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EC number: 268-597-2 | CAS number: 68130-55-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 Aug 2017 - 07 Nov 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 490 (In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- in vitro mammalian cell gene mutation tests using the thymidine kinase gene
Test material
- Reference substance name:
- Hexanedioic acid, mixed esters with decanoic acid, heptanoic acid, octanoic acid and pentaerythritol
- EC Number:
- 268-597-2
- EC Name:
- Hexanedioic acid, mixed esters with decanoic acid, heptanoic acid, octanoic acid and pentaerythritol
- Cas Number:
- 68130-55-2
- Molecular formula:
- C36H64O10
- IUPAC Name:
- 6-[3-(decanoyloxy)-2-[(heptanoyloxy)methyl]-2-[(octanoyloxy)methyl]propoxy]-6-oxohexanoic acid
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Based on the information provided by sponsor, the test item is considered as an Unknown
or Variable Composition, Complex Reaction products and Biological materials (UVCBs),
test item is able to be well dissolved in acetone.
EC#268-597-2, CAS #68130-55-2
Purity: >99% I UVCB, Homogenous liquid
Pale yellow liquid, no odour
Method
- Target gene:
- thymidine kinase gene
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Details on mammalian cell type (if applicable):
- The test system used in the study was L5178Y TK+1
- clone (3.7.2C) from Mus
musculus (mouse) lymphoma (ATCC®CRL-9518™), passage 7.
- Metabolic activation:
- with and without
- Metabolic activation system:
- The cofactor supplemented post-mitochondrial fraction (S9) prepared from the liver of Aroclor 1254 induced Sprague-Dawley rats was used as metabolic activation system.
- Test concentrations with justification for top dose:
- To prepare test substances, the \est item was dissolved in acetone at concentration of 60%,
20%, 6. 7% and 2.3% (v/v) respectively, four concentrations in total. The concentration
interval is approximately 3. - Vehicle / solvent:
- Acetone
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Details on test system and experimental conditions:
- The test system used in the study was L5178Y TK+/- clone (3.7.2C) from Mus
musculus (mouse) lymphoma (ATCC®CRL-9518™), passage 7.
L5178Y TK+1- cells were maintained in the complete growth medium R1 OP [ROP
(RPMI 1640 Medium containing 2g/L Sodium Bicarbonate, 0.1% Poloxamer 188, 1 mM Sodium Pyruvate and 1 % Penicillin-Streptomycin) with 10% Fetal Bovine Serum (FBS)] in exponentially proliferating status at 37 °C, 5% C02.
L5178Y TK+/- cells were sub-cultured in the complete growth medium R10P
1- day before being used for treatment. 6 x 10E6 cells were used for each
treatment. The complete growth medium R1 OP was replaced by treatment
medium R3P (ROP with 3% FBS) with test substances, negative control
substances, and positive control substances so as to expose the cells to these
substances.
The exposure was performed with and without metabolic activation. The cofactor
supplemented post-mitochondrial fraction (S9) prepared from the liver
of Aroclor 1254 induced Sprague-Dawley rats was used as metabolic
activation system. The concentration of S9 fraction in the S9 mixture was 10%
(v/v) while the concentration of S9 fraction in final test medium was 1 %.
The exposure was conducted for 3 hrs. After the exposure, the above
solutions were removed, the cells were washed, added with fresh complete
growth medium R10P at seeding density of 30 x 10E4 cells/ml and then
incubated for another 48 hrs at 37 °C in 5% C02 incubator. During 2-day
expression, the cells was counted on each day and cell density was adjusted
to 30 x 10E4 cells/ml after Day 1 counting.
Results and discussion
Test results
- Key result
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Under the condition of this study, the above results show that, using acetone as solvent, the test item -
Mixed esters with CAS No. 68130-55-2, Batch or Lot No: 026X6 is non-mutagenic to the L5178Y TK +/-
clone (3.7.2C) cells at 3-hour treatment both in the absence and presence of S9 metabolic activation
system. - Executive summary:
At 3-hour treatment with and withous S9, MF of negative control substance is within the range of
35 to 140 x 10E-6 , cloning efficiency of negative control substance is within the range of 65-120%,
suspension growth of negative control substance is within the range of 8-32 fold, which indicates
acceptability of data.
Induced MF (IMF) of positive control is more than 300 x 1 over the concurrent negative control,
IMF of small colony of positive control is 265 x 10E-6 (> 150 x 10E-6), which indicates positive
response.
At 3-hour treatment with and without S9, there is no mutant frequency (MF) of test substances
more than 90 x 10 E-6 (Global Evaluation Factor) over the concurrent negative control, which
indicates no positive response in all test substances.
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