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Diss Factsheets
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EC number: 211-669-5 | CAS number: 683-10-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 3 hours
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- OECD 209 guideline followed to GLP, June 2016
Assessed to be scientifically valid - Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Specific details on test material used for the study:
- COCAMIDOPROPYL PG - DIMONIUM CHLORIDE PHOSPHATE; LOT # 50113A16
Date and Quantity Received: 07 Mar 16
Physical Description: Clear liquid
Storage Conditions: Room temperature
Purity: 41% actives in water.
The substance that has been used in this test is not an alkyl betaine but can hydrolyse to betaines under biological conditions through hydrolysis of the phosphate ester. In the absence of primary data for alkyl betaines and cetyl betaine in particular, it has ben assessed as a reasonable surrogate to assess this end point until primary data can be accessed. - Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- Substance diluted directly into water.
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Samples of activated sludge were obtained from the Brazos Valley Sewer (Sugar Land, Texas) and aerated during transport and storage
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- pH:
- Buffered to pH 7.0
- Nominal and measured concentrations:
- Nominal 62.5, 125, 250, 500, 1000 mg/l actives
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Duration:
- 3 h
- Dose descriptor:
- EC100
- Effect conc.:
- ca. 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Duration:
- 3 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Details on results:
- At concentrations up to and including 500 mg/l, there was an enhancement of oxygen consumption
The test report cites a calculated EC50 of 495 mg/l that is clearly incorrect in view of enhanced effects seen at 500 mg/l. - Results with reference substance (positive control):
- Considered valid.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance enhanced microbial activity at up to 500 mg/l actives in the main test (although in a range finder, there was up to 25% inhibition noted at 100 mg/l). At 1000 mg/l in the range finder and main studies, there was a clear inhibition, with over 90% inhibition in some replicates. The EC50 is not possible to calculate, but is in the range 500 - 1000 mg/l
- Executive summary:
The results suggest that the substance should be readily biodegrable at concentrations below 500 mg/l actives in view of enhanced activity; however, at higher concentrations, there appears to be significant inhibition.The EC50 is not possible to calculate, but is in the range 500 - 1000 mg/l.
The substance that has been used in this test is not an alkyl betaine but can hydrolyse to betaines under biological conditions through hydrolysis of the phosphate ester. In the absence of primary data for alkyl betaines and cetyl betaine in particular, it has ben assessed as a reasonable surrogate to assess this end point until primary data can be accessed.
Reference
Description of key information
OECD 209 Sludge inhibition
The results suggest that the substance should be readily biodegrable at concentrations below 500 mg/l actives in view of enhanced activity; however, at higher concentrations, there appears to be significant inhibition.
The EC50 is not possible to calculate, but is in the range 500 - 1000 mg/l
Key value for chemical safety assessment
- EC50 for microorganisms:
- 500 mg/L
- EC10 or NOEC for microorganisms:
- 500 mg/L
Additional information
The substance that has been used in this test is not an alkyl betaine but can hydrolyse to betaines under biological conditions through hydrolysis of the phosphate ester. In the absence of primary data for alkyl betaines and lauryl betaine in particular, it has been assessed as a reasonable surrogate to assess this end point until primary data can be accessed.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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