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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
Safety assessment of α-amino acids as used in cosmetics.
Author:
Cosmetic Ingredient Review Expert Panel
Year:
2013
Bibliographic source:
International Journal of Toxicology; 2013 Nov-Dec;32(6 Suppl):41S-64S.

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
A Draize test was conducted in 4 male New Zealand White albino rabbits to assess the eye irritation potential of chemical Arginine (CAS no: 74-79-3).
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Arginine
EC Number:
200-811-1
EC Name:
Arginine
Cas Number:
74-79-3
Molecular formula:
C6H14N4O2
IUPAC Name:
Arginine
Test material form:
solid
Details on test material:
- Name of test material (as cited in study report): Arginine
- Molecular formula: C6H14N4O2
- Molecular weight: 174.2 g/mol
- Substance type: Organic
- Physical state: Solid
- Smiles: C(CCNC(N)=N)[C@@H](C(O)=O)N
-InChI: 1S/C6H14N4O2/c7-4(5(11)12)2-1-3-10-6(8)9/h4H,1-3,7H2,(H,11,12)(H4,8,9,10)/t4-/m0/s1
Specific details on test material used for the study:
- Name of test material (as cited in study report): Arginine
- Molecular formula: C6H14N4O2
- Molecular weight: 174.2 g/mol
- Substance type: Organic
- Physical state: Solid
- Smiles: C(CCNC(N)=N)[C@@H](C(O)=O)N
-InChI: 1S/C6H14N4O2/c7-4(5(11)12)2-1-3-10-6(8)9/h4H,1-3,7H2,(H,11,12)(H4,8,9,10)/t4-/m0/s1

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
No data available

Test system

Vehicle:
other: Distilled water
Controls:
not specified
Amount / concentration applied:
5%
Duration of treatment / exposure:
No data available
Observation period (in vivo):
No data available
Number of animals or in vitro replicates:
4 (male )
Details on study design:
No data available

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
No eye irritation was observed.

Applicant's summary and conclusion

Interpretation of results:
other: Not irritating
Conclusions:
The test material Arginine (CAS no: 74-79-3) was considered to be not irritating to the eyes of New Zealand White rabbits.
Executive summary:

A Draize test was conducted in 4 male New Zealand White albino rabbits to assess the eye irritation potential of chemical Arginine (CAS no: 74-79-3).

 

About 5% of Arginine was installed into the eye of each rabbit and later observed for any signs of ocular lesions.

 

None of the treated rabbits showed any known signs of eye irritation.

Hence the chemical Arginine (CAS no: 74-79-3)was considered to be not irritating to the eyes of New Zealand White rabbits.