p,p',p''-tris(dimethylamino)trityl alcohol

Endpoint summary

Description of key information

Biodegradation in water:

Biodegradation study was conducted for 28-days Closed Bottle test following the OECD guideline 301 D for determining the ready biodegradability of the test chemical. Polyseed capsule ( blend of broad spectrum bacteria) designed specifically as seed inoculums for the BOD test. 38.4 mg of polyseed from the capsule was weighed and added in 100 mL mineral media and then stirred for 1 h. This gave the bacterial count as 10E4 to 10E6 CFU/L. A further concentration of 1 mL/L of polyseed solution is prepared and from this working inoculum 0.125 mL solution is added to every bottles of 125 mL. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism.125 mL BOD vessels were used in the study. The test system includes an inoculum blank control group, solvent control, a procedure control group, and a test item group, a toxicity control group, each maintained in replicates. The biodegradation was determined by following the BOD, oxygen consumption of the test item in the incubation BOD vessels during exposure. Sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. All test vessels were incubated at BOD incubator at a constant temperature of 20±1° C. Sampling of 0^th h test vessels from each test group concentration were collected for analysis at zero-time (immediately after set-up). Samples were removed at regular intervals i.e., 7th, 14th, 21st, 28th, 39th and 42nd day during the incubation period which were measured for D.O and pH also. The theoretical oxygen demand (ThOD) was calculated based on the chemical identifier. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 73.65%. Degradation of Sodium Benzoate exceeds 52.69% on 7 days & 61.67% on 14th day, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the procedure control sodium benzoate, 37.8% biodegradation was noted after 42 days of incubation. Thus, the test item can be assumed to be not inhibitory to the activated sludge microorganisms. Under the test conditions the percentage biodegradation of test chemical reached 30.48% after 42 days of incubation based on BOD consumption. As the final biodegradation is less than 70% in this test and all validity criteria were met, test item was considered to be inherently biodegradablein water.

Biodegradation in water and sediment:

Estimation Programs Interface prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 3.58% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemicxal in water was estimated to be 180 days (4320 hrs). The half-life (180 days estimated by EPI suite) indicates that the chemical is persistent in water and the exposure risk to aquatic animals is moderate to high whereas the half-life period of test chemical in sediment was estimated to be 1620.833 days (38900 hrs). Based on this half-life value, it indicates that test chemical is persistent in sediment.

Biodegradation in soil:

The half-life period of test chemical in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database. If released into the environment, 90.9% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemicalin soil is estimated to be 360 days (8640 hrs). Based on this half-life value of test chemical, it is concluded that the chemical is persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to high.

Additional information

Biodegradation in water:

Biodegradation study was conducted for 28-days Closed Bottle test following the OECD guideline 301 D for determining the ready biodegradability of the test chemical. Polyseed capsule ( blend of broad spectrum bacteria) designed specifically as seed inoculums for the BOD test. 38.4 mg of polyseed from the capsule was weighed and added in 100 mL mineral media and then stirred for 1 h. This gave the bacterial count as 10E4 to 10E6 CFU/L. A further concentration of 1 mL/L of polyseed solution is prepared and from this working inoculum 0.125 mL solution is added to every bottles of 125 mL. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism.125 mL BOD vessels were used in the study. The test system includes an inoculum blank control group, solvent control, a procedure control group, and a test item group, a toxicity control group, each maintained in replicates. The biodegradation was determined by following the BOD, oxygen consumption of the test item in the incubation BOD vessels during exposure. Sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. All test vessels were incubated at BOD incubator at a constant temperature of 20±1° C. Sampling of 0^th h test vessels from each test group concentration were collected for analysis at zero-time (immediately after set-up). Samples were removed at regular intervals i.e., 7th, 14th, 21st, 28th, 39th and 42nd day during the incubation period which were measured for D.O and pH also. The theoretical oxygen demand (ThOD) was calculated based on the chemical identifier. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 73.65%. Degradation of Sodium Benzoate exceeds 52.69% on 7 days & 61.67% on 14th day, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the procedure control sodium benzoate, 37.8% biodegradation was noted after 42 days of incubation. Thus, the test item can be assumed to be not inhibitory to the activated sludge microorganisms. Under the test conditions the percentage biodegradation of test chemical reached 30.48% after 42 days of incubation based on BOD consumption. As the final biodegradation is less than 70% in this test and all validity criteria were met, test item was considered to be inherently biodegradablein water.

Biodegradation in water and sediment:

Estimation Programs Interface prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 3.58% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemicxal in water was estimated to be 180 days (4320 hrs). The half-life (180 days estimated by EPI suite) indicates that the chemical is persistent in water and the exposure risk to aquatic animals is moderate to high whereas the half-life period of test chemical in sediment was estimated to be 1620.833 days (38900 hrs). Based on this half-life value, it indicates that test chemical is persistent in sediment.

Biodegradation in soil:

The half-life period of test chemical in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database. If released into the environment, 90.9% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemicalin soil is estimated to be 360 days (8640 hrs). Based on this half-life value of test chemical, it is concluded that the chemical is persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to high.