Mentha citrata, ext.

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of aquatic invertebrate for the test chemical :
1)To evaluate short term aquatic toxicity of test material on Daphnia.
2)To evaluate short term toxicity efficiency of test material on aquatic invertebrates.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material : Mentha citrata, ext.
- IUPAC name : Mentha citrata, ext.
- Molecular formula : C10H18O (Linalool) or C12H20O2 (Linalyl acetate)
- Molecular weight : 154.251- 196.288 g/mol
- Substance type: Organic
- Physical state: Clear liquid (Colorless to pale yellow)

Test organisms (species):

Daphnia magna

Details on test organisms:

1)- Common name: Water flea
- Strain: Straus 1820
-Age at study : ≥24 h old.

2)- Common name: water flea
-Source: CIBA-GEIGY's own testing facility culture
- Age at study initiation: 0-24 h old
- Food type: Scenedesmus subspicatus
supplemented by a suspension of Tetramin extract in such quantities that the feed is consumed within 24 h.

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

14, 48, 72 and 96 h.

Hardness:

1) 240 mg/L (CaCO3)

Test temperature:

1) 21-22°C.
2) 20 +/- 1 °C

pH:

1)7.7-8.0

Dissolved oxygen:

1) 94-100%;

Nominal and measured concentrations:

1)10, 18, 32, 58 and 100 mg/L

2)100, 58, 32, 18 and 10 mg/l.
Measured concentrations ranged between 85 and 99% of nominal
at time 0 and between 51 and 72% at time 48 h, the average
of both being between 70 and 81%.

Details on test conditions:

1) - Test vessel: beakers

-No. of daphnids: 5/replicates , 4 replicates/treatment
-Static without aeration in the dark; at 20±1°C in beakers, covered with glass watches and containing 100 mL of reconstituted water of hardness 240 mg/L(CaCO3)

2)- Test vessel: Glass vessle
- fill volume: filled with 2.5 L daphnid medium.
- 250 mg linalool was dissolved in and made up with daphnid medium to 2000 ml.
- No. of organisms per vessel: 20 daphnids per concentration
- 4 replicates of 5 daphnids each

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

15 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (95% CI: 13-17 mg/L)

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

59 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (53-65 mg/l, 95% CL)

Details on results:

EC50 values were calculated according to the maximum likelihood probit model

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical Mentha citrata, ext. is likely to be toxic to aquatic invertebrate in the dose range of 15 - 59 mg/l. Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of aquatic invertebrate of the test chemical Mentha citrata, ext. (85085 -49 -0).The studies are as mentioned below:

1.Short term toxicity test was performed according to OECD 202 for test chemical on Daphnia magna Straus 1820 was evaluated for 48 h

 >24 h old Daphnids were used as 5 per replicates and 4 replicates per treatment. The test was carried out in static without aeration in the dark; at 20±1°C in beakers covered with glass watches and containing 100 mL of reconstituted water of hardness 240 mg/L (CaCO3). The nominal concentrations subjected to the test were 10, 18, 32, 58 and 100 mg/L.

The effect concentration (EC50) of test chemical on Daphnia magna was observed to be 15 mg/l (95% CI: 13-17 mg/L).

Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.

2.

The test material was subjected to evaluate its efficiency for short term toxicity of aquatic invertebrates for 48 h according to OECD 202.

 Daphnia magna from CIBA-GEIGY's own testing facility culture were used for the test. Daphnids were inoculated in glass vessels containing approx. 2.5 l daphnid medium. Water was renewed 3 times weekly. At each renewal the daphnids were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of Tetramin extract in such quantities that the feed is consumed within 24 h.

24 h before test begin reproductive daphnia are separated from the young by sieving through a 0.8-mm sieve.

 

For each concentration and for the control 20 daphnids were used, in 4 replicates of 5 daphnids each. During the exposure the daphnids were not fed.Glass beakers were filled with 100 ml daphnid medium that had been aerated for 24 h before the test and covered with watch glasses during the test. The temperature was kept at20 +/- 1 °C during the test, there was no lighting in the test room and no aeration of the vessels during the test.Stock solution.250 mg test material was dissolved in and made up with daphnid medium to 2000 ml.

 Nominal test concentrations were 100, 58, 32, 18 and 10 mg/l. Controls were blanks (daphnid medium only). EC50 values were calculated according to the maximum likelihood probit model

 Immobilisation after 24 h was found in 17/20 daphnids and after 48 h in 19/20 daphnids. The 48-h EC50 was calculated to be 59 mg/l(53-65 mg/l, 95% CL).

Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.

Thus, based on the above summarised studies, Mentha citrata, ext and it’s structurally and functionallysimilar read across substance, it can be concluded that effect concetration value is in the range 15 - 49 mg/L. Thus, comparing this value with the criteria of CLP regulation, Mentha citrata, ext can be classified as " Acute aquatic 3" for short term toxicity for aquatic invertebrate .

Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate and can be categorize as "Not classified" as per CLP.

Description of key information

Short- term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of aquatic toxicity of the test chemical Mentha citrata, ext. (85085 -49 -0).The studies are as mentioned below:

1.Short term toxicity test was performed according to OECD 202 for test chemical on Daphnia magna Straus 1820 was evaluated for 48 h

 >24 h old Daphnids were used as 5 per replicates and 4 replicates per treatment. The test was carried out in static without aeration in the dark; at 20±1°C in beakers covered with glass watches and containing 100 mL of reconstituted water of hardness 240 mg/L (CaCO3). The nominal concentrations subjected to the test were 10, 18, 32, 58 and 100 mg/L.

The effect concentration (EC50) of test chemical on Daphnia magna was observed to be 15 mg/l (95% CI: 13-17 mg/L).

Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.

2.The test material was subjected to evaluate its efficiency for short term toxicity of aquatic invertebrates for 48 h according to OECD 202.

 Daphnia magna from CIBA-GEIGY's own testing facility culture were used for the test. Daphnids were inoculated in glass vessels containing approx. 2.5 l daphnid medium. Water was renewed 3 times weekly. At each renewal the daphnids were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of Tetramin extract in such quantities that the feed is consumed within 24 h.

24 h before test begin reproductive daphnia are separated from the young by sieving through a 0.8-mm sieve.

 

For each concentration and for the control 20 daphnids were used, in 4 replicates of 5 daphnids each. During the exposure the daphnids were not fed.Glass beakers were filled with 100 ml daphnid medium that had been aerated for 24 h before the test and covered with watch glasses during the test. The temperature was kept at20 +/- 1 °C during the test, there was no lighting in the test room and no aeration of the vessels during the test.Stock solution.250 mg test material was dissolved in and made up with daphnid medium to 2000 ml.

 Nominal test concentrations were 100, 58, 32, 18 and 10 mg/l. Controls were blanks (daphnid medium only). EC50 values were calculated according to the maximum likelihood probit model

 Immobilisation after 24 h was found in 17/20 daphnids and after 48 h in 19/20 daphnids. The 48-h EC50 was calculated to be 59 mg/l(53-65 mg/l, 95% CL).

Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.

Thus, based on the above summarised studies, Mentha citrata, ext and it’s structurally and functionallysimilar read across substance, it can be concluded that effect concetration value is in the range 15 - 49 mg/L. Thus, comparing this value with the criteria of CLP regulation, Mentha citrata, ext can be classified as " Acute aquatic 3" for short term toxicity for aquatic invertebrate .

Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate and can be categorize as "Not classified" as per CLP.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

49 mg/L

Additional information

Short- term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of aquatic toxicity of the test chemical Mentha citrata, ext. (85085 -49 -0).The studies are as mentioned below:

1.Short term toxicity test was performed according to OECD 202 for test chemical on Daphnia magna Straus 1820 was evaluated for 48 h

 >24 h old Daphnids were used as 5 per replicates and 4 replicates per treatment. The test was carried out in static without aeration in the dark; at 20±1°C in beakers covered with glass watches and containing 100 mL of reconstituted water of hardness 240 mg/L (CaCO3). The nominal concentrations subjected to the test were 10, 18, 32, 58 and 100 mg/L.

The effect concentration (EC50) of test chemical on Daphnia magna was observed to be 15 mg/l (95% CI: 13-17 mg/L).

Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.

2.The test material was subjected to evaluate its efficiency for short term toxicity of aquatic invertebrates for 48 h according to OECD 202.

 Daphnia magna from CIBA-GEIGY's own testing facility culture were used for the test. Daphnids were inoculated in glass vessels containing approx. 2.5 l daphnid medium. Water was renewed 3 times weekly. At each renewal the daphnids were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of Tetramin extract in such quantities that the feed is consumed within 24 h.

24 h before test begin reproductive daphnia are separated from the young by sieving through a 0.8-mm sieve.

 

For each concentration and for the control 20 daphnids were used, in 4 replicates of 5 daphnids each. During the exposure the daphnids were not fed.Glass beakers were filled with 100 ml daphnid medium that had been aerated for 24 h before the test and covered with watch glasses during the test. The temperature was kept at20 +/- 1 °C during the test, there was no lighting in the test room and no aeration of the vessels during the test.Stock solution.250 mg test material was dissolved in and made up with daphnid medium to 2000 ml.

 Nominal test concentrations were 100, 58, 32, 18 and 10 mg/l. Controls were blanks (daphnid medium only). EC50 values were calculated according to the maximum likelihood probit model

 Immobilisation after 24 h was found in 17/20 daphnids and after 48 h in 19/20 daphnids. The 48-h EC50 was calculated to be 59 mg/l(53-65 mg/l, 95% CL).

Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.

Thus, based on the above summarised studies, Mentha citrata, ext and it’s structurally and functionallysimilar read across substance, it can be concluded that effect concetration value is in the range 15 - 49 mg/L. Thus, comparing this value with the criteria of CLP regulation, Mentha citrata, ext can be classified as " Acute aquatic 3" for short term toxicity for aquatic invertebrate .

Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate and can be categorize as "Not classified" as per CLP.