Nicotine dihydrogen ditartrate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

for read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical :
2.Study was conducted to investigate the effect of test chemical Nicotine sulfate on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs.

3.Effect of test material was observed on Oncorhynchus mykiss

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

2.Chemical analyzed with a Hewlett-Packard 5840A gas chromatograph equipped with model 7671 A auto injector and also with FID.

Vehicle:

not specified

Details on test solutions:

2. test material was extracted with the methylene chloride with the additions of saturated sodium borate buffer solution

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

2. Details on test organisms
Test organism Oncorhynchus mykiss
- Common name: Rainbow Trout
- Source: Test organisms obtain from the stock-culture units of the Environmental Research Laboratory-Duluth
- Weight at study initiation (mean and range, SD): 12.8 grams
- Feeding during test: Not fed 24 hrs before and during the test

Details on test organisms
Test organism Lepomis macrochirus
- Common name: Bluegill
- Source: Test organisms obtain from the stock-culture units of the Environmental Research Laboratory-Duluth
- Weight at study initiation (mean and range, SD): 0.5 g
- Feeding during test: Not fed 24 hrs before and during the test

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

2) 44.7 (40.8 to 47.6 mg/L CaCO3)

Test temperature:

2) 17.2 ° C (16.5 to 18.4 ° C)
3) 12 ° C

pH:

2) 7.39 (6.84 to 7.80)

Dissolved oxygen:

2) 8.7 (4.8 to 10.7 mg/L)

Details on test conditions:

2) Details on test conditions
TEST SYSTEM
- Test vessel: Glass aquaria
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top
- Renewal rate of test solution (frequency/flow rate): Flow-through
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2




TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Water obtain from Lake superior. 2 L proportional diluter with a dilution factor of 0.5 was used.
- Alkalinity: 43.0 mg/L CaCO3 (40.4 to 49.5 mg/L CaCO3)

OTHER TEST CONDITIONS
- Adjustment of pH: Yes
- Photoperiod: 12 hrs photoperiod was used
- Light intensity: 17 lumens at the surface of water.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality and all other parameters including pH, temperature were measured


- Results used to determine the conditions for the definitive study: Mortality

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

7.31 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % CI was 6.29- 8.50 mg/l

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Conclusions:

The test chemical is likely to be toxic to fish in the dose range of 4 - 7.31 mg/l

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical.The studies are as mentioned below:

Study was conducted to investigate the effect of test chemical on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs. According to the OECD guideline 202 test organisms were maintain in the appropriate system and follows all the criteria which meet the guideline.Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID.Both fishes obtain from the stock-culture units of the Environmental Research Laboratory-Duluth. Organisms not fed 24 hrs before and during the test.Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top.Test performed in duplicates with control which simultaneously run with the test chemical. Effect calculated by Trimmed Spearman- Karber method were used.As the test conducted on the two species of fish different effect concentrations were obtain at which 50 % mortality were observed. Based on the mortality of Oncorhynchus mykiss (Rainbow Trout) by the exposure of Nicotine sulfate for 96 hrs, the LC50 was determine to be 7.31 mg/l. And based on the mortality of Lepomis macrochirus (Bluegill), the LC50 was determine to be 4.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.

The above information was further supported by data for structurally similar read across, short term 96 h test was conducted to evaluate effect of test material on Oncorhynchus mykiss (Rainbow trout, age 13 -21 days fry) . The median lethal effect concentrtaion LC50 was observed to be 4 mg/l . Based on the effect concentration it can be concluded that test material is toxic to fish an d can be classified as aquatic chronic 2.

The test chemical  is  likely to be toxic to fish  in the dose range of 4 - 7.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.

Description of key information

Short-term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical.The studies are as mentioned below:

Study was conducted to investigate the effect of test chemical on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs. According to the OECD guideline 202 test organisms were maintain in the appropriate system and follows all the criteria which meet the guideline.Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID.Both fishes obtain from the stock-culture units of the Environmental Research Laboratory-Duluth. Organisms not fed 24 hrs before and during the test.Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top.Test performed in duplicates with control which simultaneously run with the test chemical. Effect calculated by Trimmed Spearman- Karber method were used.As the test conducted on the two species of fish different effect concentrations were obtain at which 50 % mortality were observed. Based on the mortality of Oncorhynchus mykiss (Rainbow Trout) by the exposure of Nicotine sulfate for 96 hrs, the LC50 was determine to be 7.31 mg/l. And based on the mortality of Lepomis macrochirus (Bluegill), the LC50 was determine to be 4.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.

The above information was further supported by data for structurally similar read across, short term 96 h test was conducted to evaluate effect of test material on Oncorhynchus mykiss (Rainbow trout, age 13 -21 days fry) . The median lethal effect concentrtaion LC50 was observed to be 4 mg/l . Based on the effect concentration it can be concluded that test material is toxic to fish an d can be classified as aquatic chronic 2.

The test chemical  is  likely to be toxic to fish  in the dose range of 4 - 7.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

7.31 mg/L

Additional information

Short-term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical.The studies are as mentioned below:

Study was conducted to investigate the effect of test chemical on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs. According to the OECD guideline 202 test organisms were maintain in the appropriate system and follows all the criteria which meet the guideline.Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID.Both fishes obtain from the stock-culture units of the Environmental Research Laboratory-Duluth. Organisms not fed 24 hrs before and during the test.Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top.Test performed in duplicates with control which simultaneously run with the test chemical. Effect calculated by Trimmed Spearman- Karber method were used.As the test conducted on the two species of fish different effect concentrations were obtain at which 50 % mortality were observed. Based on the mortality of Oncorhynchus mykiss (Rainbow Trout) by the exposure of Nicotine sulfate for 96 hrs, the LC50 was determine to be 7.31 mg/l. And based on the mortality of Lepomis macrochirus (Bluegill), the LC50 was determine to be 4.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.

The above information was further supported by data for structurally similar read across, short term 96 h test was conducted to evaluate effect of test material on Oncorhynchus mykiss (Rainbow trout, age 13 -21 days fry) . The median lethal effect concentrtaion LC50 was observed to be 4 mg/l . Based on the effect concentration it can be concluded that test material is toxic to fish an d can be classified as aquatic chronic 2.

The test chemical  is  likely to be toxic to fish  in the dose range of 4 - 7.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.