Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 228-794-6 | CAS number: 6359-10-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2010-03-15 to 2010-03-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992-07-17
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 2008-05-30
- Deviations:
- no
- GLP compliance:
- not specified
- Remarks:
- The study was conducted in accordance with ISO/IEC 17025.
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature, protected from light. - Analytical monitoring:
- no
- Remarks:
- No analyses of test concentrations were conducted. For the evaluation of the test the nominal concentration of the test substance in the test media was used.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: As a stock solution the nominal test concentrations of 100 mg/L was prepared. Test concentrations were prepared by dilution of this stock solution.
- Controls: Untreated test medium
- Evidence of undissolved material: No - Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM
- Common name: Guppy
- Length at study initiation: 1 - 3 cm
- Method of breeding: Population of parental and young fish was held in either 200 L or 40 L glass aquaria.
- Maintenance of the brood fish:
Illumination: 16 h per day
Temperature: 21 - 25 °C (thermostatic heating element)
Medium: Continuously aerated and filtered tap water. The bottom of the aquaria was covered with gravel (<1.5 cm).
Feed: Sera micropan, Sera vipan (Sera apanristic GmbH, D-52525 Heinsberg, Germany), daily. The fish were last fed 72 h before the start of the test series.
Validity: During 7 days before the test series, mortality in the batch of which fish were used for the test was less than 5 %.
ACCLIMATION
- Acclimation conditions: same as test
- Health during acclimation: During 7 days before the range finding test, no mortality in the batch of fish, which have been bred in the laboratory, was observed. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 36.6 - 37.5 °f H
- Test temperature:
- 21 - 24 °C,
- pH:
- 7.2 - 7.4
- Dissolved oxygen:
- Start:
Control: 8.0 mg/L O2
1.0 mg/L: 7.9 mg/L O2
10 mg/L: 8.2 mg/L O2
100 mg/L : 8.0 mg/L O2
End:
Control: 7.0 mg/L O2
1.0 mg/L: 6.9 mg/L O2
10 mg/L: 7.0 mg/L O2
100 mg/L: 6.6 mg/L O2 - Conductivity:
- 691 - 731 µS/cm
- Nominal and measured concentrations:
- Nominal: 0, 1.0, 10, and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria
- Type: open
- Material, fill volume: glass, 1 L
- Aeration: No
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Tap water
- Chlorine: 11.5 - 19.0 mg/L
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light / 8 h dark
EFFECT PARAMETERS MEASURED: Observations of the fish were made after 2, 24, 48, 72 and 96 h of exposure. Mortality of the fish was recorded at the same time intervals. Dead fish were removed immediately from the test medium and weight and length recorded.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 6.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: No
- Observations on body length and weight: No
- Other biological observations: No
- Mortality of control: 1 fish died in the blank control after 96 h of exposure.
- Other adverse effects control: No
- Abnormal responses: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Sublethal observations / clinical signs:
Table 1: Incidence of Mortality
Nominal
concentration
(mg/L)
Number of dead fish observed at different points in time after start of
exposure
2 h
24 h
48 h
72 h
96 h
Control
0
0
0
0
1
1.0
0
2
0
0
1
10.0
0
0
0
0
0
100.0
0
0
0
1
0
Table 2: Data of surviving fish after termination of the test
Nominal
concentration
(mg/L)
1.0
Length (mm)
19
25
17
24
-
-
-
Weight (mg)
39
81
35
116
-
-
-
10.0
Length (mm)
20
20
22
18
17
21
20
Weight (mg)
49
58
96
44
32
63
55
100.0
Length (mm)
18
22
20
19
18
19
-
Weight (mg)
47
86
48
51
42
45
-
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an 96 h acute toxicity test according to OECD Guideline 203 in Poecilia reticulata (Guppy), a LC50 of > 100 mg/L was determined.
- Executive summary:
In an acute toxicity test according to OECD Guideline 203, the acute lethal toxicity of an aqueous formulation containing 6.3 % test item to Poecilia reticulata (Guppy) was investigated under static exposure conditions over a period of 96 h. The nominal concentrations of Security Indicator BX Liquid were 1,10 and 100 mg/L, respectively. Therefore, this study represents a screening test. The individual test concentrations were prepared by dilution of a stock solution. No analytical verification of the test substance concentration was conducted. The evaluation of the test was based on the nominal concentrations of the test material, assuming the test compound to be stable in water over 96 h. After 96 h of exposure a total of 3 and 1 of seven fish were dead at 1 and 100 mg/L. No mortality was observed at 10 mg/L. 1 fish died in the blank control after 96 h of exposure. Toxic effects other than mortality, e.g. loss of coordination, hypo- or hyperactivity and swimming on the back, were not observed. The no-observed-effect concentration (NOEC, LC0) was >100 mg/L since the one fish that died is considered not to represent a significant effect as defined by the validity criteria of the test. Since minor effects were observed at 100 mg/L, the three dead fish observed at 1 mg/L were rated to be not a significant effect. 100 % mortality (LC100) could not be detected. Based on the binominal theory, the absence of mortality provides at least 99 % confidence that the LC50 is greater than the highest applied nominal test concentration of 100 mg/L (LC50 recalculated for active ingredient = 6.3 mg/L).
Reference
Description of key information
In an 96 h acute toxicity test according to OECD Guideline 203 in Poecilia reticulata (Guppy), using an aqueous formulation containing 6.3 % of the test item, an LC50 of > 100 mg/L was determined (LC50 recalculated for active ingredient = 6.3 mg/L).
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.