Dec-9-en-1-ol

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 22, 2000 - March 20, 2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Qualifier:

according to guideline

Guideline:

other: Method for Testing the Biodegradability of Chemical Substances by Microorganisms

Version / remarks:

“Testing Methods for New Chemical Substances” (July 13, 1974, Kanpogyo No.5,
Planning and Coordination Bureau, Environment Agency, Yakuhatu No.615,
Pharmaceutical Affairs Bureau, Ministry of Health and Welfare, and 49 Kikyoku
No. 392, Basic Industries Bureau, Ministry of International Trade and Industry,
Japan)

GLP compliance:

not specified

Remarks:

Study carried out outside the EU.

Oxygen conditions:

aerobic

Inoculum or test system:

other: Mixture City sewage, river, lake and sea

Details on inoculum:

City sewage: Return sludge from sewage plants were collected.
River, lake and sea: Surface water and surface soil which are in contact with the atmosphere were collected.
Cultivation:
In about 30 minutes after ceasing the aeration of the sludge mixture, supernatant corresponding to about 1/3 of the whole volume was removed.
Then an equal volume of 0.1(w/v)% fresh synthetic sewage was added to the remaining portion and the mixture was aerated again. This procedure was repeated once every day. The cultivation was carried out at 25±2°C.
Cultivation temperature: 25 +/- 1°C
Cultivation duration: 28 days

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

(1) Control blank (n=1, Vessel #1)
In one test vessel, 10mL of the activated sludge was added to 290mL of the basal culture medium, so that the concentration of the activated sludge reached 30mg/L.

(2) Sludge + aniline (n=1, Vessel #10)
In one test vessel, 30mg of aniline and 10mL of the activated sludge was added to 290mL of the basal culture medium, so that the concentration of aniline and the activated sludge reached 100mg/L and 30mg/L, respectively.

(3) Sludge + test item (Test substance, n=2, Vessel #8 and 9)
In each test vessel, 30mg of the test item and 10mL of the activated sludge was added to 290mL of the basal culture medium, so that the concentration of the test item and the activated sludge reached 100mg/L and 30mg/L, respectively.

Reference substance:

aniline

Key result

Parameter:

other: Percentage biodegradation by BOD

Value:

60

Sampling time:

28 d

Key result

Parameter:

other: Percentage biodegradation by GC

Value:

100

Sampling time:

28 d

Details on results:

The average percentage biodegradation by BOD and GC were 60% and 100%, respectively. In addition, growth of the sludge was observed in the vessels of #8 and 9.

Key result

Parameter:

ThOD

Value:

>= 90.57 - <= 91.16 mg O2/g test mat.

Results with reference substance:

ThOD: 93.21

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

From the above result, it was concluded that test material was biodegraded by the activated sludge.

Description of key information

According to the test conditions following TG 301C, it was concluded that test material was biodegraded by the activated sludge.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable