Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 605-399-0 | CAS number: 165252-70-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19/09/2011 - 19/03/2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP, Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 2010
- Deviations:
- yes
- Remarks:
- see Table A7.4.1.4-5
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- At 30 min and 3 hours.
- Vehicle:
- no
- Details on test solutions:
- Not applicable
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Nature: activated sludge
Species: not applicable
Strain: not applicable
Source: Municipal wastewater treatment plant, collected from the aeration tank
Sampling site: Pforzheim, Germany
Laboratory culture: No
Method of cultivation
Preparation of inoculum for exposure: The sludge was used one day after collection. It was settled for about 10 minutes and the upper layer with finer solids was decanted. Before starting the test, it was washed three times with chlorine free tap water by centrifugation (10 minutes at 3000 rpm). After centrifuging, the supernatant was decanted and discarded and the sludge was re-suspended in chlorine free tap water. This procedure was repeated twice.
The mixed liquor suspended solids (MLSS) were adjusted to a concentration of 3.0 g/L (± 10 %). The activated sludge was continuously aerated at the test temperature, the solids did not settle down.
Pretreatment: None
Initial cell concentration: Not reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none
- Hardness:
- Not reported
- Test temperature:
- 17.6 – 20.0 °C (measured during O2-determination at 30 min and 3 h after start of respiration)
- pH:
- 7.78 – 8.22
- Dissolved oxygen:
- Not reported
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Not reported
- Details on test conditions:
- Culturing apparatus: BOD flasks
Number of culture flasks/concentration:
- 3 replicates for the negative, the abiotic and the heterotrophic control (ATU)
- 3 replicates each at 1000mg/L for total oxygen uptake and heterotrophic oxygen uptake (ATU)
- 1 replicate each for the positive control (DCP), the 10 and 100 mg/L total oxygen uptake and heterotrophic uptake samples
Aeration device: air pump
Measuring equipment: O2-electrode: Dissolved Oxygen Hand-Held Meter OXI 340, WTW
Test performed in closed vessels due to significant volatility of TS: No - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (DCP)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Total inhibition, inhibition of the heterotrophic organisms and inhibition of nitrifying bacteria
- Details on results:
- NOEC = 1000 mg/L; EC50 > 1000 mg/L
- Results with reference substance (positive control):
- For the total oxygen uptake, the EC50 was determined to be 11.29 mg/L after 30 min and 6.17 mg/L after 3 h. These values were within the recommended range of 2 to 25mg/L. For the heterotrophic oxygen uptake, the EC50 was determined to be 23.03 after 30 min and 15.60 mg/L after 3h. These values were within the recommended range of 5 to 40 mg/L. For the oxygen uptake due to nitrification the EC50 was determined to be 2 mg/L after 30 min and 1.67 mg/L after 3h. Both values were within the recommended range of 0.1 to 10 mg/L
- Reported statistics and error estimates:
- The statistical evaluation was performed for specific respiration rate of the control and highest concentration using SAS® (2002–2008). The calculation was performed using SAS Software service pack 9.2. The NOEC was determined using the t-Test pooled. P-values below 0.05 showed statistically significant differences to the control. A test for normality of the data was performed by calculating the Shapiro-Wilk’s statistic. The EC50-values for the reference test item DCP were evaluated using probit analysis following normal and logistic distribution. In the case of the nitrifiers the EC50 value for DCP after 3 h was extrapolated with a linear regression curve. The EC50 calculations were based on the percentage of inhibition.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item dinotefuran technical had no toxic effects on the oxygen uptake at the highest concentration tested (1000 mg/L). Hence, the EC50 of dinotefuran technical is > 1000 mg/L.
Reference
Table 1: Deviations from the study plan
Section of study plan |
Deviation |
Reason |
Impact on the study |
2.1 Test item |
The date of the certificate of analysis changed from 05 September 2011 to 24 November 2010. |
Mistake during study plan preparation. |
None. |
2.2 Reference item |
The stock solution of the reference item was not prepared with 0.5 g with a final volume of 500 mL but with 0.25 g filled up to a final volume of 250 mL. |
Mistake during study plan preparation |
None, since the concentration was the same |
2.5 Microbial Inoculum |
The sludge was not used on the same day as collec-tion but on the next day. |
Mistake in study performance |
None, since the guideline allows storage up to two days |
2.5 Microbial Inoculum |
The sludge was not centrifuged for 20 min at 4500 rpm but for 10 min at 3000 rpm. |
Mistake in study performance. |
None, since the sludge was centrifuged to produce a clear supernatant and pellet of sewage solids, therefore fulfilling guideline criteria as the guide-line does not stipulate a speed of centrifugation. |
2.8 Test conditions |
The temperature of the test decreased to 17.6 °C. |
Mistake in study performance |
None, since the deviation did not have an adverse effect on the validity and outcome of the study. The guideline recommends a temperature range of 20 ± 2 °C. The lowest recorded temperature was 17.6°C. However, the test item showed no adverse effect and the EC50 value of the reference item was within the recommended range. Therefore, the temperature difference of 0.4°C is considered not to have had an adverse effect on the validity and the outcome of the study. |
2.7.2 Procedure of the Test |
The pH measured ranged from 7.92 – 8.22. This is above the recommended range of 7.5 ± 0.5. |
Mistake in study performance |
None, since the deviation did not have an adverse effect on the validity and outcome of the study. The test item showed no adverse effect and the EC50 of the reference item was within the recommended range. Therefore, the pH difference of 0.2 is considered not to have had an adverse effect on the validity and the outcome of the study. |
Description of key information
The test item dinotefuran technical had no adverse effects on the oxygen uptake at the highest concentration tested (1000 mg/L). Hence, the EC50 of dinotefuran technical is > 1000 mg/L and the NOEC is 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.