Registration Dossier

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1967-07-04 - 1967-07-11
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: basic information given

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1967
Report Date:
1967

Materials and methods

Principles of method if other than guideline:
according to BASF-internal standard
GLP compliance:
no
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
mouse
Strain:
other: "Kisslegg" mice
Sex:
male/female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Details on exposure:
Form of application: solution
Concentrations used: 2, 4, 8 and 16% (w/v)
Doses:
200, 250, 320, 400, 500, 800 and 1600 mg/kg
No. of animals per sex per dose:
10 per dose level
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 400 mg/kg bw
Mortality:
200, 250 and 320 mg/kg: no deaths; 400 mg/kg: 5/10 after 7 days; 500 mg/kg: 8/10 after 7 days; 800 and 1600 mg/kg: 10/10 after 7 days
Clinical signs:
Dyspnea, apathy, staggering, abdominal and lateral position, convulsions
Gross pathology:
Animals that died: 1 x adherences between liver and stomach
Sacrificed animals: mot animals with adherences or adhesions, 3 x spotty-paled liver

Applicant's summary and conclusion