Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-942-2 | CAS number: 112-17-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Decyl acetate
- EC Number:
- 203-942-2
- EC Name:
- Decyl acetate
- Cas Number:
- 112-17-4
- Molecular formula:
- C12H24O2
- IUPAC Name:
- decyl acetate
- Test material form:
- liquid
Constituent 1
Method
- Target gene:
- Histidine
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535
- Details on mammalian cell type (if applicable):
- - Histidine mutation: hisG46
- Additional Mutation Repair LPS-Coat: uvrB- rfa
- Mutation type detected: Base Pair
- Species / strain / cell type:
- S. typhimurium TA 98
- Details on mammalian cell type (if applicable):
- - Histidine mutation: hisD3052
- Additional Mutation Repair LPS-Coat: uvrB- rfa
- Affecting R-Factor: pKM101
- Mutation type detected: Frameshift
- Species / strain / cell type:
- S. typhimurium TA 1537
- Details on mammalian cell type (if applicable):
- - Histidine mutation: hisC3076
- Additional Mutation Repair LPS-Coat: uvrB- rfa
- Mutation type detected: Frameshift
- Species / strain / cell type:
- S. typhimurium TA 102
- Details on mammalian cell type (if applicable):
- - Histidine mutation: hisAG8476 rfa, hisG428 on plasmid pAQ1
- Affecting R-Factor: pKM101, + pAQ1
- Mutation type detected: Base Pair Substitution
- Species / strain / cell type:
- S. typhimurium TA 100
- Details on mammalian cell type (if applicable):
- - Histidine mutation: hisG46
- Additional Mutation Repair LPS-Coat: uvrB-
- Affecting R-Factor: pKM101
- Mutation type detected: Substitution
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver homogenates (S9)
- Test concentrations with justification for top dose:
- 1.5 - 1500 µg/plate in presence of S9 and 1.5 - 5000 µg/plate in the absence of S9
- Vehicle / solvent:
- - Vehicle: ethanol
Controls
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- mitomycin C
- other: 2-aminoantracene
- Remarks:
- 9-Aminoacridine, mitomycin C, and sodium azide were dissolved in distilled water. 2-Aminoanthracene, and 2-nitrofluorene were dissolved in DMSO.
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation): Vogel-Bonner minimal plates enriched with histidine (264M), biotin (3p.M) and ampicillin.
DURATION
- Exposure duration: Plates were kept for 48 to 72 h at 37°C in the dark
EXAMINATION:
- counting of the number of revertant colonies (his+revertants)
- normal background lawn and/or precipitates
- microscopically: microcolony growth
- genotype of revertant colonies are spot-checked by picking and streaking on histidine free plates, if relevant.
OTHER: The experiment was repeated in full after an interval of at least 3 days.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- without metabolic activation at 500 µg/plate, with at 1500 µg/plate
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- with and without metabolic activation at 500 µg/plate
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- without metabolic activation at 1500 µg/plate, with at 500 µg/plate
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- without metabolic activation at 5000 µg/plate, with at 1500 µg/plate
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 1500 µg/plate
- Positive controls validity:
- valid
- Additional information on results:
- The results with the positive control substances confirmed the known reversion properties and specificity of the tester strains as well as the full activity of the metabolizing system.
Applicant's summary and conclusion
- Conclusions:
- The results indicate that test item was not mutagenic to Salmonella typhimurium strains TA1535, TA1537, TA98, TA100, and TA102 in the presence and absence of a metabolizing system, under the experimental conditions described. No precipitation was observed.
- Executive summary:
In the present study, the substance was investigated using the standard plate incorporation procedure according to Ames et al., (1975) (OECD 471). The number of revertant colonies on the plates, with and without the test compound, were compared to evaluate mutagenicity.
The mutagenicity of the test substance was studied with five mutant strains of Salmonella typhimurium (TA1535, TA1537, TA98, TA100, and TA102). The investigations were carried out using the standard plate incorporation assay with and without liver homogenate (S9) from Aroclor 1254 pretreated male rats as metabolic activation system.
The test item was dissolved in ethanol and tested in concentrations of 1.5 to 1500 µg per plate in the presence of S9 and 1.5 to 5000 µg per plate in the absence of S9.
In the absence of S9-mix the test item was bacteriotoxic towards the strains TA100 (500 µg/plate), TA102 (500 µg/plate), TA1537 (1500 µg/plate) and TA98 (5000 µg/plate).
In the presence of S9-mix the substance was bacteriotoxic towards the strains TA1537 (500 µg/plate), TA102 (500 µg/plate), TA1535 (1500 µg/plate), TA98 (1500 µg/plate), and TA100 (1500 µg/plate).
Precipitation of the test compound on the plates was not observed.
Sodium azide, 2-nitrofluorene, 9-aminoacridine, mitomycin C, and 2-aminoanthracene served as positive controls to confirm the reversion properties and the specificity of the bacterial strains as well as the efficacy of the metabolizing system.
In the concentration range investigated, the test substance did not induce a significant increase in the mutation frequency of the test strains in the presence and absence of a metabolic activation system.
In conclusion, these results indicate that the test substance, under the experimental conditions described, was not mutagenic to Salmonella typhimurium strains TA1535, TA1537, TA98, TA100, and TA102 in the presence and absence of a metabolizing system.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.