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EC number: 695-748-3
CAS number: 65286-55-7
Cumulative Immobilization Data and Observations in the Definitive Test
Cumulative Immobilized Daphnia(Initial Population: 5 Per Replicate)
R1 – R4 = Replicates 1 to 4
N = Normal
A study was performed to assess the acute toxicity of the test
item to Daphnia magna. The method followed was designed to be
compatible with the OECD Guidelines for Testing of Chemicals (April
2004) No 202, "Daphnia sp., Acute Immobilisation Test"
referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.
Following a preliminary range‑finding test, 20 daphnids
(4 replicates of 5 animals) were exposed to an aqueous solution of the
test item at nominal concentrations of 10, 18, 32, 56 and 100 mg/L for
48 hours at a temperature of approximately 21 °C to 22 °C under static
test conditions. The number of immobilized daphnia were recorded after
24 and 48 hours.
Analysis of the fresh test preparations at 0 hours showed measured
test concentrations to range from 9.5 to 104 mg/L. Analysis of the old
test media at 48 hours showed measured test concentrations to range from
11 to 103 mg/L. As all measured concentrations were within 20% of the
nominal concentrations it was considered justifiable to base the results
on the nominal test concentrations. Based on nominal
concentrations, the 48-h EC50 was determined to be 46 mg/L with a 95%
confidence interval ranging from 40 to 54 mg/L.
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