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EC number: 824-772-0 | CAS number: 2060540-82-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- On December 5th, 2017 activated sludge was sampled at Valence’s Waste treatment Plant (France). The sludge was settled and the liquid supernatant discarded. The concentrated sludge was then suspended in the mineral solution. These two steps were repeated twice.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 484.9 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Test containers
The test was performed in white glass bottles of 610 mL.
Apparatus
Thermostatic chamber.
pHmeter
Common laboratory equipment
Measuring system (heads and oxitop controller)
The respirometric measurement is based on pressure measurement. If oxygen is consumed in a closed vessel at a constant temperature and the produced CO2 is trapped, a decrease of pressure happens and can be measured. If a gas is released, an overpressure develops. The measuring head stores these pressure variations for the whole duration of a test once started. These heads allow the measurement at a programmed time interval of the pressure in the bottle. The different parameters (volume of the bottle and volume of filling) are also parameterized allowing a direct measurement of the Biological Oxygen Demand in the bottles. The data are then recovered by infra-red transmission using the Oxitop® controller and transferred to computer.
Method
After rinsing the sludge, the concentration of suspended solids was then determined. An activated sludge volume was filtered on a 1.2 µm filter with a vacuum pump. Then, the filter was dried in drying oven at 105 °C and weighted. Final suspended solid concentration was 30 mg/L.
The ThOD of the test item was calculated from an elemental composition analysis. ThOD was 0.3204 mg O2 / mg test item.
Eight bottles were prepared according to table 1. The flasks were closed with the measuring heads and stirred with a magnetic stirrer.
The test was performed in an enclosure at 21 ° C ± 1 in the dark. The CO2 released was trapped by soda pellets and the oxygen consumption was derived from the measurement of pressure decrease. The BOD (Biological Oxygen Demand) values were extracted from the measurement heads using an "Oxitop® controller".
Nitrites and nitrates were measured at the beginning and the end of the test in order to apply corrections to biodegradation values with regards to the theoretical formation of these compounds when nitrogenous compounds are tested. - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- None.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 68.7
- Sampling time:
- 28 d
- Details on results:
- Based on a biodegradation rate higher than 60% in 14 days (experimental value: 80.0 and 77.9 % in 9 days) in the reference control, the test was carried out under good conditions. At the end of the test, measurements of Biological Oxygen Demand (BOD) in two replicates of the test item solution did not show a difference of more than 20% (experimental value:3.7%). The oxygen uptake of the control was not greater than 60 mg/l in 28 days (experimental value: 27.1 mg O2/L)
Based on a biodegradation rate higher than 25% in 14 days (experimental value: 70.1% in 13 days) in the toxicity control, the test item was not toxic to micro-organisms. No significant Biological Oxygen Demand was observed in the abiotic control over the test period.
With a biodegradation of 68.7% after 28 days, the test item is considered readily biodegradable. - Key result
- Parameter:
- ThOD
- Value:
- 0.305 g O2/g test mat.
- Results with reference substance:
- Based on a biodegradation rate higher than 60% in 14 days (experimental value: 80.0 and 77.9 % in 9 days) in the reference control, the test was carried out under good conditions.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Dodecylbenzene sulfonate stoechiometrically compounded with cyclohexylamine is readily biodegradable.
- Executive summary:
The objective of this study was to assess the aerobic ready biodegradability of dodecylbenzene sulfonate stoechiometrically compounded with cyclohexylamine by measurement of manometric respirometry over a 28-day period according to the OECD 301F guideline (July 1992). The test item, at 500 mg/L, as the sole source of carbon and energy, was incubated in a buffer-mineral salts medium which was inoculated with a mixed population of micro-organisms. The consumption of oxygen was determined from the change in pressure in the apparatus. Evolved carbon dioxide was absorbed in a solution of sodium hydroxide. The amount of oxygen taken up by the microbial population during biodegradation of the test substance (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of ThOD.
The test solutions were prepared by direct dilution of the test item.
The study is valid since:
Based on a biodegradation rate higher than 60% in 14 days (experimental value: 80.0 and 77.9 % in 9 days) in the reference control, the test was carried out under good conditions.
At the end of the test, measurements of Biological Oxygen Demand (BOD) in two replicates of the test item solution did not show a difference of more than 20% (experimental value:3.7%).
The oxygen uptake of the control was not greater than 60 mg/l in 28 days (experimental value: 27.1 mg O2/L)
Based on a biodegradation rate higher than 25% in 14 days (experimental value: 70.1% in 13 days) in the toxicity control, the test item was not toxic to micro-organisms.
Any significant Biological Oxygen Demand of the abiotic control over the test period was highlighted. The degradation of test item was not abiotic.
Since the test element is a multiconstituent (DDBS and CHA), the 10 -day window does not apply to the "readily biodegradable" character. 68.7% biodegradation were observed at the end of the test. The test item is considered readily biodegradable.
Reference
Biological Oxygen Demand(mg O2.L-1)
|
BOD mg O2/L |
|||||||
Time (Days) |
Control A |
Control B |
Reference control A |
Reference control B |
Test item solution A |
Test item solution B |
Toxicity control |
Abiotic control |
0.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.5 |
0 |
0 |
1.9 |
0 |
0 |
0 |
1.9 |
9.7 |
1.0 |
0 |
1.9 |
52.2 |
48.4 |
1.9 |
1.9 |
65.8 |
7.7 |
2.0 |
5.8 |
7.7 |
77.4 |
77.4 |
5.8 |
23.2 |
137 |
9.7 |
3.0 |
9.7 |
9.7 |
101 |
98.6 |
25.1 |
25.1 |
149 |
11.6 |
4.0 |
11.6 |
11.6 |
133 |
130 |
34.8 |
32.9 |
172 |
9.7 |
6.0 |
15.5 |
13.5 |
149 |
147 |
77.4 |
77.4 |
188 |
9.7 |
9.0 |
17.4 |
17.4 |
162 |
161 |
92.8 |
87 |
236 |
11.6 |
13.0 |
21.3 |
21.3 |
172 |
168 |
103 |
98.6 |
250 |
9.7 |
20.0 |
27.1 |
25.1 |
178 |
180 |
124 |
114 |
267 |
9.7 |
28.0 |
27.1 |
27.1 |
184 |
186 |
137 |
130 |
286 |
11.6 |
Nitrification correction |
24.4 |
24.3 |
181.7 |
183.5 |
133.4 |
127.5 |
284.5 |
0.0 |
Biodegradation percentage
Biodegradation (%) |
||||||
Time (Days) |
Reference control A |
Reference control B |
Test item solution A |
Test item solution B |
Toxicity control |
Abiotic control |
0.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.5 |
1.1 |
0.0 |
0.0 |
0.0 |
0.6 |
6.2 |
1.0 |
30.5 |
27.9 |
0.6 |
0.6 |
19.5 |
4.3 |
2.0 |
42.0 |
41.5 |
-0.6 |
10.7 |
39.2 |
1.9 |
3.0 |
54.3 |
52.3 |
10.0 |
10.0 |
41.9 |
1.2 |
4.0 |
72.2 |
69.6 |
15.0 |
13.8 |
48.2 |
-1.2 |
6.0 |
80.0 |
77.9 |
40.7 |
40.7 |
52.2 |
-3.1 |
9.0 |
86.0 |
84.4 |
48.8 |
45.1 |
65.8 |
-3.7 |
13.0 |
89.0 |
85.6 |
52.3 |
50.7 |
68.5 |
-5.6 |
20.0 |
90.3 |
91.6 |
64.7 |
56.9 |
73.1 |
-10.6 |
28.0 |
93.3 |
93.4 |
71.2 |
66.7 |
77.9 |
-10.0 |
28.0 with nitrification correction |
93.6 |
93.6 |
70.6 |
66.8 |
78.2 |
-15.7 |
Description of key information
The objective of this study was to assess the aerobic ready biodegradability of dodecylbenzene sulfonate stoechiometrically compounded with cyclohexylamine by measurement of manometric respirometry over a 28-day period according to the OECD 301F guideline (July 1992). The test item, as the sole source of carbon and energy, was incubated in a buffer-mineral salts medium which was inoculated with a mixed population of micro-organisms. The consumption of oxygen was determined from the change in pressure in the apparatus. Evolved carbon dioxide was absorbed in a solution of sodium hydroxide. The amount of oxygen taken up by the microbial population during biodegradation of the test substance (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of ThOD.
The test solutions were prepared by direct dilution of the test item.
The study is valid since:
Based on a biodegradation rate higher than 60% in 14 days (experimental value: 80.0 and 77.9 % in 9 days) in the reference control, the test was carried out under good conditions.
At the end of the test, measurements of Biological Oxygen Demand (BOD) in two replicates of the test item solution did not show a difference of more than 20% (experimental value:3.7%).
The oxygen uptake of the control was not greater than 60 mg/l in 28 days (experimental value: 27.1 mg O2/L)
Based on a biodegradation rate higher than 25% in 14 days (experimental value: 70.1% in 13 days) in the toxicity control, the test item was not toxic to micro-organisms.
Any significant Biological Oxygen Demand of the abiotic control over the test period was highlighted. The degradation of test item was not abiotic.
Since the test element is a multiconstituent, the 10 -day window does not apply to the "readily biodegradable" character. 72% biodegradation were observed at the end of the test. The test item is considered readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
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