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EC number: 220-744-1 | CAS number: 2885-00-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Remarks:
- comparable to guideline study GLP, Comparable to OECD 476 and OPPTS 870.5300 Reliability 1
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Deviations:
- yes
- Remarks:
- yes Information on number of cells used and other important parts of the experimental design are not provided.
- Principles of method if other than guideline:
- Similar to OECD 476 and OPPTS 870.5300
- GLP compliance:
- yes
- Type of assay:
- in vitro mammalian cell gene mutation tests using the thymidine kinase gene
Test material
- Reference substance name:
- Dodecane-1-thiol (CAS # 112-55-0)
- IUPAC Name:
- Dodecane-1-thiol (CAS # 112-55-0)
Constituent 1
Method
- Target gene:
- TK+
Species / strain
- Species / strain / cell type:
- other: L5178 TK+/- lymphoma cell
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- 30, 20.1, 13.5, 9.0, 6.0, 4.0, 2.7, and 1.8 µg/mL
- Vehicle / solvent:
- Solvent control used
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Remarks:
- ethylmethane sulfonate (620 ug/ml) without metabolic activation and 3-methylholanthrene (3 ug/ml) with metabolic activation
- Positive control substance:
- 3-methylcholanthrene
- ethylmethanesulphonate
Results and discussion
Test results
- Key result
- Species / strain:
- other: mouse lymphoma L5178Y cells mammalian cell line
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: other: Acceptable growth inhibition exhibited at 30 ug/ml.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- not measured/tested
Any other information on results incl. tables
Cells exposed to eight graded doses of test substance with and without metabolic activation did not exhibit a dose-dependednt increase in mutation frequency at the TK +/- locus. A test was considered positive if dose-related response at two or more test concentrations is at least two or three-fold higher than mutation frequency of solvent control.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: Negative
- Executive summary:
In a mammalian cell gene mutation assay focused on the T/K locus, L5178Y Mouse Lymphoma cells cultured in vitro were exposed to dodecane-1-thiol at concentrations at 1.8, 2.7, 4.0, 6.0, 9.0, 13.5, 20.1, and 30.0 µg/mL in the presence and absence of mammalian metabolic activation.
Concurrent positive controls were ethylmethane sulfonate (620 ug/ml) without metabolic activation and 3-methylcholanthrene (3 ug/ml) with metabolic activation. A negative and solvent control were also run concurrently. Under experimental conditions, dodecane-1thiol did not
exhibit a positive response and is considered to be not mutagenic in this test system. This study received a Klimisch score of 1 and is classified as reliable without restriction because the study adhered closely to OECD guidelines.
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