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EC number: 201-714-7 | CAS number: 86-98-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed journals
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity testing of the test chemical and its various derivatives
- Author:
- Minako Nagao et.al
- Year:
- 1 977
- Bibliographic source:
- Mutation Research, 1977
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Bacterial reverse mutation assay was performed to evaluate the toxic nature of the test chemical on Salmonella typhimurium TA98 and TA100 strains.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4,7-dichloroquinoline
- EC Number:
- 201-714-7
- EC Name:
- 4,7-dichloroquinoline
- Cas Number:
- 86-98-6
- Molecular formula:
- C9H5Cl2N
- IUPAC Name:
- 4,7-dichloroquinoline
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Name of test material: 4,7 Dichloroquinoline
- IUPAC name: 4, 7 Dichloroquinoline
- Molecular formula: C9H5Cl2N
- Molecular weight: 198.051 g/mole
- Smiles : c12c(cc(Cl)cc2)nccc1Cl
- Inchl: 1S/C9H5Cl2N/c10-6-1-2-7-8(11)3-4-12-9(7)5-6/h1-5H
- Substance type: Organic
- Physical state: Solid powder (pale cream)
- Purity: 99.4%(by GC)
Constituent 1
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA100 and TA 98
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- other: Base-pair-change mutant and frameshift mutant. Both strains have the R-factor of pKM101 as a plasmid
- Cytokinesis block (if used):
- No data
- Metabolic activation:
- with and without
- Metabolic activation system:
- Type and composition of metabolic activation system:
The S-9 fraction was prepared aseptically by centrifugation of the liver homogenate (25% in 0.15 M KCl) at 9000 g for 10 min as described by Ames et al.
- source of S9 : Male Sprague-Dawley rats weighing 100-120 g were injected intraperitoneally with polychlorinated biphenyl (Kanechlor 500) at a dose of 50 mg/100 g body weight, five days before they were killed
- concentration or volume of S9 mix and S9 in the final culture medium : S-9 mix contained 50 µmol of sodium phosphate buffer (pH 7.4), 4 µmol of MgCl2, 16.5 µmol of KCI, 2.5µmol of glucose-6-phosphate, 2 µmol of NADH, 2 µmol of NADPH, 2.5 µmol of ATP and 150µmol of S-9 fraction in a total volume of 0.5 ml. - Test concentrations with justification for top dose:
- 0, 0.5 or 1.0 µmoles/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: The test chemical was stable and soluble in DMSO.
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- NUMBER OF REPLICATIONS:
- Number of cultures per concentration (single, duplicate, triplicate) : no data available
- Number of independent experiments : no data available
METHOD OF TREATMENT/ EXPOSURE:
- Cell density at seeding (if applicable):
- Test substance added in medium; in agar (plate incorporation); preincubation; in suspension; as impregnation on paper disk : pre-incubation
TREATMENT AND HARVEST SCHEDULE
- Preincubation period, if applicable: 20 minutes
- Exposure duration/duration of treatment: 2 days
- Harvest time after the end of treatment (sampling/recovery times):
- Rationale for test conditions:
- No data
- Evaluation criteria:
- After 2-day incubation, colonies of histidine prototroph were counted as revertants.
- Statistics:
- No data
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: Salmonella typhimurium TA100 and TA 98
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- True negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- other: S. typhimurium TA 100, S. typhimurium TA 98
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- True negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- Ames test: The test chemical was weakly mutagenic to both strains with S-9 mix and non mutagenic to both strains in the absence of S9 mix. Though there was weak mutagenic activity in the presence of S9 mix but dose dependent decrease in number of revertants was observed. Hence, we consider the test chemical can be considered to be non-mutagenic both in the presence and absence of S9 mix to S.Typhimurium TA 98 and TA 100 strains.
- Remarks on result:
- other: non-mutagenic
Applicant's summary and conclusion
- Conclusions:
- The test chemical was weakly mutagenic to both strains with S-9 mix and non mutagenic to both strains in the absence of S9 mix. Though there was weak mutagenic activity in the presence of S9 mix but dose dependent decrease in number of revertants was observed. Hence, the test chemical can be considered to be non-mutagenic both in the presence and absence of S9 mix to S.Typhimurium TA 98 and TA 100 strains.
- Executive summary:
In vitro genetic toxicity study was performed to determine the mutagenic nature of the test chemical. Strains TA100 and TA98 of Salmonella typhimirium were used as the tester strains. Assay was carried out as described by Ames et al. with some modifications. The test chemical was freshly dissolved in 100 µl of DMSO were pre-incubated at 37°C for 20 min with 0.5 ml of S-9 mix or 0.5 ml of 0.1 M sodium phosphate buffer (pH 7.4) and 0.1 ml of bacterial culture. Two ml of molten soft agar at 45°C were added, and the resulting mixture was poured over 25 ml of minimal-glucose agar containing 0.1 µmol of L-histidine and 0.1 µmol of biotin. After 2-day incubation, colonies of histidine prototroph were counted as revertants. The test chemical was weakly mutagenic to both strains with S-9 mix and non mutagenic to both strains in the absence of S9 mix. Though there was weak mutagenic activity in the presence of S9 mix but dose dependent decrease in number of revertants was observed. Hence, the test chemical can be considered to be non-mutagenic both in the presence and absence of S9 mix to S.Typhimurium TA 98 and TA 100 strains.
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