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EC number: 225-690-2 | CAS number: 5011-34-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 7 Sept. 2004 to 10 Sept. 2004
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Trimetazidine
- EC Number:
- 225-690-2
- EC Name:
- Trimetazidine
- Cas Number:
- 5011-34-7
- Molecular formula:
- C14H22N2O3
- IUPAC Name:
- 1-[(2,3,4-trimethoxyphenyl)methyl]piperazine
- Test material form:
- liquid
- Details on test material:
- No details are provided in the study report
Constituent 1
- Specific details on test material used for the study:
- No analytical data are provided on the test material in the study report. Batch N°538271/0124
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- To prepare the stock solution, the test item was dissolved or dispersed directly in LC reconstituted water. The obtained preparation was agitated mechanically for up to 24 hours in an attempt to completely dissolve or disperse the test item and homogenize the solution.
Water: reconstituted (LC). Prepared using deionized water with a conductivity less than 10 PS/cm. The final solution after autoclaving has a pH of 7.5 ± 0.3.
Test solutions was prepared by further dilution of the stock solution with water or directly from the test item. A solution containing a maximum of 100 mg/L of the test item plus two other range-finding concentrations was prepared. Test vessels was filled directly from the test solution containers immediately after preparation.
Test organisms
- Test organisms (species):
- Scenedesmus sp.
- Details on test organisms:
- Species: Scenedesmus subspicatus.
Strain No.: CCAP 276/20.
Reason for this choice: rapidly growing unicellular species commonly used in Europe for aquatic toxicity testing and recommended in OECD and EEC guidelines.
Cultured at: CIT.
Origin: Culture Collection of Algae and Protozoa, Institute of Freshwater Ecology, Far Sawrey, Ambleside, Cumbria, LA22 OLP, UK.
Culture method: the algae are cultured under sterile conditions and maintained at exponential growth rate by adding weekly aliquots of algal culture to fresh reconstituted water samples.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Measured but not specified in the report.
- Test temperature:
- between 24.0°C and 25.0°C.
- pH:
- In the control solution : pH between 7.39 to 8.40
In the test solution with adjusted pH : between 9.62 to 8.07
In the test solution without adjustement : between 8.88 to 7.90 - Dissolved oxygen:
- Not specified
- Salinity:
- Not specified
- Conductivity:
- less than 10 PS/cm
- Nominal and measured concentrations:
- Nominal concentrations : 0 ; 1 ; 10 & 100 mg/L
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 - < 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- With or without pH adjustment
- Results with reference substance (positive control):
- Not specified
Any other information on results incl. tables
Table 1 : pH & temperature
Nominal concentration (mg/L) |
pHat T0 hour |
pH at T72 hours |
0 |
7.39 |
8.40 |
100* |
9.62 |
8.07 |
100** |
8.88 |
7.90 |
* solution with no adjustment of pH
** solution with adjusted pH
Temperature measured in the dilution water between at T0 and T2 hours: 24.3 ° C
Temperature recorded in a dilution water beacker placed under the conditions of the study: minimum 24.0°C / maximum 25.0°C
Table 2: T72 hours - number of cells and calculation of the specific growth rate for each concentration
Nominal concentration (mg/L) |
Replicat |
Total number of cells at T72 hours (104/mL) |
Ln number of cells |
Average Ln number of cells |
Specific growth rate |
Growth rate inhibition |
0 |
1 2 3 |
85.0 89.5 106.0 |
13.65 13.70 13.87 |
13.74 |
0.06 |
|
1* |
1 2 3 |
118.0 161.0 152.0 |
13.98 14.29 14.23 |
14.17 |
0.07 |
-9.38 |
10* |
1 2 3 |
118.0 131.0 107.0 |
13.98 14.09 13.88 |
13.98 |
0.07 |
-5.28 |
100* |
1 2 3 |
2.2 1.7 3.9 |
10.00 9.74 10.57 |
10.10 |
0.01 |
80.29 |
100* |
1 2 3 |
7.0 9.1 7.6 |
11.16 1 1.42 11.24 |
11.27 |
0.03 |
54.54 |
Ln number of cells at T0 hour (10 000 cells) = 9,21
* solution with no adjustment of pH
** solution with adjusted pH
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 0-72h EC50b was estimated to be between 10 and 100 mg/L with or without pH adjustment.
- Executive summary:
A growth inhibition test on algae according to OECD 201 was performed.
The highest concentration no observed effect on algae growth (NOEC) at T72h was 10 mg/L, based on growth rate and biomass (p = 0.05).
At the concentration of 100 mg/L, inhibition of algae growth resulted from both a toxic effect of the test item (not pH-related) and its basic potential.
The 0-72h EC50r was estimated to be between 10 and 100 mg / L without pH adjustment and close to 100 mg/L with pH adjustment.
The 0-72h EC50b was estimated to be between 10 and 100 mg / L with or without pH adjustment.
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