4-(2-aminoethyl)phenol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

Data is from publication.

Data source

Reference

Reference Type:

publication

Title:

Inhibitory Effects of Tea Extracts on the Mutagenicity of 1-Methyl-1,2,3,4-tetrahydro- b-carboline-3-carboxylic Acid on Treatment with Nitrite in the Presence of Ethanol

Author:

M. HIGASHIMOTO1, Y. AKADA1, M. SATO1, T. KINOUCHI2, T. KUWAHARA2 and Y. OHNISHI2

Year:

2000

Bibliographic source:

Food and Chemical Toxicology 38 (2000) 7-13

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: As mention below

Principles of method if other than guideline:

To evaluate the mutagenic potential of Tyramine in Salmonella typhimurium Strain TA100 by AMES assay.

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

4-(2-aminoethyl)phenol

EC Number:

200-115-8

EC Name:

4-(2-aminoethyl)phenol

Cas Number:

51-67-2

Molecular formula:

C8H11NO

IUPAC Name:

4-(2-aminoethyl)phenol

Test material form:

solid: crystalline

Details on test material:

- Name of test material : 4-(2-aminoethyl) phenol
- Common name : Tyramine
- Molecular formula : C8H11NO
- Molecular weight : 137.1809 g/mol
- Smiles notation : NCCc1ccc(O)cc1
- InChl : 1S/C8H11NO/c9-6-5-7-1-3-8(10)4-2-7/h1-4,10H,5-6,9H2
- Substance type: Organic
- Physical state: Solid

Specific details on test material used for the study:

- Name of test material : 4-(2-aminoethyl) phenol
- Common name : Tyramine
- Molecular formula : C8H11NO
- Molecular weight : 137.1809 g/mol
- Smiles notation : NCCc1ccc(O)cc1
- InChl : 1S/C8H11NO/c9-6-5-7-1-3-8(10)4-2-7/h1-4,10H,5-6,9H2
- Substance type: Organic
- Physical state: Solid

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

S. typhimurium TA 100

Details on mammalian cell type (if applicable):

Not applicable.

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

not specified

Metabolic activation:

without

Metabolic activation system:

S9 mix

Test concentrations with justification for top dose:

20 µg/plate

Vehicle / solvent:

Not specified

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: 2-(2-furyl)-3-(5-nitro-2-furyl)-acryl- amide (AF-2, 10 ng)

Details on test system and experimental conditions:

Details on test system and conditions
METHOD OF APPLICATION: Preincubation Method
DURATION
- Preincubation period: 20 minutes
- Exposure duration: 48 hour

NUMBER OF REPLICATIONS: Duplicate

Rationale for test conditions:

Not specified

Evaluation criteria:

The number of His+ revertants colonies was counted .

Statistics:

The data were analyzed statistically by analysis of variance. The correlation coefficient was calculated by the formula of Pearson.

Results and discussion

Test results

Species / strain:

S. typhimurium TA 100

Metabolic activation:

without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

The mutation was assayed in brown tubes under the yellow lamp in the dark room. The numbers of spontaneous revertants (112±16) were subtracted from all the data on mutagenicity.
A positive control 2-(2-furyl)-3-(5-nitro-2-furyl)-acryl- amide (AF-2, 10 ng) generated 427±65 His+ revertants.

Remarks on result:

other: Mutagenic effect were observed .

Applicant's summary and conclusion

Conclusions:

Tyramine (51-67-2) was evaluated for its mutagenic potential in Salmonella typhimurium Strain TA100 by AMES assay. The test result was considered to be positive in the absence of S9 mix.

Executive summary:

Genetic toxicity study in vitro was assessed for its possible mutagenic potential. For this purpose AMES assay was performed in Salmonella typhimurium Strain TA100 by usingPreincubation Method. The test substance was exposed at the concentration of20 µg/plate in the absence of S9 mix. Mutagenic effects were observed. The AMES test must be performed with and without S9 mix. For a substance to be a mutagen, AMES test should be positive with and without S9 mix. But as in this study the test was performed only in the absence of S9. The test result was considered to be inconclusive as no data available in the presence of S9. Hence further testing should be performed as data is insufficient to classify the substance.