Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-367-7 | CAS number: 106-15-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- of read across substance
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two short term toxicity study of fish for the test chemical :
2.This study was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio).
3.Bioassay was conducted to evaluate toxicity of test material on fish. - GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- 2. The test substance is insoluble in water as well as in the solvents namely, ethanol, acetone, acetonitrile, N,N-Dimethyl formamide, Tertiary butyl alcohol, chloroform. Therefore, the test substance was used as it is and test solution was prepared by dissolving 50 mg, 100 mg, & 150 mg of the test substance in 10 liters deionized water with continuous stirring for achieving test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, respectively.
- Test organisms (species):
- other: 2. Danio rerio , 3.Oryzias latipes
- Details on test organisms:
- .TEST ORGANISM
- Common name: Zebra Fish
- Strain: Danio rerio
- Source: Ashish Fish Center, Dharampeth, Nagpur, MS (India)
- Age at study initiation (mean and range, SD): 3-4 months
- Length at study initiation (length definition, mean, range and SD): Average 3.63 cm
- Weight at study initiation (mean and range, SD): 0.399 g (approx.)
- Method of breeding: No data
- Feeding during test: Food was withdrawn 1 day before and during exposure
- Food type: Standard brand feed
- Frequency: Once daily
ACCLIMATION
- Acclimation period: seven days
- Acclimation conditions (same as test or not): same
- Type and amount of food: Standard brand feed
- Feeding frequency: Once in a day up to sixth day of acclimatization
- Health during acclimation (any mortality observed): During last 48 hours mortality was < 5 %
2.The fish were held in the laboratory at 20-23oC for 2-4 weeks prior to use in bioassay and were fed an excess of dry commercial diet pellet for trout daily. The Ssh were acclimated to the test system for 2 days prior to testing without feeding. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 2)25 milligrams per liter Calcium ca
- Test temperature:
- 21- 36°C
- pH:
- 6-8
- Dissolved oxygen:
- 1) 7.4 mg/l
2)≥5-≥8.5 milligrams per liter - Nominal and measured concentrations:
- 1) 5 mg/L, 10 mg/L & 15 mg/L
- Details on test conditions:
- 1) TEST SYSTEM
- Test vessel: The test vessels used were all glass bowl aquaria of 12 inch each.
- Type: open
- Material, size, headspace, fill volume:
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized water was used
OTHER TEST CONDITIONS
Photoperiod: 12 hours light and 12-hour darkness
2)Ten fish were tested at each concentration which l'ncreases by geometrical progression of 1.4 in 3-5 / glass beaker held in constant temperature (21-22oC) water bath without feeding or artihcial aeration. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96 hr LC50 value was evaluated to be >15 mg/l.
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of fish of the test chemical .The studiesare as mentioned below:
For structurally similar read across substance , a 96 h study was conducted for test material on Danio rerio, at various test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, the fishes were found to be freely swimming in the bowl aquaria and no mortality were observed at test concentrations. No mortalities were found in the control aquaria. Based on nominal concentrations, experimental median lethal concentration [LC-50 (96 h)] for test material on Danio rerio was found to be >15 mg/L.
For , another structurally similar read across substance , the lethal concentration (LC50) value of test material in fish (Oryzias latipes) in a 48 hr study on mortality effect was found to be 2.4 mg/L.
Thus, during the exposure period of 96 hr, test chemical was considered as non-toxic at environmental relevamt concentrations.
Reference
Description of key information
Short term toxicity to fish:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of fish of the test chemical .The studiesare as mentioned below:
For structurally similar read across substance , a 96 h study was conducted for test material on Danio rerio, at various test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, the fishes were found to be freely swimming in the bowl aquaria and no mortality were observed at test concentrations. No mortalities were found in the control aquaria. Based on nominal concentrations, experimental median lethal concentration [LC-50 (96 h)] for test material on Danio rerio was found to be >15 mg/L.
For , another structurally similar read across substance , the lethal concentration (LC50) value of test material in fish (Oryzias latipes) in a 48 hr study on mortality effect was found to be 2.4 mg/L.
Thus, during the exposure period of 96 hr, test chemical was considered as non-toxic at environmental relevamt concentrations.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 15 mg/L
Additional information
Short term toxicity to fish:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of fish of the test chemical .The studiesare as mentioned below:
For structurally similar read across substance , a 96 h study was conducted for test material on Danio rerio, at various test concentrations of 5 mg/L, 10 mg/L & 15 mg/L, the fishes were found to be freely swimming in the bowl aquaria and no mortality were observed at test concentrations. No mortalities were found in the control aquaria. Based on nominal concentrations, experimental median lethal concentration [LC-50 (96 h)] for test material on Danio rerio was found to be >15 mg/L.
For , another structurally similar read across substance , the lethal concentration (LC50) value of test material in fish (Oryzias latipes) in a 48 hr study on mortality effect was found to be 2.4 mg/L.
Thus, during the exposure period of 96 hr, test chemical was considered as non-toxic at environmental relevamt concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)