Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetate

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from OECD SIDS Initial Assessment Report.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Principles of method if other than guideline:

Short term toxicity to Pseudokirchneriella subcapitata (algae) study was carried out for 72 hrs. The study was performed according to OECD Guideline 201 (Alga, Growth Inhibition Test).

GLP compliance:

not specified

Analytical monitoring:

yes

Remarks:

NPOC (non-purgeable organic carbon) analysis was performed.

Vehicle:

not specified

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM- Common name: Green algae

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Reference substance (positive control):

yes

Remarks:

Potassium dichromate was used as positive control in the study.

Duration:

72 h

Dose descriptor:

other: ErC50

Effect conc.:

0.83 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: no data

Duration:

72 h

Dose descriptor:

other: EbC50

Effect conc.:

0.44 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Other details not known

Validity criteria fulfilled:

not specified

Conclusions:

Based on the effect on growth rate and biomass of the test organism Pseudokirchneriella subcapitata, the 72hr ErC50and EbC50 values of test chemical was determined as 0.83 and 0.44 mg/L, respectively.

Executive summary:

Short term toxicity to Pseudokirchneriella subcapitata (algae) study was carried out for 72 hrs. The study was performed according to OECD Guideline 201 (Alga, Growth Inhibition Test). The study was based on the effects of the test compound on Pseudokirchneriella subcapitatain a static fresh water system. Pseudokirchneriella subcapitata was used as test organism. Analytical monitoring was done in which the NPOC (non-purgeable organic carbon) analysis was performed. Cultured algae were added to the WAF (Water – Accommodated Fraction) and Potassium dichromate was used as positive control in the study. Based on the effect on growth rate and biomass of the test organism Pseudokirchneriella subcapitata, the72hr-ErC50and EbC50 values of test chemical was determined as 0.83 and 0.44mg/L, respectively.Thus, based on EC50 value, it can be concluded that the substance can be considered as toxic to aquatic organisms and thus can be classifiedas aquatic acute category 1 / chronic 1 as per the CLP classification criteria.

Description of key information

1. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 0.34 and 0.026 mg/l, respectively and on the basis of AUG, the 72 hr EC50 and NOEC value was determined to be 0.093 and 0.026 mg/l, respectively.

2. Based on the effect on growth rate and biomass of the test organism Pseudokirchneriella subcapitata, the 72hr ErC50and EbC50 values of test chemical was determined as 0.83 and 0.44 mg/L, respectively.

Based on both studies it was concluded that the Reaction mass of 3H-​Indolium, 2-​[2-​[4-​(dimethylamino)​phenyl]​ethenyl]​-​1,​3,​3-​trimethyl-​ & acetate was toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

0.026 mg/L

Additional information

Data available for the structurally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test Reaction mass of 3H-​Indolium, 2-​[2-​[4-​(dimethylamino)​phenyl]​ethenyl]​-​1,​3,​3-​trimethyl-​ & acetate. The studies are as mentioned below:

 

In the first study from authoritative database short term toxicity to green algae study was carried out for 72 hrs. The study was based on the effects of the test compound on green algae in a static fresh water system. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 0.34 and 0.026 mg/l, respectively and on the basis of AUG, the 72 hr EC50 and NOEC value was determined to be 0.093 and 0.026 mg/l, respectively. Thus, based on the EC50 value, it can be concluded that the substance can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic acute category 1 / chronic 1 as per the CLP classification criteria.

 

 

Similarly second study was from secondary sources in which short term toxicity to Pseudokirchneriella subcapitata (algae) study was carried out for 72 hrs. The study was performed according to OECD Guideline 201 (Alga, Growth Inhibition Test). The study was based on the effects of the test compound on Pseudokirchneriella subcapitata in a static fresh water system. Pseudokirchneriella subcapitata was used as test organism. Analytical monitoring was done in which the NPOC (non-purgeable organic carbon) analysis was performed. Cultured algae were added to the WAF (Water – Accommodated Fraction) and Potassium dichromate was used as positive control in the study. Based on the effect on growth rate and biomass of the test organism Pseudokirchneriella subcapitata, the 72hr-ErC50and EbC50 values of test chemical was determined as 0.83 and 0.44mg/L, respectively. Thus, based on EC50 value, it can be concluded that the substance can be considered as toxic to aquatic organisms and thus can be classified as aquatic acute category 1 / chronic 1 as per the CLP classification criteria.

 

Based on both studies it was concluded that the Reaction mass of 3H-​Indolium, 2-​[2-​[4-​(dimethylamino)​phenyl]​ethenyl]​-​1,​3,​3-​trimethyl-​ & acetate was toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.