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Diss Factsheets
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EC number: 203-726-8 | CAS number: 109-99-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-07-21 to 2014-07-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 455: “Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists”, adopted 02. Oct. 20121
- Deviations:
- yes
- Remarks:
- The stock solution of the test item in DMSO: The concentration of the stock solution was 0.045 mol/L instead of 0.01 mol/L. This can be seen as uncritical because the higher concentrated solutions of the test item showed no endocrine activity.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- certified by Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Kaiser-Friedrich-Str. 7, D-55116 Mainz, Germany
- Type of method:
- in vitro
- Remarks:
- genetically modified Saccharomyces cerevisiae used
Test material
- Reference substance name:
- Tetrahydrofuran
- EC Number:
- 203-726-8
- EC Name:
- Tetrahydrofuran
- Cas Number:
- 109-99-9
- Molecular formula:
- C4H8O
- IUPAC Name:
- tetrahydrofuran
- Test material form:
- liquid
1
Test animals
- Species:
- other: Yeast (Saccharomyces cerevisae)
- Strain:
- other: Yeast, genetically modified with human genes
- Remarks:
- hERa and hAR receptor, Expression plasmid carrying response elements and reporter gene lacZ
- Details on test animals or test system and environmental conditions:
- Origin
The YES-YAS Assay Kit including Saccharomyces cerevisiae was obtained from Xenometrix AG, Gewerbestrasse 25, CH-4123 Allschwil, Switzerland.
Batch no.: N05-2785, expiry date: Mar. 2015
Culture
Three days before the start of the experiment, the yeast cultures (YES and YAS) were prepared and incubated at 32 ± 1 °C for 72 hours, until the cultures were clearly turbid. After incubation, the optical density was measured with a microplate reader. The cultures were diluted appropriately and then used for the experiment.
Administration / exposure
- Duration of treatment / exposure:
- 72h at 32°
- Frequency of treatment:
- once
- Duration of test:
- 3 days before test, ca. 3 days during test
Doses / concentrations
- Remarks:
- 8 different concentrations: 1.43*10-7, 4.52*10-7, 1.43*10-6, 4.52*10-6, 1.43*10-5, 4.52*10-5, 1.43*10-4, 4.52*10-4 mol/L
- Control animals:
- yes
- Details on study design:
- The yeast cultures were prapared 3 days before the experiment. On the day of the assay, the cultures were checked for growth with a microplate reader. Then the plates were prepared according to the XenoScreen kit: There are YES Agonist, YAS Agonist, Yes Antagonist and YAS Antagonist plates. For each sort of plates 8 different concentrations of the test substance or positive controls were added in duplicate. After ca. 50h the absorption values at 570nm (for detection of the ß-galactosidase activity and therefore agonistic or antagonistic activity of the test substance) and at 690nm (for cell density and therefore cell growth) were taken by a microplate reader. Then growth factor, ß-galactosidase activity and induction rate are calculated and dose response curves were drawn. A dilution of a test substance is considered to have an agonistic effect if the induction rate is =10 and a possible antagonistic effect if the induction rate is = 50, respectively.
- Statistics:
- not made
Results and discussion
Effect levels
- Key result
- Basis for effect level:
- other:
- Remarks on result:
- not determinable due to absence of adverse toxic effects
- Remarks:
- No endocrine activity observed in this test system.
Any other information on results incl. tables
After incubation with test item and yeast cells, no cytotoxicity was observed.
The test item Tetrahydrofuran showed no positive result in any assay: The values for galactosidase activity and induction ratio of all test item dilutions lay in the same range as the values of the respective control. No dose-response correlation was visible.
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this test system, the test item Tetrahydrofuran is considered to have no estrogenic and androgenic agonistic and antagonistic activity in the YES YAS Assay.
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