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EC number: 204-429-6 | CAS number: 120-83-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: LIterature report of a proprietary study. Awarded a reliability score of 2 in the source report.
Data source
Reference
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- not specified
- Principles of method if other than guideline:
- according to Boller et Schmid (1970 HumanGenetik 11, 35-54) and Schmid (1975 Mutation Res., 9-15)
- GLP compliance:
- no
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 2,4-dichlorophenol
- EC Number:
- 204-429-6
- EC Name:
- 2,4-dichlorophenol
- Cas Number:
- 120-83-2
- Molecular formula:
- C6H4Cl2O
- IUPAC Name:
- 2,4-dichlorophenol
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- Swiss
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- no data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- Peanut oil
- Details on exposure:
- Immediately before dosing
- Duration of treatment / exposure:
- 48 hour from first dose to sacrifice
- Frequency of treatment:
- Mice were dosed twice with a 24 hour interval between.
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
0 mg/kg (vehicle only)
Basis:
actual ingested
gavage
- Remarks:
- Doses / Concentrations:
160 mg/kg
Basis:
actual ingested
gavage
- Remarks:
- Doses / Concentrations:
800 mg/kg
Basis:
actual ingested
gavage
- No. of animals per sex per dose:
- 10
- Positive control(s):
- methylmethanesulfonate at 65 mg/kg, or cis-platin at 10 mg/kg by gavage
Examinations
- Statistics:
- students t-test
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- 800 mg/kg/d produced severe prostration.
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
2,4-dichlorophenol was negative in this in vivo mouse micronucleus assay. - Executive summary:
In a bone marrow micronucleus assay, Swiss mice (10/dose) were treated by gavage with 2,4-dichlorophenol at doses of 0, 160 and 800 mg/kg bw/day for 2 days (24 hours between doses). Bone marrow cells were harvested 24 hours after the second dose. The vehicle was peanut oil. The positive controls were methylmethanesulfonate and cisplatin.
The only sign of toxicity was severe prostration at 800 mg/kg during the study. 2,4-dichlorophenol was tested at an adequate dose based on the signs of toxicity seen. The positive control induced the appropriate response. There was no significant increase in the frequency of micronucleated polychromatic erythrocytes in bone marrow after any treatment time.
This study satisfies, as far as can be discerned the requirements for Test Guideline OECD 474 for in vivo cytogenetic mutagenicity data.
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