Sodium 2-[[4-[3-(4-chlorophenyl)-4,5-dihydro-1H-pyrazol-1-yl]phenyl]sulphonyl]ethanesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

Limit test in August from 12 to 16, definitive test in September from 02 to 06, 2002

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no chemical analysis of the test substance concentrations was conducted

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

adopted 17 July, 1992

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Principles of method if other than guideline:

The study described in this report was conducted in accordance with ISO/IEC 17025

GLP compliance:

not specified

Analytical monitoring:

no

Details on test solutions:

The individual test concentrations were prepared by dilution of a 200 mg/l stock solution. However, the test material was not completely dissolved.

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Common name: guppy.
- Length at study initiation: 1-3 cm
- Start of the test: to start the test fish were transferred into the test vessels about 1 h after formation of the test media.

BREEDING SYSTEM
- Breeding: parentaland young fish population was held in two 250 l glass aquaria.
- Photoperiod: 14 hours a day.
- Medium: tap water from Schlieren
- Aeration: continuously aerated.
- Type of vessel: the bottom of the aquaria was covered with gravel (<1.5 cm).
- Renewal of test solution: very month, one third of the total volume of the aquaria was replaced by fresh tap water.
- Health check: during 7 days before the definitive test, mortality in the batch of which fish were used for the test was less than 5 %.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

Test vessels: 20 - 22.5 °C
Contorl: 20 - 22.5 °C

pH:

Test vessels: 7.9 - 8.6
Contorl: 8.0 - 8.6

Dissolved oxygen:

Test vessels: 6.5 - 7.0 mg/l
Contorl: 7.1 - 7.2 mg/l

Nominal and measured concentrations:

1, 10 and 100 mg/l nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: 1 l aquaria, all-glass.
- Aeration: medium aerated.
- No. of organisms per vessel: 7 fish per test concentration.
- No. of vessels per concentration: 1 replicate
- No. of vessels per control: 1 replicate

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: tap water from Schlieren. At least 1 h before the test the test, vessels were filled with the medium to allow equilibration of pH, oxygen and temperature.

OTHER TEST CONDITIONS
- Adjustment of pH: pH was not adjusted.
- Photoperiod: 16 hours photoperiod a day, supplied by overhead white fluorescent tubes.

EFFECT PARAMETERS MEASURED
Observations of the fish were made after 2, 24, 48, 72 and 96 h of exposure. Mortality of the fish was recorded at the same time intervals.
Dead fish were removed immediately from the test medium and weight and length recorded.
Fish were considered to be dead when they showed no reaction after touching the caudal fin or no visible breathing movements.

OBSERVETION AND MEASUREMENTS
- Oxygen: prior to the addition of fish to the test medium and after 2, 24, 48,72 and 96 h of exposure.
- pH: prior to the addition of fish to the test medium and after 2, 24, 48,72 and 96 h of exposure.
- Temperature: prior to the addition of fish to the test medium and after 2, 24, 48,72 and 96 h of exposure.

TEST CONCENTRATIONS
- Test concentrations: limit test with 100 mg/l.
- No of organisms per concentration 7 fish.
- Results: all 7 fish died after 24 h ot exposure. No mortality was observed in the control after 96 h of exposure.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10 - 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

During the test, all fish were dead at the nominal concentration of 100 mg/l after 48 h of exposure. No mortality was observed at 10 and 1 mg/l and in the control after 96 h of exposure.
Other toxic effects than mortality, e.g. loss of coordination, hypoactivity and swimming on the back, were not observed.
Based on these data the nominal lethal concentration (LC50) of test item was estimated to be 10-100 mg/l.
The no-observed-effect concentration (NOEC) was 10 mg/l. 100 % mortality (LC100) was observed at the nominal concentration of 100 mg/l after 48 h of exposure.

APPEARANCE OF TEST SOLUTION
The test material was not completely dissolved.

Sublethal observations / clinical signs:

Incidence of mortality observed.

Concentration (mg/l)	Number of dead fish observed at different points in time after start of exposure
	2 hrs	24 hrs	48 hrs	72 hrs	96 hrs
Control (0)	0	0	0	0	0
1	0	0	0	0	0
10	0	0	0	0	0
100	0	2	5	*	*
	Cumulative mortality observed at different points in time after start of exposure
Control (0)	0	0	0	0	0
1	0	0	0	0	0
10	0	0	0	0	0
100	0	2	7	*	*

Conclusions:

LC50 (96h): 10 - 100 mg/l (nominal)

Executive summary:

The acute lethal toxicity of test item to Poecilia reticulata (Guppy) was investigated under static exposure conditions over a period of 96 h. The applied nominal test concentrations were 1, 10 and 100 mg/l. Therefore, the study represents a screening test. The individual test concentrations were prepared by dilution of a 200 mg/l stock solution. However, the test material was not completely dissolved. No chemical analyses of the test concentrations were conducted.

During the test, all fish were dead at the nominal concentration of 100 mg/l after 48 h of exposure. No mortality was observed at 10 and 1 mg/l and in the control after 96 h of exposure.

Other toxic effects than mortality, e.g. loss of coordination, hypoactivity and swimming on the back, were not observed.

Based on these data the nominal lethal concentration (LC50) of test item was estimated to be 10-100 mg/l.

The no-observed-effect concentration (NOEC) was 10 mg/l. 100 % mortality (LC100) was observed at the nominal concentration of 100 mg/l after 48 h of exposure.

Conclusion

LC50 (96h): 10 - 100 mg/l (nominal)

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

July from 22 to 26, 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no chemical analysis of the test substance concentrations was conducted

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

no

Details on test solutions:

1000 mg/l in tap water as a suspension with solid particles. The test solution appeared as not homogeneous by visual assessment.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish.
- Source: Zoo-stumpe, Hildesheim.
- Length at study initiation: average 3.1 cm
- Weight at study initiation: average 0.36 g

ACCLIMATION
- Food: TetraMin Hauptfutter, Fa. Tetra.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

Test vesseld 21 - 22 °C
Control: 21 - 22 °C

pH:

Test vesseld: 7.87 - 8.07
Control: 7.93 - 8.06

Dissolved oxygen:

Test vesseld: 92 - 99 %
Control: 94 - 98 %

Nominal and measured concentrations:

10, 18, 32, 58 and 100 mg/l nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: 15 l volume, aquaria.
- Type (delete if not applicable): open / closed
- No. of organisms per vessel: 10 fish
- No. of vessels per concentration: 1 replicate.
- No. of vessels per control: 1 replicate.

OTHER TEST CONDITIONS
- Photoperiod: diffused room light, 12 - 16 hours daily rhythm.

TEST CONCENTRATIONS
- Test concentrations: 10 and 100 mg/l

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

41 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Lower test concentration causing 100 % of mortality: 58 mg/l
Highest test concentration causing no mortality: 18 mg/l

Concentration-dependent slight haze and bluish opalescent shimmer were observed in all assays.
In the test concentrations 32 - 100 mg/l, a slight to strong foaming occurred.
After 24 h, a slight concentration-dependent substance precipitate at the edge of the aquaria was found from the test concentration 32 mg/l.

Sublethal observations / clinical signs:

Cumulative mortality

Concentration (mg/l)	Cumulative mortality (%) at
	24 hrs	48 hrs	72 hrs	96 hrs
100	100	100	100	100
58	40	70	100	100
32	0	0	0	10
18	0	0	0	0
10	0	0	0	0
Control	0	0	0	0

Values for each observation time with confidence level 95 (%)

Test duration (h)	LC50 (mg/l)	CI 95 %
24	59	51 - 69
48	49	38 - 62
72	44	32 - 58
96	41	37 - 46

Validity criteria fulfilled:

yes

Remarks:

mortality in the control equal/lower than 10 % and concentration of dissolved oxygen equal/higher than 60 %

Conclusions:

LC50 (96h): 41 mg/l (nominal)

Executive summary:

The acute lethal toxicity of the test substance to Danio rerio was investigated under static exposure conditions over a period of 96 hours. 10 fish were exposed to each one of the follwoing nominal dose levels: 10, 18, 32, 58 and 100 mg/l. No chemical analysis of the test substance concentrations was conducted.

Concentration-dependent slight haze and bluish opalescent shimmer were observed in all assays. In the test concentrations 32 - 100 mg/l, a slight to strong foaming occurred. After 24 h, a slight concentration-dependent substance precipitate at the edge of the aquaria was found from the test concentration 32 mg/l.

The lower test concentration causing 100 % of mortality was 58 mg/l, while the highest test concentration causing no mortality was 18 mg/l

Conclusion

LC50 (96h): 41 mg/l (nominal)

Description of key information

LC50 (96h): 41 mg/l (nominal)

Additional information

The acute lethal toxicity of Fluorescent Brightener 312 to Danio rerio and Poecilia reticulata was investigated in two different experiments, both conducted under static exposure conditions and over a period of 96 h.

10 Danio rerio fish were exposed to each one of the follwoing nominal dose levels: 10, 18, 32, 58 and 100 mg/l. The lower test concentration causing 100 % of mortality was 58 mg/l, while the highest test concentration causing no mortality was 18 mg/l. The LC50 was estimated to be 41 mg/l.

The applied nominal test concentrations in the case of Poecilia reticulata were 1, 10 and 100 mg/l. During the test, all fish were dead at the nominal concentration of 100 mg/l after 48 h of exposure. No mortality was observed at 10 and 1 mg/l and in the control after 96 h of exposure. Other toxic effects than mortality, e.g. loss of coordination, hypoactivity and swimming on the back, were not observed. Based on these data the nominal lethal concentration (LC50) of test item was estimated to be 10-100 mg/l.

In both the cases, no chemical analysis of the test substance concentrations was conducted. In the first mentioned study, concentration-dependent slight haze and bluish opalescent shimmer were observed in all assays; in the test concentrations 32 - 100 mg/l, a slight to strong foaming occurred and after 24 hours a slight concentration-dependent substance precipitate at the edge of the aquaria was found from the test concentration 32 mg/l.

The test material resulted to be not completely dissolved also in the case of the other experiment.