of OECD GLP
- Species:
- rabbit
- Strain:
- New Zealand White
- Remarks:
- HC:NZW
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: HACKING & CHURCHILL, Huntingdon, England
- Weight at study initiation: 3-4 kg
female
- Housing: individual cages
- Diet (e.g. ad libitum): ad libitum standard diet "Ssniff K 4", Versuchstierdiäten GmbH, Soest/Westfalen
- Water (e.g. ad libitum): ad libitum
- Acclimation period: not reported
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 - 25 °C
- Humidity (%): 40 - 60 %
- Photoperiod (hrs dark / hrs light): 12 /12
- Type of coverage:
- semiocclusive
- Preparation of test site:
- clipped
- Remarks:
- 6 x 6 cm2 clipped at both flanks with hair cutting machine
- Vehicle:
- water
- Remarks:
- the solid test substance was pasted with water
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 g of paste (mixture with water) dispensed on gauze patch (2.5 x2.5 cm2)
VEHICLE
- water
NEGATIVE CONTROL
6x6 cm2 clipped at other flank without treatment - Duration of treatment / exposure:
- 4h
- Observation period:
- 7 days
- Number of animals:
- 3
- Details on study design:
- TEST SITE
- Area of exposure: 2.5 x 2.5 cm2 at the flank
- Type of wrap if used: gauze fixed with elastic tape
REMOVAL OF TEST SUBSTANCE
- Washing (if done): with water
- Time after start of exposure: 4 hours
OBSERVATION TIME POINTS
1, 24, 48, 72 hours, 7 days
SCORING SYSTEM:
- Method of calculation: Draize (DRAIZE, J.H.,Appraisal of the safety of chemicals in Foods, Drugs and Cosmetics, Association of Food and Drug Officials the US, p.~6, 1959). - Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- erythema score
- Basis:
- animal: #2, #3
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 4
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- edema score
- Basis:
- animal: #1, #2, #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Other effects:
- - Other adverse systemic effects: not described, no mortalities
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Dihydrochinizarin was not irritating in the in vivo skin irritation test under the experimental conditions described in this report. Judgment is based on erythema and edema calculated as mean scores for each animal following grading at 24, 48 and 72 hours after removal of the test material, in consideration of reversibility. The following mean scores were calculated: erythema 0/0.33/0.33 and edema 0/0/0. Full reversibility of all scores was reached within 48 hours.
- Executive summary:
In a primary dermal irritation study, performed in accordance with OECD guideline 404, three adult New Zealand White rabbits were dermally patch (2.5 x 2.5 cm2) exposed for 4 hours to 0.5 g of test substance moistened with water. Test sites were covered with semi-occlusive dressing. The contralateral flank served as control. Animals were then observed for 7 days.
Irritation was scored by the method of (DRAIZE, J.H., Appraisal of the safety of chemicals in foods,drugs and cosmetics, Association of Food and Drug Officials of the US, p.46, 1959). 60 minutes after patch removal, all scores were 0.0. At the 24 hours scoring, 2/3 animals showed very slight erythema (score 1). No edema was observed. Full reversibility of all scores was reached within 48 hours.
In this study, the test substance is not a dermal irritant according to the criteria of LCLP, EU GHS (Regulation (EC) No 1272/2008).
Reference
Irritant/corrosive response data for each animal at each observation time up to removal of each animal from the test
Score at time point / Reversibility |
Erythema |
Edema |
Max. score: 4 |
Max. score: 4 |
|
60 min |
0/0/0 |
0/0/0 |
24 h |
0/1/1 |
0/0/0 |
48 h |
0/0/0 |
0/0/0 |
72 h |
0/0/0 |
0/0/0 |
Average 24h, 48h, 72h |
0/0.33/0.33 |
0/0/0 |
Reversibility*) |
c. |
c. |
Average time (unit) for reversion |
48 h |
|
*) Reversibility: c. = completely reversible; n.c. = not completely reversible; n. = not reversible
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01-06-1982 - 30-07-1982
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- no
- Remarks:
- Study conducted prior to implementation of OECD GLP
- Species:
- rabbit
- Strain:
- New Zealand White
- Remarks:
- HC:NZW
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: HACKING & CHURCHILL, Huntingdon, England
- Weight at study initiation: 3-4 kg
male
- Housing: individual cages
- Diet (e.g. ad libitum): ad libitum standard diet "Ssniff K 4", Versuchstierdiäten GmbH, Soest/Westfalen
- Water (e.g. ad libitum): ad libitum
- Acclimation period: not reported
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 - 25 °C
- Humidity (%): 40 - 60 %
- Photoperiod (hrs dark / hrs light): 12 /12 - Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1ml (max. 0.1g) of finely pulverised test material
VEHICLE
no - Duration of treatment / exposure:
- single application
- Observation period (in vivo):
- 7 days
- Number of animals or in vitro replicates:
- 3 animals
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
24 h following application (if necessary) with physiological saline
SCORING SYSTEM:
DRAIZE, J.H., The Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics, pp. 49-52. Association of Food and Drug Officials of the United States, Topeka, Kansas, 1965
Tool used to assess score: fluorescin - Irritation parameter:
- cornea opacity score
- Basis:
- animal: #1, #2, #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- iris score
- Basis:
- animal: #1, #2, #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 3
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: #2, #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Irritation parameter:
- chemosis score
- Basis:
- animal: #1, #2, #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Irritant / corrosive response data:
- Animals #1 and #3 showed slight conjunctival redness 24 hours following test substance application.
- Other effects:
- - Other observations: discarge was observed until 24 hours following application (animals not individually identified)
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test substance was not irritating in the eye irritation test under the experimental conditions described in this report.
- Executive summary:
In a primary eye irritation study, 0.1 ml (max. 0.1g) of the finely pulverised test substance (without vehicle) was instilled into the conjunctival sac of three adult New Zealand White rabbits. Animals then were observed for 7 days. Irritation was scored by the method of
DRAIZE, J.H. (The Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics, pp. 49-52. Association of Food and Drug Officials of the United States, Topeka, Kansas, 1965)
For two out of three animals, only slight to minimal conjunctival redness was observed until day 1. All effects were completely reversible within 48 h.
In this study, Dihydrochinizarin is not an eye irritant according to GHS.
Reference
Irritant/corrosive response data for each animal at each observation time up to removal of each animal from the test
Score at time point / Reversibility |
Cornea |
Iris |
Conjunctivae |
Chemosis |
Max. score: 4 |
Max. score: 2 |
Max. score: 3 |
Max. score: 4 |
|
60 min |
0/0/0 |
0/0/0 |
1/0/1 |
0/0/0 |
24 h |
0/0/0 |
0/0/0 |
1/0/0 |
0/0/0 |
48 h |
0/0/0 |
0/0/0 |
0/0/0 |
0/0/0 |
72 h |
0/0/0 |
0/0/0 |
0/0/0 |
0/0/0 |
Average 24h, 48h, 72h |
0/0/0 |
0/0/0 |
0.33/0/0 |
0/0/0 |
Area effected |
|
|
|
|
Reversibility*) |
|
|
c. |
|
Average time (unit) for reversion |
|
|
48 h |
|
*) Reversibility: c. = completely reversible; n.c. = not completely reversible; n. = not reversible
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Justification for classification or non-classification
Skin irritation
Based on available and relevant data, the test substance does not need to be classified for skin irritation according to regulation (EC) 1272/2008.
Eye irritation
Based on available and relevant data, the test substance does not need to be classified for skin irritation according to regulation (EC) 1272/2008.
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