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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Oct. 8th 2003 to Oct. 17th 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
OECD Guidelines for the Testing of Chemicals No. 202, Part I; adopted April 4th 1984, Daphnia sp., Acute Immobilization Test and Reproduction Test" Part I -Acute Immobilization Test
Deviations:
yes
Remarks:
The test temperature in the tests laid above the limit of 22 °C (maximum 23.8 °C). As the results of the study showed no inconsistencies, this deviation was stated as uncritical.
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
EU-Guideline C.2. "Akute Toxizitat fur Daphnien", adopted July 31st 1992
Deviations:
yes
Remarks:
The test temperature in the tests laid above the limit of 22 °C (maximum 23.8 °C). As the results of the study showed no inconsistencies, this deviation was stated as uncritical.
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
No further details specified in the study report.
Analytical monitoring:
yes
Details on sampling:
Not specified
Vehicle:
no
Details on test solutions:
Pre-Test
Preparation of the test solutions
A stock solution containing 1000 mg/l in dilution water was prepared.
The stock solution then was used to prepare four concentrations in a geometric series, spaced by the factor 10.

Main Study
Preparation of the test solutions
The result of the pre-test led to the assumption that the EC50 should lie above 1000 mg/l. Therefore the main study was performed as a limit test. A solution containing 1000 mg/l was prepared.
Test organisms (species):
Daphnia magna
Details on test organisms:
Species: Daphnia magna
Variety: STRAUS
Strain: Berlin
Sex: female
Age: between 0 and 24 hours
Origin: Umweltbundesamt Berlin
Arrival of Strain: July 07th 2003

Breeding and Holding
Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical. The holding is performed similar to the method described in EN ISO 6341, following SOP 115 002 01 (,Zucht und Halterung von Daphnia magna STRAUS"), edition 5, adopted Jan. 13th 2003.
Vessels: glass beakers, nominal volume 21
Medium: M4-Medium (ELENDT)
Food: cold water fish food Tetra Aminin®, finely ground, suspended in M4-Medium and boiled for three seconds green algae (Desmodesmus subspicatus)
changing of medium: twice a week
photo period: 16/8 hours, using neon tubes
temperature: 20 ± 2 °C
Test type:
not specified
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
No post exposure observation period specified in the study report.
Hardness:
Not specified
Test temperature:
20 ± 2 °C (deviation within one test ± 1 °C)
pH:
7.6 - 8.3
Dissolved oxygen:
8.0 - 8.5 mgO2/L
Salinity:
Not specified
Conductivity:
Not specified
Nominal and measured concentrations:
Nominal concentrations
Details on test conditions:
Selection of Daphnia
24 hours before the start of the test, the adult animals were separated from the youngs.
23 hours later, the adults were caught with the help of a glass tube, and the newborn daphnia, aged between 0 and 23 hours, were sieved from the medium and immediately placed into a 250 ml-beaker containing dilution water. After a settling-in period of 30 minutes, animals which showed no apparent damage were used for the test.
There was no difference in the selection of animals in pre-test and main study.

Study Performance
Using a glass tube (diameter approx. 8 mm), the daphnia were caught and lifted from the beaker. They were put on a small sieve, and the medium surrounding the animals was sucked off using absorbent paper. Immediately after that, the animals were put into the respective test solution.
The test vessels were left to stand for 48 hours. After that period, the immobilized daphnia were counted. Daphnia are considered immobilized if they perform no movements or are only able to move their antennae when the beaker is gently agitated.
The pH and the concentration of dissolved oxygen in the test vessels were measured at the beginning and at the end of the test. In the main-study the real concentration of the test solutions were determined at the beginning and at the end of the test.
All values were documented in the raw data.

Pre-Test
Date: Oct. 08th - Oct. 10th 2003
Test concentrations: 1000 / 100 / 10 / 1 mg/l
Duration: 48 hours
Temperature: 20 ± 2 °C (deviation within the test ± 1 °C)
Vessels: glass beakers, nominal volume 50 ml, tall shape
Feeding: none
Lighting: none
Replicates: two vessels, each containing 20 ml test solution and 5 daphnia
Blank: two vessels, each containing 20 ml dilution water and 5 daphnia

Main Study
Date: Oct. 15th - Oct. 17th 2003
Test Concentrations: 1000 mg/l
Duration: 48 hours
Temperature: 20 ± 2 °C (deviation within one test ± 1 °C)
Vessels: glass beakers, nominal volume 50 ml, tall shape
Feeding: none
Lighting: none
Replicates: four vessels, each containing 20 ml test solution and 5 daphnia
Blank: four vessels, each containing 20 ml dilution water and 5 daphnia
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOELR
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
At the end of the test, the concentration of the test item in the treatment was determined as 97 % of the measured concentration at the beginning of the test. Therefore the test item can be stated as stable under test conditions. For this reason, the results are given in nominal concentrations.
Results with reference substance (positive control):
The 24h EC50 of K2Cr2O7 was determined as 1.2 mg/L.
Reported statistics and error estimates:
The data were evaluated using sigmoidal fit.

Pre-Test

Conc. in mg/L

Immobility abs.

Immobility in %

pH

t = 0 h

pH

t = 48 h

O2-conc. mg/L

t = 0 h

O2-conc. mg/L

t = 48 h

0

0

0

0

7.7

8.1

8.3

8.1

1000

0

0

0

7.8

8.2

8.3

8.0

100

0

0

0

7.8

8.1

8.3

8.1

10

0

0

0

7.8

8.1

8.2

8.1

1

0

0

0

7.6

7.9

8.3

8.0

 

Main Study

Conc. in mg/L

Immobility abs.

Immobility in %

pH

t = 0 h

pH

t = 48 h

O2-conc. mg/L

t = 0 h

O2-conc. mg/L

t = 48 h

0

0

0

0

0

0

7.9

8.2

8.5

8.4

1000

0

0

0

0

0

8.0

8.3

8.3

8.2

 

Analytical Determinations

Nom. Conc. in mg/L

Real Conc. in mg/L

% recovery after 48 h

0 h

48 h

0

0

0

--

1000

1018.78

984.87

96.67

The analytical determination of the test item in the test solution showed a very good recovery after 48 hours. Therefore the test item can be considered as stable under the test conditions.

 

Results

Parameter

Value

95%-confidence interval

NOEL 48 h

1000 mg/L

n.d.

48 h EC50

> 1000 mg/L

n.d.

48 h EC100

> 1000 mg/L

n.d.

 

 

Reference Test

Conc. in mg/L

Immobility abs.

Immobility in %

pH

t = 0 h

pH

t = 48 h

O2-conc. mg/L

t = 0 h

O2-conc. mg/L

t = 48 h

0

0

0

0

8.0

8.0

Not determined caused by a failure of the instrument

8.2

0.625

0

0

0

8.0

8.1

8.2

1.0

2

2

40

8.1

8.2

8.1

1.25

2

4

60

8.1

8.2

8.1

1.5

3

3

60

8.1

8.2

8.0

2.0

5

5

100

8.1

8.2

8.1

2.5

5

5

100

8.0

8.2

8.1

3.0

5

5

100

8.0

8.2

8.1

 

Results

Parameter

Value

95%-confidence interval

NOEL 24 h

0.625 mg/L

n.d.

24 h EC50

1.2 mg/L

n.d.

24 h EC100

2.0 mg/L

n.d.

 

Validity criteria fulfilled:
yes
Conclusions:
The following results were determined for the test item Glycerin-1-allylether in the acute toxicity test against Daphnia magna:
24h EC50 > 1000 mg/L
48h EC50 > 1000 mg/L
Executive summary:

In the initial experiment, four concentrations of the test item in a geometric series (spaced by factor 10, the highest being 1000 mg/L) were used. For each concentration, ten daphnia were exposed to the test item for 48 hours.

No concentration caused immobilization towards the daphnia.

None of the animals showed signs of morbidity.

 

The main study was performed as a limit test using the nominal concentration 1000 mg/L, which showed no effect on the daphnia. The real concentration of the test item in the treatment was determined before and after the test. The test item can be stated as stable under the test conditions.

Therefore the nominal concentration was used to estimate the results.

 

The following results were determined for the test item Glycerin-1-allylether in the acute toxicity test against Daphnia magna:

24h EC50 > 1000 mg/L

48h EC50 > 1000 mg/L

Description of key information

The following results were determined for the test item Glycerin-1-allylether in the acute toxicity test against Daphnia magna:

24h EC50 > 1000 mg/L

48h EC50 > 1000 mg/L

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
1 000 mg/L

Additional information

In the initial experiment, four concentrations of the test item in a geometric series (spaced by factor 10, the highest being 1000 mg/L) were used. For each concentration, ten daphnia were exposed to the test item for 48 hours.

No concentration caused immobilization towards the daphnia.

None of the animals showed signs of morbidity.

 

The main study was performed as a limit test using the nominal concentration 1000 mg/L, which showed no effect on the daphnia. The real concentration of the test item in the treatment was determined before and after the test. The test item can be stated as stable under the test conditions.

Therefore the nominal concentration was used to estimate the results.

 

The following results were determined for the test item Glycerin-1-allylether in the acute toxicity test against Daphnia magna:

24h EC50 > 1000 mg/L

48h EC50 > 1000 mg/L